DP00155: Syntaxin-1AFASTA viewXML view

General information
DisProt:DP00155
Name:Syntaxin-1A
Synonym(s):STX1A_RAT
Synaptotagmin-associated 35 kDa protein
P35A
Neuron-specific antigen HPC-1
First appeared in release:Release 2.0 (02/14/2005)
UniProt:P32851
UniGene:Rn.9943
SwissProt: STX1A_RAT
TrEMBL:  
NCBI (GI): 417842
Source organism:Rattus norvegicus (Rat)
Sequence length:288
Percent disordered:12%
Homologues: 


Native sequence

        10         20         30         40         50         60
         |          |          |          |          |          |
MKDRTQELRT AKDSDDDDDV TVTVDRDRFM DEFFEQVEEI RGFIDKIAEN VEEVKRKHSA - 60
ILASPNPDEK TKEELEELMS DIKKTANKVR SKLKSIEQSI EQEEGLNRSS ADLRIRKTQH - 120
STLSRKFVEV MSEYNATQSD YRERCKGRIQ RQLEITGRTT TSEELEDMLE SGNPAIFASG - 180
IIMDSSISKQ ALSEIETRHS EIIKLENSIR ELHDMFMDMA MLVESQGEMI DRIEYNVEHA - 240
VDYVERAVSD TKKAVKYQSK ARRKKIMIII CCVILGIIIA STIGGIFG



Functional narrative    

Syntaxin 1A is involved in membrane fusion and exocytosis. This membrane-bound member of the SNARE family interacts directly with SNAP25 and synaptobrevin (VAMP) to form the SNARE complex. This complex can then interact with ATPase NSF and indirectly with SNAPs to separate into its individual pieces. Syntaxin 1A can also bind with munc18, munc13, and their homologs to form stable complexes which regulate the formation of the SNARE complex. In the presence of munc18, the disordered region binds to munc18 causing the disordered region to alter its conformation. This change assists in the regulation of the SNARE complex. If mutations occur at L165A and E166A, in the linker region, munc18 will not bind to Syntaxin 1A (Margittai 2003).

Region 1: 149-182

Map of ordered and disordered regions







Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.


Region 1
Type:Disordered - Extended
Name: 
Location:149 - 182
Length:34
Region sequence:

IQRQLEITGRTTTSEELEDMLESGNPAIFASGII

Modification type: Engineered
Fragment
PDB:  
Structural/functional type: Function arises via a disorder to order transition
Functional classes: Molecular assembly
Functional subclasses: Protein-protein binding
Detection methods:
  1. X-ray crystallography

References:
  1. Margittai M, Fasshauer D, Jahn R, Langen R. "The Habc domain and the SNARE core complex are connected by a highly flexible linker." Biochemistry. 2003; 42(14): 4009-4014. PubMed: 12680753

Comments:
 



References

  1. Fasshauer D, Eliason WK, BrĂ¼nger AT, Jahn R. "Identification of a minimal core of the synaptic SNARE complex sufficient for reversible assembly and disassembly." Biochemistry. 1998; 37(29): 10354-62. PubMed: 9671503



Comments


The sequence was cited in Fasshauer (1998) and confirmed to match Swiss-Prot ST1A_RAT (P32851) for residues 1-206 which are characterized in this entry.


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