Annotation for this protein is in progress - please check future releases for more complete information


DP00165: Pyridoxine/pyridoxamine 5'-phosphate oxidaseFASTA viewXML view

General information
DisProt:DP00165
Name:Pyridoxine/pyridoxamine 5'-phosphate oxidase
Synonym(s):PDXH_ECOLI
EC 1.4.3.5
PNP/PMP oxidase
PNPOx
Pyridoxal 5'-phosphate synthase
First appeared in release:Release 3.0 (02/17/2006)
UniProt:P0AFI7
UniGene: 
SwissProt: PDXH_ECOLI
TrEMBL:  
NCBI (GI): 84028916
Source organism:Escherichia coli (strain K12)
Sequence length:218
Percent disordered:29%
Homologues: 


Native sequence

        10         20         30         40         50         60
         |          |          |          |          |          |
MSDNDELQQI AHLRREYTKG GLRRRDLPAD PLTLFERWLS QACEAKLADP TAMVVATVDE - 60
HGQPYQRIVL LKHYDEKGMV FYTNLGSRKA HQIENNPRVS LLFPWHTLER QVMVIGKAER - 120
LSTLEVMKYF HSRPRDSQIG AWVSKQSSRI SARGILESKF LELKQKFQQG EVPLPSFWGG - 180
FRVSLEQIEF WQGGEHRLHD RFLYQRENDA WKIDRLAP

Region 1: 1-10 Region 2: 1-16 Region 4: 128-175 Region 3: 130-175

Map of ordered and disordered regions







Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.


Region 1
Type:Disordered
Name: 
Location:1 - 10
Length:10
Region sequence:

MSDNDELQQI

Modification type: Complex
Native
PDB:  
Structural/functional type: Function arises via a disorder to order transition
Functional classes: Modification site
Functional subclasses: Protein-protein binding
Detection methods:
  1. X-ray crystallography (100 K; K2PO4 (pH 7.5) 100 mM; protein 5 mg/ml; 2-mercaptoethanol 5 mM; HEPES (pH 6.8) 60 mM; KH2PO4/NH4H2PO4 0.65 M)
References:
  1. Safo MK, Musayev FN, Schirch V. "Structure of Escherichia coli pyridoxine 5'-phosphate oxidase in a tetragonal crystal form: insights into the mechanistic pathway of the enzyme." Acta Crystallogr D Biol Crystallogr. 2005; 61(Pt 5): 599-604. PubMed: 15858270
Comments:
Disorder in this region is found in monomer A of the tetragonal unit cell.


Region 2
Type:Disordered
Name: 
Location:1 - 16
Length:16
Region sequence:

MSDNDELQQIAHLRRE

Modification type: Complex
Native
PDB:  
Structural/functional type: Function arises via a disorder to order transition
Functional classes: Modification site
Functional subclasses: Protein-protein binding
Detection methods:
  1. X-ray crystallography (100 K; 2-mercaptoethanol 5 mM; HEPES (pH 6.8) 60 mM; K2PO4 (pH 7.5) 100 mM; KH2PO4/NH4H2PO4 0.65 M; protein 5 mg/ml)
References:
  1. Safo MK, Musayev FN, Schirch V. "Structure of Escherichia coli pyridoxine 5'-phosphate oxidase in a tetragonal crystal form: insights into the mechanistic pathway of the enzyme." Acta Crystallogr D Biol Crystallogr. 2005; 61(Pt 5): 599-604. PubMed: 15858270
Comments:
Disorder in this region is found in monomer B of the tetragonal unit cell.


Region 3
Type:Disordered
Name:domain 2
Location:130 - 175
Length:46
Region sequence:

FHSRPRDSQIGAWVSKQSSRISARGILESKFLELKQKFQQGEVPLP

Modification type: Complex
Native
PDB:  
Structural/functional type: Function arises from the disordered state
Functional classes: Modification site
Functional subclasses:  
Detection methods:
  1. X-ray crystallography (100 K; 2-mercaptoethanol 5 mM; HEPES (pH 6.8) 60 mM; K2PO4 (pH 7.5) 100 mM; KH2PO4/NH4H2PO4 0.65 M; protein 5 mg/ml)
References:
  1. Safo MK, Musayev FN, Schirch V. "Structure of Escherichia coli pyridoxine 5'-phosphate oxidase in a tetragonal crystal form: insights into the mechanistic pathway of the enzyme." Acta Crystallogr D Biol Crystallogr. 2005; 61(Pt 5): 599-604. PubMed: 15858270
Comments:
Disorder in this region is found in monomer A of the tetragonal unit cell.




Region 4
Type:Disordered
Name:domain 2
Location:128 - 175
Length:48
Region sequence:

KYFHSRPRDSQIGAWVSKQSSRISARGILESKFLELKQKFQQGEVPLP

Modification type: Complex
Native
PDB:  
Structural/functional type: Function arises from the disordered state
Functional classes: Modification site
Functional subclasses:  
Detection methods:
  1. X-ray crystallography (100 K; 2-mercaptoethanol 5 mM; HEPES (pH 6.8) 60 mM; K2PO4 (pH 7.5) 100 mM; KH2PO4/NH4H2PO4 0.65 M; protein 5 mg/ml)
References:
  1. Safo MK, Musayev FN, Schirch V. "Structure of Escherichia coli pyridoxine 5'-phosphate oxidase in a tetragonal crystal form: insights into the mechanistic pathway of the enzyme." Acta Crystallogr D Biol Crystallogr. 2005; 61(Pt 5): 599-604. PubMed: 15858270
Comments:
Disorder in this region is found in monomer B of the tetragonal unit cell.


References

  1. Safo MK, Mathews I, Musayev FN, di Salvo ML, Thiel DJ, Abraham DJ, Schirch V. "X-ray structure of Escherichia coli pyridoxine 5'-phosphate oxidase complexed with FMN at 1.8 A resolution." Structure Fold Des. 2000; 8(7): 751-62. PubMed: 10903950



Comments


The sequence referenced in the paper has an M at position 1. However, the SwissProt sequence does not include this M.


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