DP00111: Protein C-ets-1FASTA viewXML view

General information
DisProt:DP00111
Name:Protein C-ets-1
Synonym(s):ETS1_MOUSE
p54
First appeared in release:Release 3.0 (02/17/2006)
UniProt:P27577
UniGene:Mm.292415
SwissProt: ETS1_MOUSE
TrEMBL:  
NCBI (GI): 729453
Source organism:Mus musculus (Mouse)
Sequence length:440
Percent disordered:24%
Homologues: 


Native sequence

        10         20         30         40         50         60
         |          |          |          |          |          |
MKAAVDLKPT LTIIKTEKVD LELFPSPDME CADVPLLTPS SKEMMSQALK ATFSGFTKEQ - 60
QRLGIPKDPR QWTETHVRDW VMWAVNEFSL KGVDFQKFCM SGAALCALGK ECFLELAPDF - 120
VGDILWEHLE ILQKEDVKPY QVNGANPTYP ESCYTSDYFI SYGIEHAQCV PPSEFSEPSF - 180
ITESYQTLHP ISSEELLSLK YENDYPSVIL QDPLQTDTLQ TDYFAIKQEV LTPDNMCLGR - 240
ASRGKLGGQD SFESVESYDS CDRLTQSWSS QSSFNSLQRV PSYDSFDYED YPAALPNHKP - 300
KGTFKDYVRD RADLNKDKPV IPAAALAGYT GSGPIQLWQF LLELLTDKSC QSFISWTGDG - 360
WEFKLSDPDE VARRWGKRKN KPKMNYEKLS RGLRYYYDKN IIHKTAGKRY VYRFVCDLQS - 420
LLGYTPEELH AMLDVKPDAD



Functional narrative    

Ets-1 is the founding member of a family of winged helix-turn-helix transcription factors that share homologies with the v-ets sequence of the E26 avian leukemia virus. The ETS family of transcription factors is characterized by a conserved ETS domain that is capable of binding to DNA sequences containing a core GGAA/T motif. In a variety of species, ETS proteins are involved in the regulation of developmental processes in response to extracellular signals. For instance, activation of the Ras pathway modulates the activities of Ets-1 through an extracellular signal-regulated kinase-mediated phosphorylation on a threonine residue. Further regulations are triggered by the calcium/calmodulin-dependent protein kinase II, through phosphorylations of serine residues located near the Ets-1 DNA-binding domain. Interestingly, both of these regulatory pathways have been shown to target Ets-1 after lymphocyte activation. Gene targeting experiments in mice have established that Ets-1 is essential for the development of both the natural killer and T cell lineages. Studies on Ets-1–deficient (Ets-1–/–) mice have demonstrated that Ets-1 is important for T cell development, proliferation, and survival. In addition, Ets-1 has been shown to regulate several cytokine gene promoters and might also modulate the effector function of Th cells.

Region 1: 1-49 Region 2: 244-301

Map of ordered and disordered regions







Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.


Region 1
Type:Disordered
Name:N-terminal region
Location:1 - 49
Length:49
Region sequence:

MKAAVDLKPTLTIIKTEKVDLELFPSPDMECADVPLLTPSSKEMMSQAL

Modification type: Fragment
Native
PDB: 1BQV:A
Structural/functional type:  
Functional classes:  
Functional subclasses:  
Detection methods:
  1. Nuclear magnetic resonance (NMR) (303 K; pH: 6.5; 10% D2O; 10 mM KCl, 10 mM KH2PO4, 10 mM DTT; 1.2 mM Ets-1; amide 1HN and 15N chemical shifts)

  2. Sensitivity to proteolysis (296 K; pH: 6; 200 ng trypsin; 20mMcitrate, 150mMKCl, 1mMDTT; 2.75 microg protein)

References:
  1. Slupsky CM, Gentile LN, Donaldson LW, Mackereth CD, Seidel JJ, Graves BJ, McIntosh LP. "Structure of the Ets-1 pointed domain and mitogen-activated protein kinase phosphorylation site." Proc Natl Acad Sci U S A. 1998; 95(21): 12129-34. PubMed: 9770451

Comments:
 



Region 2
Type:Disordered
Name:SRR
Location:244 - 301
Length:58
Region sequence:

GKLGGQDSFESVESYDSCDRLTQSWSSQSSFNSLQRVPSYDSFDYEDYPAALPNHKPK

Modification type: Fragment
PDB:  
Structural/functional type: Function arises via a disorder to order transition
Functional classes: Molecular assembly
Functional subclasses: Phosphorylation
Protein-DNA binding
Autoregulatory
Detection methods:
  1. Nuclear magnetic resonance (NMR)

References:
  1. Pufall MA, Lee GM, Nelson ML, Kang HS, Velyvis A, Kay LE, McIntosh LP, Graves BJ. "Variable control of Ets-1 DNA binding by multiple phosphates in an unstructured region." Science. 2005; 309(5731): 142-5. PubMed: 15994560

Comments:
 



References

  1. Tymms MJ, Kola I. "Regulation of gene expression by transcription factors Ets-1 and Ets-2." Mol Reprod Dev. 1994; 39(2): 208-14. PubMed: 7826624


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