Annotation for this protein is in progress - please check future releases for more complete information


DP00278: Gap junction alpha-1 proteinFASTA viewXML view

General information
DisProt:DP00278
Name:Gap junction alpha-1 protein
Synonym(s):CXA1_RAT
Connexin-43
Cx43
Gap junction 43 kDa heart protein
First appeared in release:Release 3.0 (02/17/2006)
UniProt:P08050
UniGene:Rn.10346
SwissProt: CXA1_RAT
TrEMBL:  
NCBI (GI): 117708
Source organism:Rattus norvegicus (Rat)
Sequence length:381
Percent disordered:29%
Homologues: 


Native sequence

        10         20         30         40         50         60
         |          |          |          |          |          |
GDWSALGKLL DKVQAYSTAG GKVWLSVLFI FRILLLGTAV ESAWGDEQSA FRCNTQQPGC - 60
ENVCYDKSFP ISHVRFWVLQ IIFVSVPTLL YLAHVFYVMR KEEKLNKKEE ELKVAQTDGV - 120
NVEMHLKQIE IKKFKYGIEE HGKVKMRGGL LRTYIISILF KSVFEVAFLL IQWYIYGFSL - 180
SAVYTCKRDP CPHQVDCFLS RPTEKTIFII FMLVVSLVSL ALNIIELFYV FFKGVKDRVK - 240
GRSDPYHATT GPLSPSKDCG SPKYAYFNGC SSPTAPLSPM SPPGYKLVTG DRNNSSCRNY - 300
NKQASEQNWA NYSAEQNRMG QAGSTISNSH AQPFDFPDDN QNAKKVAAGH ELQPLAIVDQ - 360
RPSSRASSRA SSRPRPDDLE I

Region 1: 250-313 Region 2: 326-338 Region 3: 348-381

Map of ordered and disordered regions







Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.


Region 1
Type:Disordered
Name: 
Location:250 - 313
Length:64
Region sequence:

TGPLSPSKDCGSPKYAYFNGCSSPTAPLSPMSPPGYKLVTGDRNNSSCRNYNKQASEQNW
ANYS

Modification type: Fragment
PDB:  
Structural/functional type: Function arises via a disorder to order transition
Functional classes: Entropic chain
Molecular assembly
Functional subclasses: Flexible linkers/spacers
Phosphorylation
Protein-protein binding
Detection methods:
  1. Nuclear magnetic resonance (NMR) (280 K; pH: 5.8; phosphate saline (buffer))
References:
  1. Duffy HS, Ashton AW, O'Donnell P, Coombs W, Taffet SM, Delmar M, Spray DC. "Regulation of connexin43 protein complexes by intracellular acidification." Circ Res. 2004; 94(2): 215-22. PubMed: 14699011

  2. Duffy HS, Sorgen PL, Girvin ME, O'Donnell P, Coombs W, Taffet SM, Delmar M, Spray DC. "pH-dependent intramolecular binding and structure involving Cx43 cytoplasmic domains." J Biol Chem. 2002; 277(39): 36706-14. PubMed: 12151412

  3. Seki A, Duffy HS, Coombs W, Spray DC, Taffet SM, Delmar M. "Modifications in the biophysical properties of connexin43 channels by a peptide of the cytoplasmic loop region." Circ Res. 2004; 95(4): e22-8. PubMed: 15284189

  4. Sorgen PL, Duffy HS, Sahoo P, Coombs W, Delmar M, Spray DC. "Structural changes in the carboxyl terminus of the gap junction protein connexin43 indicates signaling between binding domains for c-Src and zonula occludens-1." J Biol Chem. 2004; 279(52): 54695-701. PubMed: 15492000
Comments:
 



Region 2
Type:Disordered
Name: 
Location:326 - 338
Length:13
Region sequence:

