| General information | | DisProt: | DP00155 | | Name: | Syntaxin-1A | | Synonym(s): | STX1A_RAT
Synaptotagmin-associated 35 kDa protein
P35A
Neuron-specific antigen HPC-1
| | First appeared in release: | Release 2.0 (02/14/2005) | | UniProt: | P32851 | | UniGene: | Rn.9943 | | SwissProt: | STX1A_RAT | | TrEMBL: | | | NCBI (GI): | 417842 | | Source organism: | Rattus norvegicus (Rat) | | Sequence length: | 288 | | Percent disordered: | 12% | | Homologues: | |
| Native sequence |
10 20 30 40 50 60
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MKDRTQELRT AKDSDDDDDV TVTVDRDRFM DEFFEQVEEI RGFIDKIAEN VEEVKRKHSA - 60
ILASPNPDEK TKEELEELMS DIKKTANKVR SKLKSIEQSI EQEEGLNRSS ADLRIRKTQH - 120
STLSRKFVEV MSEYNATQSD YRERCKGRIQ RQLEITGRTT TSEELEDMLE SGNPAIFASG - 180
IIMDSSISKQ ALSEIETRHS EIIKLENSIR ELHDMFMDMA MLVESQGEMI DRIEYNVEHA - 240
VDYVERAVSD TKKAVKYQSK ARRKKIMIII CCVILGIIIA STIGGIFG
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| Functional narrative |
Syntaxin 1A is involved in membrane fusion and exocytosis. This membrane-bound member of the SNARE family interacts directly with SNAP25 and synaptobrevin (VAMP) to form the SNARE complex. This complex can then interact with ATPase NSF and indirectly with SNAPs to separate into its individual pieces. Syntaxin 1A can also bind with munc18, munc13, and their homologs to form stable complexes which regulate the formation of the SNARE complex. In the presence of munc18, the disordered region binds to munc18 causing the disordered region to alter its conformation. This change assists in the regulation of the SNARE complex. If mutations occur at L165A and E166A, in the linker region, munc18 will not bind to Syntaxin 1A (Margittai 2003).
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| Map of ordered and disordered regions |
Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.
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| Region 1 | | Type: | Disordered - Extended | | Name: | | | Location: | 149 - 182 | | Length: | 34 | | Region sequence: |
IQRQLEITGRTTTSEELEDMLESGNPAIFASGII | | Modification type: | Engineered
Fragment
| | PDB: | | | Structural/functional type: | Function arises via a disorder to order transition
| | Functional classes: | Molecular assembly
| | Functional subclasses: | Protein-protein binding
| Detection methods:
- X-ray crystallography
| References:
- Margittai M, Fasshauer D, Jahn R, Langen R. "The Habc domain and the SNARE core complex are connected by a highly flexible linker." Biochemistry. 2003; 42(14): 4009-4014. PubMed: 12680753
| Comments:
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| References |
- Fasshauer D, Eliason WK, Brünger AT, Jahn R. "Identification of a minimal core of the synaptic SNARE complex sufficient for reversible assembly and disassembly." Biochemistry. 1998; 37(29): 10354-62. PubMed: 9671503
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| Comments |
The sequence was cited in Fasshauer (1998) and confirmed to match Swiss-Prot ST1A_RAT (P32851) for residues 1-206 which are characterized in this entry.
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