DP00135: Heat shock factor proteinFASTA viewXML view

General information
DisProt:DP00135
Name:Heat shock factor protein
Synonym(s):HSF_YEAST
HSF
Heat shock transcription factor
HSTF
First appeared in release:Release 3.0 (02/17/2006)
UniProt:P10961
UniGene: 
SwissProt: HSF_YEAST
TrEMBL:  
NCBI (GI): 123687
Source organism:Saccharomyces cerevisiae (Baker's yeast)
Sequence length:833
Percent disordered:20%
Homologues: 


Native sequence

        10         20         30         40         50         60
         |          |          |          |          |          |
MNNAANTGTT NESNVSDAPR IEPLPSLNDD DIEKILQPND IFTTDRTDAS TTSSTAIEDI - 60
INPSLDPQSA ASPVPSSSFF HDSRKPSTST HLVRRGTPLG IYQTNLYGHN SRENTNPNST - 120
LLSSKLLAHP PVPYGQNPDL LQHAVYRAQP SSGTTNAQPR QTTRRYQSHK SRPAFVNKLW - 180
SMLNDDSNTK LIQWAEDGKS FIVTNREEFV HQILPKYFKH SNFASFVRQL NMYGWHKVQD - 240
VKSGSIQSSS DDKWQFENEN FIRGREDLLE KIIRQKGSSN NHNSPSGNGN PANGSNIPLD - 300
NAAGSNNSNN NISSSNSFFN NGHLLQGKTL RLMNEANLGD KNDVTAILGE LEQIKYNQIA - 360
ISKDLLRINK DNELLWQENM MARERHRTQQ QALEKMFRFL TSIVPHLDPK MIMDGLGDPK - 420
VNNEKLNSAN NIGLNRDNTG TIDELKSNDS FINDDRNSFT NATTNARNNM SPNNDDNSID - 480
TASTNTTNRK KNIDENIKNN NDIINDIIFN TNLANNLSNY NSNNNAGSPI RPYKQRYLLK - 540
NRANSSTSSE NPSLTPFDIE SNNDRKISEI PFDDEEEEET DFRPFTSRDP NNQTSENTFD - 600
PNRFTMLSDD DLKKDSHTND NKHNESDLFW DNVHRNIDEQ DARLQNLENM VHILSPGYPN - 660
KSFNNKTSST NTNSNMESAV NVNSPGFNLQ DYLTGESNSP NSVHSVPSNG SGSTPLPMPN - 720
DNDTEHASTS VNQGENGSGL TPFLTVDDHT LNDNNTSEGS TRVSPDIKFS ATENTKVSDN - 780
LPSFNDHSYS TQADTAPENA KKRFVEEIPE PAIVEIQDPT EYNDHRLPKR AKK



Functional narrative    

DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. Also required for growth at normal temperatures. Transcription factors orchestrate the regulated production of key proteins in eukaryotic cells during development and in response to extracellular stimuli. Typical transcription factors consist of a DNA-binding domain, an oligomerization domain, and one or more activation domain.

Region 1: 1-167

Map of ordered and disordered regions







Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.


Region 1
Type:Disordered
Name:N-terminal activation domain
Location:1 - 167
Length:167
Region sequence:

MNNAANTGTTNESNVSDAPRIEPLPSLNDDDIEKILQPNDIFTTDRTDASTTSSTAIEDI
INPSLDPQSAASPVPSSSFFHDSRKPSTSTHLVRRGTPLGIYQTNLYGHNSRENTNPNST
LLSSKLLAHPPVPYGQNPDLLQHAVYRAQPSSGTTNAQPRQTTRRYQ

Modification type: Native
PDB:  
Structural/functional type: Function arises from the disordered state
Functional classes: Modification site
Functional subclasses: Transactivation (transcriptional activation)
Detection methods:
  1. Nuclear magnetic resonance (NMR) (heteronuclear NOE spectrum; pH 3.4 and 5.75)

  2. Circular dichroism (CD) spectroscopy, far-UV (298 K; pH: 7; 1 mg/mL protein solutions in 25 mM sodium phosphate buffer)

References:
  1. Cho HS, Liu CW, Damberger FF, Pelton JG, Nelson HC, Wemmer DE. "Yeast heat shock transcription factor N-terminal activation domains are unstructured as probed by heteronuclear NMR spectroscopy." Protein Sci. 1996; 5(2): 262-9. PubMed: 8745404

Comments:
 



References

  1. Imazu H, Sakurai H. "Saccharomyces cerevisiae heat shock transcription factor regulates cell wall remodeling in response to heat shock." Eukaryot Cell. 2005; 4(6): 1050-6. PubMed: 15947197


If you have any comments or wish to provide additional references to this protein or its disordered region(s), please click here to e-mail us.


Disprot-footer
Contact us