General information | DisProt: | DP00135 | Name: | Heat shock factor protein | Synonym(s): | HSF_YEAST
HSF
Heat shock transcription factor
HSTF
| First appeared in release: | Release 3.0 (02/17/2006) | UniProt: | P10961 | UniGene: | | SwissProt: | HSF_YEAST | TrEMBL: | | NCBI (GI): | 123687 | Source organism: | Saccharomyces cerevisiae (Baker's yeast) | Sequence length: | 833 | Percent disordered: | 20% | Homologues: | |
Native sequence |
10 20 30 40 50 60 | | | | | | MNNAANTGTT NESNVSDAPR IEPLPSLNDD DIEKILQPND IFTTDRTDAS TTSSTAIEDI - 60 INPSLDPQSA ASPVPSSSFF HDSRKPSTST HLVRRGTPLG IYQTNLYGHN SRENTNPNST - 120 LLSSKLLAHP PVPYGQNPDL LQHAVYRAQP SSGTTNAQPR QTTRRYQSHK SRPAFVNKLW - 180 SMLNDDSNTK LIQWAEDGKS FIVTNREEFV HQILPKYFKH SNFASFVRQL NMYGWHKVQD - 240 VKSGSIQSSS DDKWQFENEN FIRGREDLLE KIIRQKGSSN NHNSPSGNGN PANGSNIPLD - 300 NAAGSNNSNN NISSSNSFFN NGHLLQGKTL RLMNEANLGD KNDVTAILGE LEQIKYNQIA - 360 ISKDLLRINK DNELLWQENM MARERHRTQQ QALEKMFRFL TSIVPHLDPK MIMDGLGDPK - 420 VNNEKLNSAN NIGLNRDNTG TIDELKSNDS FINDDRNSFT NATTNARNNM SPNNDDNSID - 480 TASTNTTNRK KNIDENIKNN NDIINDIIFN TNLANNLSNY NSNNNAGSPI RPYKQRYLLK - 540 NRANSSTSSE NPSLTPFDIE SNNDRKISEI PFDDEEEEET DFRPFTSRDP NNQTSENTFD - 600 PNRFTMLSDD DLKKDSHTND NKHNESDLFW DNVHRNIDEQ DARLQNLENM VHILSPGYPN - 660 KSFNNKTSST NTNSNMESAV NVNSPGFNLQ DYLTGESNSP NSVHSVPSNG SGSTPLPMPN - 720 DNDTEHASTS VNQGENGSGL TPFLTVDDHT LNDNNTSEGS TRVSPDIKFS ATENTKVSDN - 780 LPSFNDHSYS TQADTAPENA KKRFVEEIPE PAIVEIQDPT EYNDHRLPKR AKK
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Functional narrative |
DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. Also required for growth at normal temperatures. Transcription factors orchestrate the regulated production of
key proteins in eukaryotic cells during development and in response
to extracellular stimuli. Typical transcription factors consist
of a DNA-binding domain, an oligomerization domain, and
one or more activation domain.
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Map of ordered and disordered regions |
Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.
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Region 1 | Type: | Disordered | Name: | N-terminal activation domain | Location: | 1 - 167 | Length: | 167 | Region sequence: |
MNNAANTGTTNESNVSDAPRIEPLPSLNDDDIEKILQPNDIFTTDRTDASTTSSTAIEDI INPSLDPQSAASPVPSSSFFHDSRKPSTSTHLVRRGTPLGIYQTNLYGHNSRENTNPNST LLSSKLLAHPPVPYGQNPDLLQHAVYRAQPSSGTTNAQPRQTTRRYQ | Modification type: | Native
| PDB: | | Structural/functional type: | Function arises from the disordered state | Functional classes: | Modification site
| Functional subclasses: | Transactivation (transcriptional activation)
| Detection methods:
- Nuclear magnetic resonance (NMR) (heteronuclear NOE spectrum; pH 3.4 and 5.75)
- Circular dichroism (CD) spectroscopy, far-UV (298 K; pH: 7; 1 mg/mL protein solutions in 25 mM sodium phosphate buffer)
| References:
- Cho HS, Liu CW, Damberger FF, Pelton JG, Nelson HC, Wemmer DE. "Yeast heat shock transcription factor N-terminal activation domains are unstructured as probed by heteronuclear NMR spectroscopy." Protein Sci. 1996; 5(2): 262-9. PubMed: 8745404
| Comments:
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References |
- Imazu H, Sakurai H. "Saccharomyces cerevisiae heat shock transcription factor regulates cell wall remodeling in response to heat shock." Eukaryot Cell. 2005; 4(6): 1050-6. PubMed: 15947197
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