| General information | | DisProt: | DP00102 | | Name: | Myosin-11 | | Synonym(s): | MYH11_CHICK
Myosin heavy chain 11
Myosin heavy chain, gizzard smooth muscle
| | First appeared in release: | Release 2.0 (02/14/2005) | | UniProt: | P10587 | | UniGene: | Gga.3225 | | SwissProt: | MYH11_CHICK | | TrEMBL: | | | NCBI (GI): | 3915778 | | Source organism: | Gallus gallus (Chicken) | | Sequence length: | 1979 | | Percent disordered: | 6% | | Homologues: | |
| Native sequence |
10 20 30 40 50 60
| | | | | |
MSQKPLSDDE KFLFVDKNFV NNPLAQADWS AKKLVWVPSE KHGFEAASIK EEKGDEVTVE - 60
LQENGKKVTL SKDDIQKMNP PKFSKVEDMA ELTCLNEASV LHNLRERYFS GLIYTYSGLF - 120
CVVINPYKQL PIYSEKIIDM YKGKKRHEMP PHIYAIADTA YRSMLQDRED QSILCTGESG - 180
AGKTENTKKV IQYLAVVASS HKGKKDTSIT QGPSFSYGEL EKQLLQANPI LEAFGNAKTV - 240
KNDNSSRFGK FIRINFDVTG YIVGANIETY LLEKSRAIRQ AKDERTFHIF YYLIAGASEQ - 300
MRNDLLLEGF NNYTFLSNGH VPIPAQQDDE MFQETLEAMT IMGFTEEEQT SILRVVSSVL - 360
QLGNIVFKKE RNTDQASMPD NTAAQKVCHL MGINVTDFTR SILTPRIKVG RDVVQKAQTK - 420
EQADFAIEAL AKAKFERLFR WILTRVNKAL DKTKRQGASF LGILDIAGFE IFEINSFEQL - 480
CINYTNEKLQ QLFNHTMFIL EQEEYQREGI EWNFIDFGLD LQPCIELIER PTNPPGVLAL - 540
LDEECWFPKA TDTSFVEKLI QEQQNHAKFQ KSKQLKDKTE FCILHYAGKV TYNASAWLTK - 600
NMDPLNDNVT SLLNQSSDKF VADLWKDVDR IVGLDQMAKM TESSLPSASK TKKGMFRTVG - 660
QLYKEQLTKL MTTLRNTNPN FVRCIIPNHE KRAGKLDAHL VLEQLRCNGV LEGIRICRQG - 720
FPNRIVFQEF RQRYEILAAN AIPKGFMDGK QACILMIKAL ELDPNLYRIG QSKIFFRTGV - 780
LAHLEEERDL KITDVIIAFQ AQCRGYLARK AFAKRQQQLT AMLVIQRNCA AYLKLRNWQW - 840
WRLFTKVKPL LQVTRQEEEM QAKDEELQRT KERQQKAEAE LKELEQKHTQ LCEEKNLLQE - 900
KLQAETELYA EAEEMRVRLA AKKQELEEIL HEMEARIEEE EERSQQLQAE KKKMQQQMLD - 960
LEEQLEEEEA ARQKLQLEKV TADGKIKKME DDILIMEDQN NKLTKERKLL EERVSDLTTN - 1020
LAEEEEKAKN LTKLKNKHES MISELEVRLK KEEKSRQELE KIKRKLEGES SDLHEQIAEL - 1080
QAQIAELKAQ LAKKEEELQA ALARLEDETS QKNNALKKIR ELESHISDLQ EDLESEKAAR - 1140
NKAEKQKRDL SEELEALKTE LEDTLDTTAT QQELRAKREQ EVTVLKRALE EETRTHGAQV - 1200
QEMRQKHTQA VEELTEQLEQ FKRAKANLDK TKQTLEKDNA DLANEIRSLS QAKQDVEHKK - 1260
KKLEVQLQDL QSKYSDGERV RTELNEKVHK LQIEVENVTS LLNEAESKNI KLTKDVATLG - 1320
SQLQDTQELL QEETRQKLNV TTKLRQLEDD KNSLQEQLDE EVEAKQNLER HISTLTIQLS - 1380
DSKKKLQEFT ATVETMEEGK KKLQREIESL TQQFEEKAAS YDKLEKTKNR LQQELDDLVV - 1440
DLDNQRQLVS NLEKKQKKFD QMLAEEKNIS SKYADERDRA EAEAREKETK ALSLARALEE - 1500
ALEAKEELER TNKMLKAEME DLVSSKDDVG KNVHELEKSK RTLEQQVEEM KTQLEELEDE - 1560
LQAAEDAKLR LEVNMQAMKS QFERDLQARD EQNEEKRRQL LKQLHEHETE LEDERKQRAL - 1620
AAAAKKKLEV DVKDLESQVD SANKAREEAI KQLRKLQAQM KDYQRDLDDA RAAREEIFAT - 1680
ARENEKKAKN LEAELIQLQE DLAAAERARK QADLEKEEMA EELASANSGR TSLQDEKRRL - 1740
EARIAQLEEE LDEEHSNIET MSDRMRKAVQ QAEQLNNELA TERATAQKNE NARQQLERQN - 1800
KELRSKLQEM EGAVKSKFKS TIAALEAKIA SLEEQLEQEA REKQAAAKTL RQKDKKLKDA - 1860
LLQVEDERKQ AEQYKDQAEK GNLRLKQLKR QLEEAEEESQ RINANRRKLQ RELDEATESN - 1920
DALGREVAAL KSKLRRGNEP VSFAPPRRSG GRRVIENATD GGEEEIDGRD GDFNGKASE
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| Functional narrative |
