| General information | | DisProt: | DP00196 | | Name: | Calpastatin | | Synonym(s): | ICAL_HUMAN
Calpain inhibitor
Sperm BS-17 component
| | First appeared in release: | Release 3.0 (02/17/2006) | | UniProt: | P20810 | | UniGene: | Hs.436186 | | SwissProt: | ICAL_HUMAN | | TrEMBL: | | | NCBI (GI): | 126302556 | | Source organism: | Homo sapiens (Human) | | Sequence length: | 708 | | Percent disordered: | 20% | | Homologues: | |
| Native sequence |
10 20 30 40 50 60
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MNPTETKAIP VSQQMEGPHL PNKKKHKKQA VKTEPEKKSQ STKLSVVHEK KSQEGKPKEH - 60
TEPKSLPKQA SDTGSNDAHN KKAVSRSAEQ QPSEKSTEPK TKPQDMISAG GESVAGITAI - 120
SGKPGDKKKE KKSLTPAVPV ESKPDKPSGK SGMDAALDDL IDTLGGPEET EEENTTYTGP - 180
EVSDPMSSTY IEELGKREVT IPPKYRELLA KKEGITGPPA DSSKPIGPDD AIDALSSDFT - 240
CGSPTAAGKK TEKEESTEVL KAQSAGTVRS AAPPQEKKRK VEKDTMSDQA LEALSASLGT - 300
RQAEPELDLR SIKEVDEAKA KEEKLEKCGE DDETIPSEYR LKPATDKDGK PLLPEPEEKP - 360
KPRSESELID ELSEDFDRSE CKEKPSKPTE KTEESKAAAP APVSEAVSRT SMCSIQSAPP - 420
EPATLKGTVP DDAVEALADS LGKKEADPED GKPVMDKVKE KAKEEDREKL GEKEETIPPD - 480
YRLEEVKDKD GKPLLPKESK EQLPPMSEDF LLDALSEDFS GPQNASSLKF EDAKLAAAIS - 540
EVVSQTPAST TQAGAPPRDT SQSDKDLDDA LDKLSDSLGQ RQPDPDENKP MGDKVKEKAK - 600
AEHRDKLGER DDTIPPEYRH LLDDNGQDKP VKPPTKKSED SKKPADDQDP IDALSGDLDS - 660
CPSTTETSQN TAKDKCKKAA SSSKAPKNGG KAKDSAKTTE ETSKPKDD
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| Functional narrative |
Specific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmortem tenderization of meat and has been proposed to be involved in muscle protein degradation in living tissue. Contains four inhibitory domains.
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| Map of ordered and disordered regions |
Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.
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| Region 1 | | Type: | Disordered | | Name: | | | Location: | 137 - 277 | | Length: | 141 | | Region sequence: |
AVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGK
REVTIPPKYRELLAKKEGITGPPADSSKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEES
TEVLKAQSAGTVRSAAPPQEK | | Modification type: | Fragment
| | PDB: | | | Structural/functional type: | | | Functional classes: | | | Functional subclasses: | | Detection methods:
- Circular dichroism (CD) spectroscopy, far-UV (298 K; Na2HPO4 (pH 7.5) 10 mM; NaCl 150 mM)
- Sensitivity to proteolysis (298 K; EDTA 1 mM; mercaptoethanol 5 mM; NaCl 150 mM; Tris (pH 7.5) 20 mM)
- Nuclear magnetic resonance (NMR) (EDTA 1 mM; mercaptoethanol 5 mM; NaCl 150 mM; protein 50 mg/ml; Tris (pH 7.5) 20 mM)
| References:
- Csizmok V, Bokor M, Banki P, Klement E, Medzihradszky KF, Friedrich P, Tompa K, Tompa P. "Primary contact sites in intrinsically unstructured proteins: the case of calpastatin and microtubule-associated protein 2." Biochemistry. 2005; 44(10): 3955-64. PubMed: 15751971
| Comments:
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| Comments |
Toke reference provided by Maya. Moved from DP00686 due to duplication.
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| If you have any comments or wish to provide additional references to this protein or its disordered region(s), please click here to e-mail us. |
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