| General information | | DisProt: | DP00220 | | Name: | Peripherin-2 | | Synonym(s): | PRPH2_BOVIN
Retinal degeneration slow protein
| | First appeared in release: | Release 3.0 (02/17/2006) | | UniProt: | P17810 | | UniGene: | Bt.12752 | | SwissProt: | PRPH2_BOVIN | | TrEMBL: | | | NCBI (GI): | 132211 | | Source organism: | Bos taurus (Bovine) | | Sequence length: | 345 | | Percent disordered: | 18% | | Homologues: | |
| Native sequence |
10 20 30 40 50 60
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ALLKVKFDQK KRVKLAQGLW LMNWFSVLAG IIIFGLGLFL KIELRKRSDV MNNSESHFVP - 60
NSLIGVGVLS CVFNSLAGKI CYDALDPAKY AKWKPWLKPY LAVCVLFNVV LFLVALCCFL - 120
LRGSLESTLA HGLKNGMKFY RDTDTPGRCF MKKTIDMLQI EFKCCGNNGF RDWFEIQWIS - 180
NRYLDFSSKE VKDRIKSNVD GRYLVDGVPF SCCNPNSPRP CIQYQLTNNS AHYSYDHQTE - 240
ELNLWLRGCR AALLSYYSNL MNTTGAVTLL VWLFEVTITV GLRYLHTALE GMANPEDPEC - 300
ESEGWLLEKS VPETWKAFLE SVKKLGKGNQ VEAEGEDAGQ APAAG
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| Functional narrative |
Peripherin/rds (P/rds) is a photoreceptor-specific membrane glycoprotein necessary for outer segment disc morphogenesis. P/rds, and it's non-glycosylated homologue rom-1, are members of the transmembrane 4 family of proteins, which together participate in photoreceptor disk morphogenesis and localize to rod and cone outer segment (OS) disk rims. Genetic defects affecting P/rds cause a variety of progressive retinal degenerations in humans and mice, and illustrate its importance for the formation and long-term stability of photoreceptor OSs. Although several hypotheses have been advanced for P/rds molecular function, a definitive role has not yet been assigned. In contrast to most tetraspanins, P/rds possesses a sizable (63 amino acid) C-terminus and the available molecular genetic evidence suggests that this region is important for protein function.
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| Map of ordered and disordered regions |
Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.
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| Region 1 | | Type: | Disordered - Extended | | Name: | C-terminal domain | | Location: | 283 - 345 | | Length: | 63 | | Region sequence: |
RYLHTALEGMANPEDPECESEGWLLEKSVPETWKAFLESVKKLGKGNQVEAEGEDAGQAP
AAG | | Modification type: | Fragment
Native
| | PDB: | | | Structural/functional type: | Function arises from the disordered state
| | Functional classes: | Unknown
| | Functional subclasses: | Unknown
| Detection methods:
- Sensitivity to proteolysis (310 K; pH: 7.5; 0.4 mg/ml protein; 2 microg/ml Proteinase K; 50 mM Tris-HCl, 150 mM NaCl)
- Fluorescence, intrinsic (298 K; pH: 7.5; 50 mM Tris-HCl, 150 mM NaCl, 1 mM TCEP; 5 microg/ml protein; excitation at 295nm, emission scan from 295 to 430nm)
- Circular dichroism (CD) spectroscopy, far-UV (283 K; pH: 7.5; 0.1 cm cell; 0.3 mg/ml protein; 50 mM Tris-HCl, 150 mM NaCl, 1 mM TCEP)
- Size exclusion/gel filtration chromatography (298 K; pH: 7.5; 0.2 ml/min flow rate; 0.5 to 1.5 mg protein; 1 cm x 46 cm Sephacryl S-100HR column; 50 mM Tris-HCl, 150 mM NaCl, 1 mM TCEP)
- Analytical ultracentrifugation (293 K; pH: 7.5; 1.0, 0.3 and 0.1 mg/ml protein; 500 microl aliquots; 50k rpm; 50 mM Tris-HCl, 150 mM NaCl, 1 mM TCEP)
| References:
- Ritter LM, Arakawa T, Goldberg AF. "Predicted and measured disorder in peripherin/rds, a retinal tetraspanin." Protein Pept Lett. 2005; 12(7): 677-86. PubMed: 16522184
| Comments:
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| References |
- Boesze-Battaglia K, Goldberg AF. "Photoreceptor renewal: a role for peripherin/rds." Int Rev Cytol. 2002; 217: 183-225. PubMed: 12019563
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