| General information | | DisProt: | DP00194 | | Name: | Stringent starvation protein B | | Synonym(s): | SSPB_ECOLI
SspB
| | First appeared in release: | Release 3.0 (02/17/2006) | | UniProt: | P0AFZ3 | | UniGene: | | | SwissProt: | SSPB_ECOLI | | TrEMBL: | | | NCBI (GI): | 84028797 | | Source organism: | Escherichia coli | | Sequence length: | 165 | | Percent disordered: | 33% | | Homologues: | |
| Native sequence |
10 20 30 40 50 60
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MDLSQLTPRR PYLLRAFYEW LLDNQLTPHL VVDVTLPGVQ VPMEYARDGQ IVLNIAPRAV - 60
GNLELANDEV RFNARFGGIP RQVSVPLAAV LAIYARENGA GTMFEPEAAY DEDTSIMNDE - 120
EASADNETVM SVIDGDKPDH DDDTHPDDEP PQPPRGGRPA LRVVK
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| Functional narrative |
The SspB adaptor binds peptide sequences in the stress-response regulator RseA and in ssrA-tagged proteins and delivers these molecules to the ATP-dependent bacterial protease AAA+ ClpXP for degradation. Seems to act in concert with sspA in the regulation of several proteins during exponential and stationary-phase growth. The exact function of sspB is not yet known.
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| Map of ordered and disordered regions |
Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.
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| Region 1 | | Type: | Disordered | | Name: | | | Location: | 111 - 165 | | Length: | 55 | | Region sequence: |
DEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPALRVVK | | Modification type: | Native
| | PDB: | | | Structural/functional type: | Function arises from the disordered state
| | Functional classes: | Molecular assembly
Entropic chain
| | Functional subclasses: | Flexible linkers/spacers
Substrate/ligand binding
Protein-protein binding
| Detection methods:
- Circular dichroism (CD) spectroscopy, far-UV (298 K; pH: 7.5; 1 mm pathlenght; 20 microM SspB in 10 mM potassium phosphate)
| References:
- Wah DA, Levchenko I, Rieckhof GE, Bolon DN, Baker TA, Sauer RT. "Flexible linkers leash the substrate binding domain of SspB to a peptide module that stabilizes delivery complexes with the AAA+ ClpXP protease." Mol Cell. 2003; 12(2): 355-63. PubMed: 14536075
| Comments:
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| References |
- Hersch GL, Baker TA, Sauer RT. "SspB delivery of substrates for ClpXP proteolysis probed by the design of improved degradation tags." Proc Natl Acad Sci U S A. 2004; 101(33): 12136-41. PubMed: 15297609
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