| Region 1 |
| Type: | Disordered |
| Name: | activation domain |
| Location: | 1 - 200 |
| Length: | 200 |
| Region sequence: |
MNNPSETSKPSMESGDGNTGTQTNGLDFQKQPVPVGGAISTAQAQAFLGHLHQVQLAGTS
LQAAAQSLNVQSKSNEESGDSQQPSQPSQQPSVQAAIPQTQLMLAGGQITGLTLTPAQQQ
LLLQQAQAQAQLLAAAVQQHSASQQHSAAGATISASAATPMTQIPLSQPIQIAQDLQQLQ
QLQQQNLNLQQFVLVHPTTN |
| Modification type: | Fragment
Monomeric
Native
|
| PDB: | |
| Structural/functional type: | Function arises from the disordered state
|
| Functional classes: | Molecular recognition effectors
|
| Functional subclasses: | Protein-protein binding
|
Detection methods:
- Small-angle X-ray scattering (SAXS)
- Nuclear magnetic resonance (NMR)
- Circular dichroism (CD) spectroscopy, far-UV
- Fluorescence, intrinsic
|
References:
- Lee L, Stollar E, Chang J, Grossmann JG, O'Brien R, Ladbury J, Carpenter B, Roberts S, Luisi B. "Expression of the Oct-1 transcription factor and characterization of its interactions with the Bob1 coactivator." Biochemistry. 2001; 40(22): 6580-8. PubMed: 11380252
|
Comments:
|
| Region 2 |
| Type: | Disordered |
| Name: | activation domain |
| Location: | 1 - 200 |
| Length: | 200 |
| Region sequence: |
MNNPSETSKPSMESGDGNTGTQTNGLDFQKQPVPVGGAISTAQAQAFLGHLHQVQLAGTS
LQAAAQSLNVQSKSNEESGDSQQPSQPSQQPSVQAAIPQTQLMLAGGQITGLTLTPAQQQ
LLLQQAQAQAQLLAAAVQQHSASQQHSAAGATISASAATPMTQIPLSQPIQIAQDLQQLQ
QLQQQNLNLQQFVLVHPTTN |
| Modification type: | Complex
Fragment
Native
|
| PDB: | |
| Structural/functional type: | Function arises from the disordered state
|
| Functional classes: | Molecular recognition effectors
|
| Functional subclasses: | Protein-protein binding
|
Detection methods:
- Circular dichroism (CD) spectroscopy, far-UV
|
References:
- Lee L, Stollar E, Chang J, Grossmann JG, O'Brien R, Ladbury J, Carpenter B, Roberts S, Luisi B. "Expression of the Oct-1 transcription factor and characterization of its interactions with the Bob1 coactivator." Biochemistry. 2001; 40(22): 6580-8. PubMed: 11380252
|
Comments:There was no increase in structure when a fragment of Oct-1 containing the activation domain was incubated with the binding partner, Bob-1.
|
| Region 3 |
| Type: | Disordered |
| Name: | |
| Location: | 355 - 378 |
| Length: | 24 |
| Region sequence: |
NLSSDSSLSSPSALNSPGIEGLSR |
| Modification type: | |
| PDB: | |
| Structural/functional type: | Function arises from the disordered state
|
| Functional classes: | Entropic chain
|
| Functional subclasses: | Flexible linkers/spacers
|
Detection methods:
- X-ray crystallography
|
References:
- Dekker N, Cox M, Boelens R, Verrijzer CP, van der Vliet PC, Kaptein R. "Solution structure of the POU-specific DNA-binding domain of Oct-1." Nature. 1993; 362(6423): 852-5. PubMed: 8479524
- Klemm JD, Rould MA, Aurora R, Herr W, Pabo CO. "Crystal structure of the Oct-1 POU domain bound to an octamer site: DNA recognition with tethered DNA-binding modules." Cell. 1994; 77(1): 21-32. PubMed: 8156594
|
Comments:
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