| General information | | DisProt: | DP00130 | | Name: | Small proline-rich protein 2E | | Synonym(s): | SPR2E_HUMAN
SPR-2E
Small proline-rich protein II
SPR-II
| | First appeared in release: | Release 2.1 (03/14/2005) | | UniProt: | P22531 | | UniGene: | Hs.568518 | | SwissProt: | SPR2E_HUMAN | | TrEMBL: | | | NCBI (GI): | 45644952 | | Source organism: | Homo sapiens (Human) | | Sequence length: | 72 | | Percent disordered: | 63% | | Homologues: | |
| Native sequence |
10 20 30 40 50 60
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MSYQQQQCKQ PCQPPPVCPT PKCPEPCPPP KCPEPCPPPK CPQPSPPQQC QQKCPPVTPS - 60
PPCQPKCPPK SK
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| Functional narrative |
Small proline-rich 2 protein 2E is a member of the cornifin (SPRR) family of proteins which are associated with the cornified cell envelope of squamous cells of the skin. This protein can be located in the cytoplasm until it interacts directly with TGase 1, 2, or 3 at which point it can be found bound the cell membrane.
Both the C and N terminus of this protein are disordered when the protein is in the cytoplasm. These disordered regions then undergo a disorder to order transition to interact with TGase 1, 2, or 3.
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| Map of ordered and disordered regions |
Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.
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| Region 1 | | Type: | Disordered - Extended | | Name: | | | Location: | 1 - 20 | | Length: | 20 | | Region sequence: |
MSYQQQQCKQPCQPPPVCPT | | Modification type: | Native
| | PDB: | | | Structural/functional type: | Function arises via a disorder to order transition
| | Functional classes: | Molecular assembly
| | Functional subclasses: | Protein-protein binding
| Detection methods:
- SDS-PAGE gel, Aberrant mobility on (310 K; TGase 1 or 3; trypsin)
- Circular dichroism (CD) spectroscopy, near-UV (Phosphate Buffer)
- Sensitivity to proteolysis (310 K; TGase 1 or 3; trypsin)
- Stability at thermal extremes (293 K; )
| References:
- Kartasova T, van de Putte P. "Isolation, characterization, and UV-stimulated expression of two families of genes encoding polypeptides of related structure in human epidermal keratinocytes." Mol Cell Biol. 1988; 8(5): 2195-2203. PubMed: 3133554
- Tarcsa E, Candi E, Kartasova T, Idler WW, Marekov LN, Steinert PM. "Structural and transglutaminase substrate properties of the small proline-rich 2 family of cornified cell envelope proteins." J Biol Chem. 1998; 273(36): 23297-23303. PubMed: 9722562
| Comments:
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| Region 2 | | Type: | Disordered - Extended | | Name: | | | Location: | 48 - 72 | | Length: | 25 | | Region sequence: |
QQCQQKCPPVTPSPPCQPKCPPKSK | | Modification type: | Native
| | PDB: | | | Structural/functional type: | Function arises via a disorder to order transition
| | Functional classes: | Molecular assembly
| | Functional subclasses: | Protein-protein binding
| Detection methods:
- SDS-PAGE gel, Aberrant mobility on (310 K; TGase 1 or 3; trypsin)
- Circular dichroism (CD) spectroscopy, near-UV (293 K; Phosphate Buffer)
- Sensitivity to proteolysis (310 K; TGase 1 or 2; trypsin)
- Stability at thermal extremes (293 K; )
| References:
- Kartasova T, van de Putte P. "Isolation, characterization, and UV-stimulated expression of two families of genes encoding polypeptides of related structure in human epidermal keratinocytes." Mol Cell Biol. 1988; 8(5): 2195-2203. PubMed: 3133554
- Tarcsa E, Candi E, Kartasova T, Idler WW, Marekov LN, Steinert PM. "Structural and transglutaminase substrate properties of the small proline-rich 2 family of cornified cell envelope proteins." J Biol Chem. 1998; 273(36): 23297-23303. PubMed: 9722562
| Comments:
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