| General information | | DisProt: | DP00140 | | Name: | 50S ribosomal protein L27 | | Synonym(s): | RL27_ECOLI
| | First appeared in release: | Release 1.0 (08/01/2003) | | UniProt: | P0A7L8 | | UniGene: | | | SwissProt: | RL27_ECOLI | | TrEMBL: | | | NCBI (GI): | 67472010 | | Source organism: | Escherichia coli | | Sequence length: | 85 | | Percent disordered: | 100% | | Homologues: | |
| Native sequence |
10 20 30 40 50 60
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MAHKKAGGST RNGRDSEAKR LGVKRFGGES VLAGSIIVRQ RGTKFHAGAN VGCGRDHTLF - 60
AKADGKVKFE VKGPKNRKFI SIEAE
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| Functional narrative |
This protein is one of many that combine to form the 50S ribosomal subunit. The 50S ribosomal subunit has difficulty forming if the L27 is missing.
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| Map of ordered and disordered regions |
Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.
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| Region 1 | | Type: | Disordered | | Name: | | | Location: | 1 - 85 | | Length: | 85 | | Region sequence: |
MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTKFHAGANVGCGRDHTLF
AKADGKVKFEVKGPKNRKFISIEAE | | Modification type: | Native
| | PDB: | | | Structural/functional type: | Relationship to function unknown
| | Functional classes: | Molecular assembly
| | Functional subclasses: | Protein-protein binding
| Detection methods:
- Circular dichroism (CD) spectroscopy, near-UV (277 K; pH: 7; beta-mercaptoethanol 1 mM; sodium phosphate 1 mM)
- Nuclear magnetic resonance (NMR) (293 K; pH: 7; 2D heteronuclear NMR (M9 medium with 15NH4SO4 supplement); beta-mercaptoethanol 1 mM; sodium phosphate 1 mM; Tetramethylsilane phosphate (as internal chemical shift standard))
- Nuclear magnetic resonance (NMR) (353 K; pH: 7; 2D heteronuclear NMR (M9 medium with 15NH4SO4 supplement); beta-mercaptoethanol 1 mM; sodium phosphate 1 mM; Tetramethylsilane phosphate (as internal chemical shift standard))
| References:
- Littlechild J, Malcolm A, Paterakis K, Ackermann I, Dijk J. "The tertiary structure of salt-extracted ribosomal proteins from Escherichia coli as studied by proton magnetic resonance spectroscopy and limited proteolysis experiments." Biochim Biophys Acta. 1987; 913(2): 245-55. PubMed: 3297162
- Maguire BA, Manuilov AV, Zimmermann RA. "Differential effects of replacing Escherichia coli ribosomal protein L27 with its homologue from Aquifex aeolicus." J Bacteriol. 2001; 183(22): 6565-72. PubMed: 11673426
| Comments:
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