| General information | | DisProt: | DP00293 | | Name: | Prokaryotic ubiquitin-like protein pup | | Synonym(s): | PUP_MYCTU
Bacterial ubiquitin-like modifier
| | First appeared in release: | Release 5.5 (11/17/2010) | | UniProt: | O33246 | | UniGene: | | | SwissProt: | PUP_MYCTU | | TrEMBL: | | | NCBI (GI): | 81669083 | | Source organism: | Mycobacterium tuberculosis | | Sequence length: | 64 | | Percent disordered: | 77% | | Homologues: | |
| Native sequence |
10 20 30 40 50 60
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MAQEQTKRGG GGGDDDDIAG STAAGQERRE KLTEETDDLL DEIDDVLEEN AEDFVRAYVQ - 60
KGGQ
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| Functional narrative |
Protein modifier that is covalently attached to lysine residues of substrate proteins, thereby targeting them for proteasomal degradation. The tagging system is termed pupylation. Identified substrates are the fabD, panB and mpa proteins.
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| Map of ordered and disordered regions |
Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.
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| Region 1 | | Type: | Disordered | | Name: | | | Location: | 1 - 31 | | Length: | 31 | | Region sequence: |
MAQEQTKRGGGGGDDDDIAGSTAAGQERREK | | Modification type: | Native
| | PDB: | | | Structural/functional type: | Function arises from the disordered state
| | Functional classes: | | | Functional subclasses: | | Detection methods:
- Nuclear magnetic resonance (NMR) (293 K; pH: 6.8; )
| References:
- Chen X, Solomon WC, Kang Y, Cerda-Maira F, Darwin KH, Walters KJ. "Prokaryotic ubiquitin-like protein pup is intrinsically disordered." J Mol Biol. 2009; 392(1): 208-17. PubMed: 19607839
- Liao S, Shang Q, Zhang X, Zhang J, Xu C, Tu X.
Biochem J. 2009 Jul 7. "PUP, a prokaryotic ubiquitin-like protein, is an an intrinsically disordered protein." Biochem J. 2009. PubMed: 18832610
| Comments:
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| Region 2 | | Type: | Disordered | | Name: | | | Location: | 33 - 38 | | Length: | 6 | | Region sequence: |
TEETDD | | Modification type: | Native
| | PDB: | | | Structural/functional type: | Function arises via an order to disorder transition
| | Functional classes: | | | Functional subclasses: | Protein-protein binding
| Detection methods:
- Circular dichroism (CD) spectroscopy, far-UV (293 K; pH: 6.5; )
| References:
There are no documents referencing this region. | Comments:
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| Region 3 | | Type: | Disordered | | Name: | | | Location: | 41 - 45 | | Length: | 5 | | Region sequence: |
DEIDD | | Modification type: | Complex
| | PDB: | | | Structural/functional type: | Function arises from the disordered state
| | Functional classes: | Modification site
Molecular recognition effectors
| | Functional subclasses: | Protein-protein binding
| Detection methods:
- Circular dichroism (CD) spectroscopy, far-UV
- Nuclear magnetic resonance (NMR)
| References:
There are no documents referencing this region. | Comments:
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| Region 4 | | Type: | Disordered | | Name: | | | Location: | 50 - 51 | | Length: | 2 | | Region sequence: |
NA | | Modification type: | Native
| | PDB: | | | Structural/functional type: | Function arises via an order to disorder transition
| | Functional classes: | | | Functional subclasses: | Protein-protein binding
| Detection methods:
- Nuclear magnetic resonance (NMR) (293 K; pH: 6.8; )
| References:
There are no documents referencing this region. | Comments:
|
| Region 5 | | Type: | Disordered - Extended | | Name: | | | Location: | 60 - 64 | | Length: | 5 | | Region sequence: |
QKGGQ | | Modification type: | Native
| | PDB: | | | Structural/functional type: | Function arises via an order to disorder transition
| | Functional classes: | | | Functional subclasses: | Protein-protein binding
| Detection methods:
- Nuclear magnetic resonance (NMR) (293 K; pH: 6.8; )
| References:
There are no documents referencing this region. | Comments:
|
| References |
- Liao S, Shang Q, Zhang X, Zhang J, Xu C, Tu X.
Biochem J. 2009 Jul 7. "PUP, a prokaryotic ubiquitin-like protein, is an an intrinsically disordered protein." Biochem J. 2009. PubMed: 18832610
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| Comments |
Author Verified 11-5-2010 PubMed: 18832610
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