ISNSHAQPFDFPD

Modification type: Fragment
PDB:  
Structural/functional type: Function arises via a disorder to order transition
Functional classes: Molecular assembly
Entropic chain
Functional subclasses: Flexible linkers/spacers
Phosphorylation
Protein-protein binding
Detection methods:
  1. Nuclear magnetic resonance (NMR) (280 K; pH: 5.8; phosphate saline (buffer))
References:
  1. Duffy HS, Ashton AW, O'Donnell P, Coombs W, Taffet SM, Delmar M, Spray DC. "Regulation of connexin43 protein complexes by intracellular acidification." Circ Res. 2004; 94(2): 215-22. PubMed: 14699011

  2. Duffy HS, Sorgen PL, Girvin ME, O'Donnell P, Coombs W, Taffet SM, Delmar M, Spray DC. "pH-dependent intramolecular binding and structure involving Cx43 cytoplasmic domains." J Biol Chem. 2002; 277(39): 36706-14. PubMed: 12151412

  3. Seki A, Duffy HS, Coombs W, Spray DC, Taffet SM, Delmar M. "Modifications in the biophysical properties of connexin43 channels by a peptide of the cytoplasmic loop region." Circ Res. 2004; 95(4): e22-8. PubMed: 15284189

  4. Sorgen PL, Duffy HS, Sahoo P, Coombs W, Delmar M, Spray DC. "Structural changes in the carboxyl terminus of the gap junction protein connexin43 indicates signaling between binding domains for c-Src and zonula occludens-1." J Biol Chem. 2004; 279(52): 54695-701. PubMed: 15492000
Comments:
 



Region 3
Type:Disordered
Name: 
Location:348 - 381
Length:34
Region sequence:

AGHELQPLAIVDQRPSSRASSRASSRPRPDDLEI

Modification type: Fragment
PDB:  
Structural/functional type: Function arises via a disorder to order transition
Functional classes: Molecular assembly
Entropic chain
Functional subclasses: Flexible linkers/spacers
Phosphorylation
Protein-protein binding
Detection methods:
  1. Nuclear magnetic resonance (NMR) (280 K; pH: 5.8; phosphate saline (buffer))
References:
  1. Duffy HS, Ashton AW, O'Donnell P, Coombs W, Taffet SM, Delmar M, Spray DC. "Regulation of connexin43 protein complexes by intracellular acidification." Circ Res. 2004; 94(2): 215-22. PubMed: 14699011

  2. Duffy HS, Sorgen PL, Girvin ME, O'Donnell P, Coombs W, Taffet SM, Delmar M, Spray DC. "pH-dependent intramolecular binding and structure involving Cx43 cytoplasmic domains." J Biol Chem. 2002; 277(39): 36706-14. PubMed: 12151412

  3. Seki A, Duffy HS, Coombs W, Spray DC, Taffet SM, Delmar M. "Modifications in the biophysical properties of connexin43 channels by a peptide of the cytoplasmic loop region." Circ Res. 2004; 95(4): e22-8. PubMed: 15284189

  4. Sorgen PL, Duffy HS, Sahoo P, Coombs W, Delmar M, Spray DC. "Structural changes in the carboxyl terminus of the gap junction protein connexin43 indicates signaling between binding domains for c-Src and zonula occludens-1." J Biol Chem. 2004; 279(52): 54695-701. PubMed: 15492000
Comments:
 



References

  1. Beyer EC, Paul DL, Goodenough DA. "Connexin43: a protein from rat heart homologous to a gap junction protein from liver." J Cell Biol. 1987; 105(6 Pt 1): 2621-9. PubMed: 2826492

  2. Granot I, Bechor E, Barash A, Dekel N. "Connexin43 in rat oocytes: developmental modulation of its phosphorylation." Biol Reprod. 2002; 66(3): 568-73. PubMed: 11870059

  3. Maza J, Das Sarma J, Koval M. "Defining a minimal motif required to prevent connexin oligomerization in the endoplasmic reticulum." J Biol Chem. 2005; 280(22): 21115-21. PubMed: 15817491

  4. Mitchell JA, Lye SJ. "Regulation of connexin43 expression by c-fos and c-jun in myometrial cells." Cell Commun Adhes. 2001; 8(4-6): 299-302. PubMed: 12064606


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