| General information | | DisProt: | DP00213 | | Name: | Histone-binding protein N1/N2 | | Synonym(s): | HIBN_XENLA
| | First appeared in release: | Release 2.0 (02/14/2005) | | UniProt: | P06180 | | UniGene: | Xl.48795 | | SwissProt: | HIBN_XENLA | | TrEMBL: | | | NCBI (GI): | 123127 | | Source organism: | Xenopus laevis (African clawed frog) | | Sequence length: | 590 | | Percent disordered: | 4% | | Homologues: | |
| Native sequence |
10 20 30 40 50 60
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MAEETAALST EKTEDTSTAP STSAEKADGI DIDTEAKRLM GAGQKHLVMK DVRSAVNLFQ - 60
EASSLLAKQY GETADECAEA FYSYGMSLLE LARLENGVLG NALEGMPEDD EEEAEKEEDP - 120
NIPSADNLDE KEREQLREQV YDAMAEDQRA PDDTSESEAK GKPEGDSKDK EADEKMKNGQ - 180
KETEKVTDDL KIDSASRDVP MDKSGKGEPP ESKDAETLVE QKESKPETLK EKSIETKEKD - 240
LSKEKTDAKE TANQSPDSTE VAEEKMDSEA SESKESTSIP PTENEANKPD DPEKMEEEEE - 300
GEDSEENEDG TEENEGTEEK ETEEEDVGNL QLAWEMLDLC KTIFKRQQSK EAQLKAAQAH - 360
QKLGEVCIES ENYSQAVEDF LACLNIQKEH LEEHDRLLAE THYHLGLAYQ YSSKHEEAIS - 420
HFTQSIGVIE KRMDVLTKQL EASVGELVDE VKKEMDELKD LLPDIKEKIE DSKEAQKNAT - 480
VTEKALKETL VGGSSGFSKE NGSTSSSSAV EKSGDSTVPV TNCVSDISHL VRKKRKTEEE - 540
SPLKDKDAKK SKQEPVANGA GNGDAVVPTN EEAEKAEEAS METATVESTA
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| Functional narrative |
This protein is involved in nucleosome assembly. It is bound to H3 and H4 in the absence of DNA, but released from H3 and H4 in the presence of DNA. (UniProt).
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| Map of ordered and disordered regions |
Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.
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| Region 1 | | Type: | Disordered | | Name: | nuclear location sequence (NLS) | | Location: | 532 - 552 | | Length: | 21 | | Region sequence: |
RKKRKTEEESPLKDKAKKSKG | | Modification type: | Complex
Engineered
Fragment
| | PDB: | 1PJN:A | | Structural/functional type: | Function arises from the disordered state
| | Functional classes: | Chaperones
| | Functional subclasses: | Nuclear localization
Flexible linkers/spacers
| Detection methods:
- X-ray crystallography (293 K; pH: 6; DTT; Sodium citrate)
| References:
- Fontes MR, Teh T, Jans D, Brinkworth RI, Kobe B. "Structural basis for the specificity of bipartite nuclear localization sequence binding by importin-alpha." J. Biol. Chem.. 2003; 278(30): 27981-7. PubMed: 12695505
| Comments:The numbering used by Fontes et al (2003) for the NLS--aa 335-355--differs slightly from UniProt--aa 532-552--for the same sequence. UniProt numbering is used in DP00213. Additionally, the authors used a synthetically constructed peptide which varies from UniProt (listed first) at these positions: D547A, A548K, K550S, S551K, K552G.
The Fontes et al (2003) experiment was performed in complex with importin-alpha.
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| References |
- Fontes MR, Teh T, Jans D, Brinkworth RI, Kobe B. "Structural basis for the specificity of bipartite nuclear localization sequence binding by importin-alpha." J. Biol. Chem.. 2003; 278(30): 27981-7. PubMed: 12695505
- Kleinschmidt JA, Dingwall C, Maier G, Franke WW. "Molecular characterization of a karyophilic, histone-binding protein: cDNA cloning, amino acid sequence and expression of nuclear protein N1/N2 of Xenopus laevis." Embo J. 1986; 5(13): 3547-52. PubMed: 3549279
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| Comments |
According to Kleinschmidt et al (1986), this protein shows aberrant migration on SDS-PAGE gels and is heat stable. These two properties strongly suggest that the protein is totally disordered but are not considered definitive evidence that it is, in fact, disordered.
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