DP00117: Protein transport protein Sec61 subunit gammaFASTA viewXML view

General information
DisProt:DP00117
Name:Protein transport protein Sec61 subunit gamma
Synonym(s):SC61G_CANFA
First appeared in release:Release 1.0 (08/01/2003)
UniProt:P60058
UniGene:Cfa.40294
SwissProt: SC61G_CANFA
TrEMBL:  
NCBI (GI): 38503385
Source organism:Canis familiaris (dog)
Sequence length:68
Percent disordered:51%
Homologues: 


Native sequence

        10         20         30         40         50         60
         |          |          |          |          |          |
MDQVMQFVEP SRQFVKDSIR LVKRCTKPDR KEFQKIAMAT AIGFAIMGFI GFFVKLIHIP - 60
INNIIVGG



Functional narrative    

The gamma subunit of Sec61, a member of the SecE/Sec61gamma family, is an essential member of a complex which assists in the translocation of proteins in the endoplasmic reticulum. Sec61gamma is bound by its tail to the membrane of the cell. It interacts directly with Sec61-alpha and Sec61-beta and also indirectly with many other secretory proteins. This protein was characterized via the analysis of a synthesized fragment containing amino acid residues 1 - 43. A point mutation, Cys24Ser, was also included in the synthesis of this fragment. The mutation was introduced in order to prevent dimerization. The disordered regions in Sec61 gamma are associated with membrane binding.

Region 3: 1-4 Region 1: 1-9 Region 2: 18-43 Region 4: 40-43

Map of ordered and disordered regions







Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.


Region 1
Type:Disordered - Extended
Name: 
Location:1 - 9
Length:9
Region sequence:

MDQVMQFVE

Modification type: Engineered
Fragment
Mutant
PDB:  
Structural/functional type: Relationship to function unknown
Functional classes: Unknown
Functional subclasses: Unknown
Detection methods:
  1. Nuclear magnetic resonance (NMR) (274 K; pH: 5; )

References:
  1. Beswick V, Baleux F, Huynh-Dinh T, Kepes F, Neumann JM, Sanson A. "NMR conformational study of the cytoplasmic domain of the canine Sec61 gamma protein from the protein translocation pore of the endoplasmic reticulum membrane." Biochemistry. 1996; 35(47): 14717-24. PubMed: 8942632

Comments:
This disordered region was characterized by the study of an engineered fragment containing residues 1-43. This fragment was synthesized via the Merrifield solid-phase method with a mutation at Cys24Ser. This mutation was inserted to prevent dimerization.




Region 2
Type:Disordered - Extended
Name: 
Location:18 - 43
Length:26
Region sequence:

SIRLVKRCTKPDRKEFQKIAMATAIG

Modification type: Engineered
Fragment
Mutant
PDB:  
Structural/functional type: Relationship to function unknown
Functional classes: Unknown
Functional subclasses: Unknown
Detection methods:
  1. Nuclear magnetic resonance (NMR) (274 K; pH: 5; )

References:
  1. Beswick V, Baleux F, Huynh-Dinh T, Kepes F, Neumann JM, Sanson A. "NMR conformational study of the cytoplasmic domain of the canine Sec61 gamma protein from the protein translocation pore of the endoplasmic reticulum membrane." Biochemistry. 1996; 35(47): 14717-24. PubMed: 8942632

Comments:
This disordered region was characterized by the study of an engineered fragment containing residues 1-43. This fragment was synthesized via the Merrifield solid-phase method with a mutation at Cys24Ser. This mutation was inserted to prevent dimerization.




Region 3
Type:Disordered - Extended
Name: 
Location:1 - 4
Length:4
Region sequence:

MDQV

Modification type: Engineered
Fragment
Mutant
PDB:  
Structural/functional type: Relationship to function unknown
Functional classes: Unknown
Functional subclasses: Unknown
Detection methods:
  1. Nuclear magnetic resonance (NMR) (311 K; pH: 6; perdeuterated DPC )

References:
  1. Beswick V, Baleux F, Huynh-Dinh T, Kepes F, Neumann JM, Sanson A. "NMR conformational study of the cytoplasmic domain of the canine Sec61 gamma protein from the protein translocation pore of the endoplasmic reticulum membrane." Biochemistry. 1996; 35(47): 14717-24. PubMed: 8942632

Comments:
This disordered region was characterized by the study of an engineered fragment containing residues 1-43. This fragment was synthesized via the Merrifield solid-phase method with a mutation at Cys24Ser. This mutation was inserted to prevent dimerization.




Region 4
Type:Disordered - Extended
Name: 
Location:40 - 43
Length:4
Region sequence:

TAIG

Modification type: Engineered
Fragment
Mutant
PDB:  
Structural/functional type: Relationship to function unknown
Functional classes: Unknown
Functional subclasses: Unknown
Detection methods:
  1. Nuclear magnetic resonance (NMR) (311 K; pH: 5; perdeuterated DPC )

References:
  1. Beswick V, Baleux F, Huynh-Dinh T, Kepes F, Neumann JM, Sanson A. "NMR conformational study of the cytoplasmic domain of the canine Sec61 gamma protein from the protein translocation pore of the endoplasmic reticulum membrane." Biochemistry. 1996; 35(47): 14717-24. PubMed: 8942632

Comments:
This disordered region was characterized by the study of an engineered fragment containing residues 1-43. This fragment was synthesized via the Merrifield solid-phase method with a mutation at Cys24Ser. This mutation was inserted to prevent dimerization.



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