Annotation for this protein is in progress - please check future releases for more complete information


DP00229: Lupus La proteinFASTA viewXML view

General information
DisProt:DP00229
Name:Lupus La protein
Synonym(s):LA_HUMAN
Sjoegren syndrome type B antigen
SS-B
La ribonucleoprotein
La autoantigen
First appeared in release:Release 2.0 (02/14/2005)
UniProt:P05455
UniGene:Hs.632535
SwissProt: LA_HUMAN
TrEMBL:  
NCBI (GI): 125985
Source organism:Homo sapiens (Human)
Sequence length:408
Percent disordered:25%
Homologues: 


Native sequence

        10         20         30         40         50         60
         |          |          |          |          |          |
MAENGDNEKM AALEAKICHQ IEYYFGDFNL PRDKFLKEQI KLDEGWVPLE IMIKFNRLNR - 60
LTTDFNVIVE ALSKSKAELM EISEDKTKIR RSPSKPLPEV TDEYKNDVKN RSVYIKGFPT - 120
DATLDDIKEW LEDKGQVLNI QMRRTLHKAF KGSIFVVFDS IESAKKFVET PGQKYKETDL - 180
LILFKDDYFA KKNEERKQNK VEAKLRAKQE QEAKQKLEED AEMKSLEEKI GCLLKFSGDL - 240
DDQTCREDLH ILFSNHGEIK WIDFVRGAKE GIILFKEKAK EALGKAKDAN NGNLQLRNKE - 300
VTWEVLEGEV EKEALKKIIE DQQESLNKWK SKGRRFKGKG KGNKAAQPGS GKGKVQFQGK - 360
KTKFASDDEH DEHDENGATG PVKRAREETD KEEPASKQQK TENGAGDQ

Region 2: 1-5 Region 5: 1-8 Region 3: 190-194 Region 4: 225-230 Region 1: 326-408

Map of ordered and disordered regions







Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.


Region 1
Type:Disordered
Name: 
Location:326 - 408
Length:83
Region sequence:

LNKWKSKGRRFKGKGKGNKAAQPGSGKGKVQFQGKKTKFASDDEHDEHDENGATGPVKRA
REETDKEEPASKQQKTENGAGDQ

Modification type: Fragment
Native
PDB:  
Structural/functional type: Relationship to function unknown
Functional classes: Unknown
Functional subclasses: Unknown
Detection methods:
  1. Analytical ultracentrifugation (293 K; pH: 7; DTT 0.5 mM; KCl 100 mM; Tris-HCl (buffer) 20 mM)

  2. Nuclear magnetic resonance (NMR) (DTT (pH 7.0) 1 mM; KCl (buffer) 100 mM; Tris-HCl (buffer) 20 mM)

  3. Nuclear magnetic resonance (NMR) (DTT (pH 5.8 to 6.5) 1 mM; KCl (buffer) 100 mM; sodium acetate (buffer) 20 mM)

  4. Circular dichroism (CD) spectroscopy, far-UV (295 K; KF (pH 7) 100 mM; NaH2PO4 10 mM)
References:
  1. Jacks A, Babon J, Kelly G, Manolaridis I, Cary PD, Curry S, Conte MR. "Structure of the C-terminal domain of human La protein reveals a novel RNA recognition motif coupled to a helical nuclear retention element." Structure (Camb). 2003; 11(7): 833-43. PubMed: 12842046
Comments:
 



Region 2
Type:Disordered
Name: 
Location:1 - 5
Length:5
Region sequence:

MAENG

Modification type: Engineered
Fragment
PDB: 1YTY:A
Structural/functional type:  
Functional classes:  
Functional subclasses:  
Detection methods:
 
References:
  1. Teplova M, Yuan YR, Phan AT, Malinina L, Ilin S, Teplov A, Patel DJ. "Structural basis for recognition and sequestration of UUU(OH) 3' temini of nascent RNA polymerase III transcripts by La, a rheumatic disease autoantigen." Mol Cell. 2006; 21(1): 75-85. PubMed: 16387655
Comments:
This disordered protein entry is based solely on missing electron density in the Protein Data Bank.


Region 3
Type:Disordered
Name: 
Location:190 - 194
Length:5
Region sequence:

AKKNE

Modification type: Engineered
Fragment
PDB: 1YTY:A
Structural/functional type:  
Functional classes:  
Functional subclasses:  
Detection methods:
 
References:
  1. Teplova M, Yuan YR, Phan AT, Malinina L, Ilin S, Teplov A, Patel DJ. "Structural basis for recognition and sequestration of UUU(OH) 3' temini of nascent RNA polymerase III transcripts by La, a rheumatic disease autoantigen." Mol Cell. 2006; 21(1): 75-85. PubMed: 16387655
Comments:
This disordered protein entry is based solely on missing electron density in the Protein Data Bank.


Region 4
Type:Disordered
Name: 
Location:225 - 230
Length:6
Region sequence:

SLEEKI

Modification type: Fragment
PDB:  
Structural/functional type: Relationship to function unknown
Functional classes: Unknown
Functional subclasses: Unknown
Detection methods:
  1. Analytical ultracentrifugation (293 K; DTT (pH 7.0) 0.5 mM; KCl 100 mM; Tris-HCl 20 mM)

  2. Nuclear magnetic resonance (NMR) (pH: 7; DTT 1 mM; KCl 100 mM; Tris-HCl 20 mM)

  3. Nuclear magnetic resonance (NMR) (DTT (pH 5.8-6.5) 1 mM; KCl 100 mM; sodium acetate 20 mM)

  4. Circular dichroism (CD) spectroscopy, far-UV (295 K; KF (pH 7.0) 100 mM; NaH2PO4 10 mM)
References:
  1. Jacks A, Babon J, Kelly G, Manolaridis I, Cary PD, Curry S, Conte MR. "Structure of the C-terminal domain of human La protein reveals a novel RNA recognition motif coupled to a helical nuclear retention element." Structure (Camb). 2003; 11(7): 833-43. PubMed: 12842046
Comments:
 



Region 5
Type:Disordered
Name: 
Location:1 - 8
Length:8
Region sequence:

MAENGDNE

Modification type: Native
PDB:  
Structural/functional type: Function arises from the disordered state
Functional classes: Unknown
Functional subclasses: Unknown
Detection methods:
  1. Nuclear magnetic resonance (NMR) (293 K; pH: 7; DTT 1 mM; KCl 100 mM; Tris-HCl 20 mM)
References:
  1. Alfano C, Sanfelice D, Babon J, Kelly G, Jacks A, Curry S, Conte MR. "Structural analysis of cooperative RNA binding by the La motif and central RRM domain of human La protein." Nat Struct Mol Biol. 2004; 11(4): 323-9. PubMed: 15004549
Comments:
 



References

  1. Chambers JC, Kenan D, Martin BJ, Keene JD. "Genomic structure and amino acid sequence domains of the human La autoantigen." J Biol Chem. 1988; 263(34): 18043-51. PubMed: 3192525

  2. Chan EK, Sullivan KF, Tan EM. "Ribonucleoprotein SS-B/La belongs to a protein family with consensus sequences for RNA-binding." Nucleic Acids Res. 1989; 17(6): 2233-44. PubMed: 2468131


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