The protein myosin is important in muscle contraction. It is located in the myofibril filaments of muscle tissue. Myosin consists of a lever arm and a head portion that binds weakly to actin, which is in a relatively fixed position. The lever arm of the myosin undergoes a conformational change which makes the head portion move in a swinging manner, known as a power stroke. These strokes cause very small movements approximately 10nm in length, which in turn cause the muscle to contract. The speed and strength of these contractions are believed to be dependent upon a 7 amino acid insert in the 25/50 kDa junction of myosin. Myosin containing the insert is capable of faster and stronger contractions than isoforms without the insert.
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| Map of ordered and disordered regions |
Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.
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| Region 1 | | Type: | Disordered | | Name: | | | Location: | 1 - 33 | | Length: | 33 | | Region sequence: |
MSQKPLSDDEKFLFVDKNFVNNPLAQADWSAKK | | Modification type: | Engineered
| | PDB: | 1BR2:A, 1BR2:B, 1BR2:C, 1BR2:D, 1BR2:E, 1BR2:F | | Structural/functional type: | Relationship to function unknown
| | Functional classes: | Unknown
| | Functional subclasses: | Unknown
| Detection methods:
- X-ray crystallography (100 K; pH: 6.8; cryoprotectant (25% polyethylene glydol 200))
| References:
- Dominguez R, Freyzon Y, Trybus KM, Cohen C. "Crystal structure of a vertebrate smooth muscle myosin motor domain and its complex with the essential light chain: visualization of the pre-power stroke state." Cell. 1998; 94(5): 559-71. PubMed: 9741621
| Comments:
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| Region 2 | | Type: | Disordered | | Name: | | | Location: | 50 - 55 | | Length: | 6 | | Region sequence: |
KEEKGD | | Modification type: | Engineered
| | PDB: | 1BR2:A, 1BR2:B, 1BR2:C, 1BR2:D, 1BR2:E, 1BR2:F | | Structural/functional type: | Relationship to function unknown
| | Functional classes: | Unknown
| | Functional subclasses: | Unknown
| Detection methods:
- X-ray crystallography (100 K; pH: 6.8; cryoprotectant (25% polyethylene glycol 200 ))
| References:
- Dominguez R, Freyzon Y, Trybus KM, Cohen C. "Crystal structure of a vertebrate smooth muscle myosin motor domain and its complex with the essential light chain: visualization of the pre-power stroke state." Cell. 1998; 94(5): 559-71. PubMed: 9741621
| Comments:
|
| Region 3 | | Type: | Disordered | | Name: | | | Location: | 200 - 217 | | Length: | 18 | | Region sequence: |
SHKGKKDTSITQGPSFSY | | Modification type: | Engineered
| | PDB: | 1BR2:A, 1BR2:B, 1BR2:C, 1BR2:D, 1BR2:E, 1BR2:F | | Structural/functional type: | Relationship to function unknown
| | Functional classes: | Unknown
| | Functional subclasses: | Unknown
| Detection methods:
- X-ray crystallography (100 K; pH: 6.8; cryoprotectant (25% polyethylene glycol 200 ))
| References:
- Rovner, A. S.
