DP00175: Transcription factor 7-like 2FASTA viewXML view

General information
DisProt:DP00175
Name:Transcription factor 7-like 2
Synonym(s):TF7L2_HUMAN
HMG box transcription factor 4
T-cell-specific transcription factor 4
TCF-4
hTCF-4
T7L2
First appeared in release:Release 2.0 (02/14/2005)
UniProt:Q9NQB0
UniGene:Hs.593995
SwissProt: TF7L2_HUMAN
TrEMBL:  
NCBI (GI): 29337146
Source organism:Homo sapiens (Human)
Sequence length:619
Percent disordered:9%
Homologues: 


Native sequence

        10         20         30         40         50         60
         |          |          |          |          |          |
MPQLNGGGGD DLGANDELIS FKDEGEQEEK SSENSSAERD LADVKSSLVN ESETNQNSSS - 60
DSEAERRPPP RSESFRDKSR ESLEEAAKRQ DGGLFKGPPY PGYPFIMIPD LTSPYLPNGS - 120
LSPTARTLHF QSGSTHYSAY KTIEHQIAVQ YLQMKWPLLD VQAGSLQSRQ ALKDARSPSP - 180
AHIVSNKVPV VQHPHHVHPL TPLITYSNEH FTPGNPPPHL PADVDPKTGI PRPPHPPDIS - 240
PYYPLSPGTV GQIPHPLGWL VPQQGQPVYP ITTGGFRHPY PTALTVNASM SRFPPHMVPP - 300
HHTLHTTGIP HPAIVTPTVK QESSQSDVGS LHSSKHQDSK KEEEKKKPHI KKPLNAFMLY - 360
MKEMRAKVVA ECTLKESAAI NQILGRRWHA LSREEQAKYY ELARKERQLH MQLYPGWSAR - 420
DNYGKKKKRK RDKQPGETNE HSECFLNPCL SLPPITDLSA PKKCRARFGL DQQNNWCGPC - 480
RRKKKCVRYI QGEGSCLSPP SSDGSLLDSP PPSPNLLGSP PRDAKSQTEQ TQPLSLSLKP - 540
DPLAHLSMMP PPPALLLAEA THKASALCPN GALDLPPAAL QPAAPSSSIA QPSTSSLHSH - 600
SSLAGTQPQP LSLVTKSLE



Functional narrative    

TCF-4 is an HMG protein and a member of the TCF/LEF-family. Upon binding to beta-catenin, the newly formed complex moves from the cytoplasm to the nucleus where it plays a key role in the Wnt-signaling pathway and transcriptional activation of target genes. TCF-4 is involved in embryonal development and growth of normal and malignant colon epithilium. Mutations can elevate levels of TCF-4-catenin in colonic epithelia initiating the pathogenisis of gastrointestinal tumors. Highly acidic residues 1-56 are disordered in solution with ~96% random coil structure. Upon binding to beta-catenin the protein undergoes a disorder to order transition in which portions of the catenin-binding domain become structured.

Region 1: 1-56

Map of ordered and disordered regions







Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.


Region 1
Type:Disordered
Name:catenin-binding domain
Location:1 - 56
Length:56
Region sequence:

MPQLNGGGGDDLGANDELISFKDEGEQEEKSSENSSAERDLADVKSSLVNESETNQ

Modification type: Native
PDB: 1JPW:D
Structural/functional type: Function arises via a disorder to order transition
Functional classes: Molecular recognition effectors
Molecular assembly
Functional subclasses: Transactivation (transcriptional activation)
Protein-protein binding
Detection methods:
  1. Circular dichroism (CD) spectroscopy, far-UV
References:
  1. Knapp S, Zamai M, Volpi D, Nardese V, Avanzi N, Breton J, Plyte S, Flocco M, Marconi M, Isacchi A, Caiolfa VR. "Thermodynamics of the high-affinity interaction of TCF4 with beta-catenin." J Mol Biol. 2001; 306(5): 1179-1189. PubMed: 11237626

  2. Poy F, Lepourcelet M, Shivdasani RA, Eck MJ. "Structure of a human Tcf4-beta-catenin complex." Nat Struct Biol. 2001; 8(12): 1053-1057. PubMed: 11713476
Comments:
In the PDB file, the disorder to order transition upon binding with beta-catenin can be seen: residues 13-25 and 40-50 form alpha helicies upon binding to the armadillo repeats of catenin.

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