General information | DisProt: | DP00111 | Name: | Protein C-ets-1 | Synonym(s): | ETS1_MOUSE
p54
| First appeared in release: | Release 3.0 (02/17/2006) | UniProt: | P27577 | UniGene: | Mm.292415 | SwissProt: | ETS1_MOUSE | TrEMBL: | | NCBI (GI): | 729453 | Source organism: | Mus musculus (Mouse) | Sequence length: | 440 | Percent disordered: | 24% | Homologues: | |
Native sequence |
10 20 30 40 50 60 | | | | | | MKAAVDLKPT LTIIKTEKVD LELFPSPDME CADVPLLTPS SKEMMSQALK ATFSGFTKEQ - 60 QRLGIPKDPR QWTETHVRDW VMWAVNEFSL KGVDFQKFCM SGAALCALGK ECFLELAPDF - 120 VGDILWEHLE ILQKEDVKPY QVNGANPTYP ESCYTSDYFI SYGIEHAQCV PPSEFSEPSF - 180 ITESYQTLHP ISSEELLSLK YENDYPSVIL QDPLQTDTLQ TDYFAIKQEV LTPDNMCLGR - 240 ASRGKLGGQD SFESVESYDS CDRLTQSWSS QSSFNSLQRV PSYDSFDYED YPAALPNHKP - 300 KGTFKDYVRD RADLNKDKPV IPAAALAGYT GSGPIQLWQF LLELLTDKSC QSFISWTGDG - 360 WEFKLSDPDE VARRWGKRKN KPKMNYEKLS RGLRYYYDKN IIHKTAGKRY VYRFVCDLQS - 420 LLGYTPEELH AMLDVKPDAD
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Functional narrative |
Ets-1 is the founding member of a family of winged helix-turn-helix transcription factors that share homologies with the v-ets sequence of the E26 avian leukemia virus. The ETS family of transcription factors is characterized by a conserved ETS domain that is capable of binding to DNA sequences containing a core GGAA/T motif. In a variety of species, ETS proteins are involved in the regulation of developmental processes in response to extracellular signals. For instance, activation of the Ras pathway modulates the activities of Ets-1 through an extracellular signal-regulated kinase-mediated phosphorylation on a threonine residue. Further regulations are triggered by the calcium/calmodulin-dependent protein kinase II, through phosphorylations of serine residues located near the Ets-1 DNA-binding domain. Interestingly, both of these regulatory pathways have been shown to target Ets-1 after lymphocyte activation. Gene targeting experiments in mice have established that Ets-1 is essential for the development of both the natural killer and T cell lineages. Studies on Ets-1–deficient (Ets-1–/–) mice have demonstrated that Ets-1 is important for T cell development, proliferation, and survival. In addition, Ets-1 has been shown to regulate several cytokine gene promoters and might also modulate the effector function of Th cells.
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Map of ordered and disordered regions |
Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.
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Region 1 | Type: | Disordered | Name: | N-terminal region | Location: | 1 - 49 | Length: | 49 | Region sequence: |
MKAAVDLKPTLTIIKTEKVDLELFPSPDMECADVPLLTPSSKEMMSQAL | Modification type: | Fragment
Native
| PDB: | 1BQV:A | Structural/functional type: | | Functional classes: | | Functional subclasses: | | Detection methods:
- Nuclear magnetic resonance (NMR) (303 K; pH: 6.5; 10% D2O; 10 mM KCl, 10 mM KH2PO4, 10 mM DTT; 1.2 mM Ets-1; amide 1HN and 15N chemical shifts)
- Sensitivity to proteolysis (296 K; pH: 6; 200 ng trypsin; 20mMcitrate, 150mMKCl, 1mMDTT; 2.75 microg protein)
| References:
- Slupsky CM, Gentile LN, Donaldson LW, Mackereth CD, Seidel JJ, Graves BJ, McIntosh LP. "Structure of the Ets-1 pointed domain and mitogen-activated protein kinase phosphorylation site." Proc Natl Acad Sci U S A. 1998; 95(21): 12129-34. PubMed: 9770451
| Comments:
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Region 2 | Type: | Disordered | Name: | SRR | Location: | 244 - 301 | Length: | 58 | Region sequence: |
GKLGGQDSFESVESYDSCDRLTQSWSSQSSFNSLQRVPSYDSFDYEDYPAALPNHKPK | Modification type: | Fragment
| PDB: | | Structural/functional type: | Function arises via a disorder to order transition | Functional classes: | Molecular assembly
| Functional subclasses: | Phosphorylation
Protein-DNA binding
Autoregulatory
| Detection methods:
- Nuclear magnetic resonance (NMR)
| References:
- Pufall MA, Lee GM, Nelson ML, Kang HS, Velyvis A, Kay LE, McIntosh LP, Graves BJ. "Variable control of Ets-1 DNA binding by multiple phosphates in an unstructured region." Science. 2005; 309(5731): 142-5. PubMed: 15994560
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References |
- Tymms MJ, Kola I. "Regulation of gene expression by transcription factors Ets-1 and Ets-2." Mol Reprod Dev. 1994; 39(2): 208-14. PubMed: 7826624
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