Freyzon, Y.
Trybus, K. M. "An insert in the motor domain determines the functional properties of expressed smooth muscle myosin isoforms." Journal of Muscle Research and Cell Motility. 1997; 18(1): 103-110. PubMed: 9147986
| Comments:The sequence information for this region came from Rovner et. al. (1997). It is a deviation from the Yanagisawa et. al sequence. This region is part of a 7 AA insert that helps to differentiate between fast and slow smooth muscle. The insert increases the protein’s enzymatic capabilities, as well as its ability to bind and move actin filaments.
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| Region 4 | | Type: | Disordered | | Name: | | | Location: | 371 - 374 | | Length: | 4 | | Region sequence: |
RNTD | | Modification type: | Engineered
| | PDB: | 1BR2:A, 1BR2:B, 1BR2:C, 1BR2:D, 1BR2:E, 1BR2:F | | Structural/functional type: | Relationship to function unknown
| | Functional classes: | Unknown
| | Functional subclasses: | Unknown
| Detection methods:
- X-ray crystallography (100 K; pH: 6.8; cryoprotectant (25% polyethylene glycol 200 ))
| References:
- Dominguez R, Freyzon Y, Trybus KM, Cohen C. "Crystal structure of a vertebrate smooth muscle myosin motor domain and its complex with the essential light chain: visualization of the pre-power stroke state." Cell. 1998; 94(5): 559-71. PubMed: 9741621
| Comments:
|
| Region 5 | | Type: | Disordered | | Name: | | | Location: | 406 - 415 | | Length: | 10 | | Region sequence: |
RIKVGRDVVQ | | Modification type: | Engineered
| | PDB: | 1BR2:A, 1BR2:B, 1BR2:C, 1BR2:D, 1BR2:E, 1BR2:F | | Structural/functional type: | Relationship to function unknown
| | Functional classes: | Unknown
| | Functional subclasses: | Unknown
| Detection methods:
- X-ray crystallography (100 K; pH: 6.8; cryoprotectant (25% polyethylene glycol 200 ))
| References:
- Dominguez R, Freyzon Y, Trybus KM, Cohen C. "Crystal structure of a vertebrate smooth muscle myosin motor domain and its complex with the essential light chain: visualization of the pre-power stroke state." Cell. 1998; 94(5): 559-71. PubMed: 9741621
| Comments:
|
| Region 6 | | Type: | Disordered | | Name: | | | Location: | 452 - 457 | | Length: | 6 | | Region sequence: |
KTKRQG | | Modification type: | Engineered
| | PDB: | 1BR2:A, 1BR2:B, 1BR2:C, 1BR2:D, 1BR2:E, 1BR2:F | | Structural/functional type: | Relationship to function unknown
| | Functional classes: | Unknown
| | Functional subclasses: | Unknown
| Detection methods:
- X-ray crystallography (100 K; pH: 6.8; cryoprotectant (25% polyethylene glycol 200 ))
| References:
- Dominguez R, Freyzon Y, Trybus KM, Cohen C. "Crystal structure of a vertebrate smooth muscle myosin motor domain and its complex with the essential light chain: visualization of the pre-power stroke state." Cell. 1998; 94(5): 559-71. PubMed: 9741621
| Comments:
|
| Region 7 | | Type: | Disordered | | Name: | | | Location: | 546 - 549 | | Length: | 4 | | Region sequence: |
WFPK | | Modification type: | Engineered
| | PDB: | 1BR2:A, 1BR2:B, 1BR2:C, 1BR2:D, 1BR2:E, 1BR2:F | | Structural/functional type: | Relationship to function unknown
| | Functional classes: | Unknown
| | Functional subclasses: | Unknown
| Detection methods:
- X-ray crystallography (100 K; pH: 6.8; cryoprotectant (25% polyethylene glycol 200 ))
| References:
- Dominguez R, Freyzon Y, Trybus KM, Cohen C. "Crystal structure of a vertebrate smooth muscle myosin motor domain and its complex with the essential light chain: visualization of the pre-power stroke state." Cell. 1998; 94(5): 559-71. PubMed: 9741621
| Comments:The PDB file 1BR2 shows the disordered region ending at amino acid PRO-548.
