| General information | | DisProt: | DP00292 | | Name: | Phosphorelay protein luxU | | Synonym(s): | LUXU_VIBHA
| | First appeared in release: | Release 3.0 (02/17/2006) | | UniProt: | P0C5S4 | | UniGene: | | | SwissProt: | LUXU_VIBHA | | TrEMBL: | | | NCBI (GI): | 160409944 | | Source organism: | Vibrio harveyi | | Sequence length: | 114 | | Percent disordered: | 18% | | Homologues: | |
| Native sequence |
10 20 30 40 50 60
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MNTDVLNQQK IEELSAEIGS DNVPVLLDIF LGEMDSYIGT LTELQGSEQL LYLKEISHAL - 60
KSSAASFGAD RLCERAIAID KKAKANQLQE QGMETSEMLA LLHITRDAYR SWTN
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| Functional narrative |
Phosphorelay protein which receives sensory signals from luxN and luxP and transmits them to luxO, at low cell density. LuxN and luxP transfer a phosphoryl group to luxU on His-58 and this phosphoryl group is further transferred to luxO. At high cell density, as luxU could function to establish an equilibrium between the aspartyl-phosphate of luxN and the aspartyl-phosphate of luxO, luxU transfers phosphate from luxO to luxN (and probably luxP) and finally phosphate is drained from the system.
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| Map of ordered and disordered regions |
Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.
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| Region 1 | | Type: | Disordered | | Name: | | | Location: | 17 - 25 | | Length: | 9 | | Region sequence: |
EIGSDNVPV | | Modification type: | Engineered
| | PDB: | | | Structural/functional type: | | | Functional classes: | | | Functional subclasses: | | Detection methods:
- Nuclear magnetic resonance (NMR) (294 K; pH: 6.4; 2H2O 10 %; leupeptin (protease inhibitor); NaCl 300 mM; pepstatin (protease inhibitor))
| References:
- Ulrich DL, Kojetin D, Bassler BL, Cavanagh J, Loria JP. "Solution structure and dynamics of LuxU from Vibrio harveyi, a phosphotransferase protein involved in bacterial quorum sensing." J Mol Biol. 2005; 347(2): 297-307. PubMed: 15740742
| Comments:
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| Region 2 | | Type: | Disordered | | Name: | | | Location: | 86 - 92 | | Length: | 7 | | Region sequence: |
NQLQEQG | | Modification type: | Engineered
| | PDB: | | | Structural/functional type: | | | Functional classes: | | | Functional subclasses: | | Detection methods:
- Nuclear magnetic resonance (NMR) (294 K; pH: 6.4; 2H2O 10 %; leupeptin (protease inhibitor); NaCl 300 mM; pepstatin (protease inhibitor))
| References:
- Ulrich DL, Kojetin D, Bassler BL, Cavanagh J, Loria JP. "Solution structure and dynamics of LuxU from Vibrio harveyi, a phosphotransferase protein involved in bacterial quorum sensing." J Mol Biol. 2005; 347(2): 297-307. PubMed: 15740742
| Comments:
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| Region 3 | | Type: | Disordered | | Name: | | | Location: | 45 - 46 | | Length: | 2 | | Region sequence: |
QG | | Modification type: | Engineered
| | PDB: | | | Structural/functional type: | | | Functional classes: | | | Functional subclasses: | | Detection methods:
- Nuclear magnetic resonance (NMR) (294 K; pH: 6.4; 2H2O 10 %; leupeptin (protease inhibitor); NaCl 300 mM; pepstatin (protease inhibitor))
| References:
- Ulrich DL, Kojetin D, Bassler BL, Cavanagh J, Loria JP. "Solution structure and dynamics of LuxU from Vibrio harveyi, a phosphotransferase protein involved in bacterial quorum sensing." J Mol Biol. 2005; 347(2): 297-307. PubMed: 15740742
| Comments:
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| Region 4 | | Type: | Disordered | | Name: | | | Location: | 112 - 114 | | Length: | 3 | | Region sequence: |
WTN | | Modification type: | Engineered
| | PDB: | | | Structural/functional type: | | | Functional classes: | | | Functional subclasses: | | Detection methods:
- Nuclear magnetic resonance (NMR) (294 K; pH: 6.4; 2H2O 10 %; leupeptin (protease inhibitor); NaCl 300 mM; pepstatin (protease inhibitor))
| References:
- Ulrich DL, Kojetin D, Bassler BL, Cavanagh J, Loria JP. "Solution structure and dynamics of LuxU from Vibrio harveyi, a phosphotransferase protein involved in bacterial quorum sensing." J Mol Biol. 2005; 347(2): 297-307. PubMed: 15740742
| Comments:
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