|
| Region 8 | | Type: | Disordered | | Name: | | | Location: | 573 - 577 | | Length: | 5 | | Region sequence: |
KQLKD | | Modification type: | Engineered
| | PDB: | 1BR2:A, 1BR2:B, 1BR2:C, 1BR2:D, 1BR2:E, 1BR2:F | | Structural/functional type: | Relationship to function unknown
| | Functional classes: | Unknown
| | Functional subclasses: | Unknown
| Detection methods:
- X-ray crystallography (100 K; pH: 6.8; cryoprotectant (25% polyethylene glycol 200 ))
| References:
- Dominguez R, Freyzon Y, Trybus KM, Cohen C. "Crystal structure of a vertebrate smooth muscle myosin motor domain and its complex with the essential light chain: visualization of the pre-power stroke state." Cell. 1998; 94(5): 559-71. PubMed: 9741621
| Comments:
|
| Region 9 | | Type: | Disordered | | Name: | | | Location: | 627 - 656 | | Length: | 30 | | Region sequence: |
DVDRIVGLDQMAKMTESSLPSASKTKKGMF | | Modification type: | Engineered
| | PDB: | 1BR2:A, 1BR2:B, 1BR2:C, 1BR2:D, 1BR2:E, 1BR2:F | | Structural/functional type: | Relationship to function unknown
| | Functional classes: | Unknown
| | Functional subclasses: | Unknown
| Detection methods:
- X-ray crystallography (100 K; pH: 6.8; cryoprotectant (25% polyethylene glycol 200 ))
| References:
- Dominguez R, Freyzon Y, Trybus KM, Cohen C. "Crystal structure of a vertebrate smooth muscle myosin motor domain and its complex with the essential light chain: visualization of the pre-power stroke state." Cell. 1998; 94(5): 559-71. PubMed: 9741621
| Comments:
|
| Region 10 | | Type: | Disordered | | Name: | | | Location: | 790 - 791 | | Length: | 2 | | Region sequence: |
LK | | Modification type: | Engineered
| | PDB: | 1BR2:A, 1BR2:B, 1BR2:C, 1BR2:D, 1BR2:E, 1BR2:F | | Structural/functional type: | Relationship to function unknown
| | Functional classes: | Unknown
| | Functional subclasses: | Unknown
| Detection methods:
- X-ray crystallography (100 K; pH: 6.8; cryoprotectant (25% polyethylene glycol 200 ))
| References:
- Dominguez R, Freyzon Y, Trybus KM, Cohen C. "Crystal structure of a vertebrate smooth muscle myosin motor domain and its complex with the essential light chain: visualization of the pre-power stroke state." Cell. 1998; 94(5): 559-71. PubMed: 9741621
| Comments:
|
| References |
- Rovner, A. S.
Freyzon, Y.
Trybus, K. M. "An insert in the motor domain determines the functional properties of expressed smooth muscle myosin isoforms." Journal of Muscle Research and Cell Motility. 1997; 18(1): 103-110. PubMed: 9147986
- Yanagisawa M, Hamada Y, Katsuragawa Y, Imamura M, Mikawa T, Masaki T. "Complete primary structure of vertebrate smooth muscle myosin heavy chain deduced from its complementary DNA sequence. Implications on topography and function of myosin." J Mol Biol. 1987; 198(2): 143-57. PubMed: 2892941
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