Annotation for this protein is in progress - please check future releases for more complete information


DP00298: Bcl-2-like protein 1FASTA viewXML view

General information
DisProt:DP00298
Name:Bcl-2-like protein 1
Synonym(s):B2CL1_HUMAN
Bcl2-L-1
Apoptosis regulator Bcl-X
First appeared in release:Release 3.0 (02/17/2006)
UniProt:Q07817
UniGene:Hs.516966
SwissProt: B2CL1_HUMAN
TrEMBL:  
NCBI (GI): 728955
Source organism:Homo sapiens (Human)
Sequence length:233
Percent disordered:30%
Homologues: 


Native sequence

        10         20         30         40         50         60
         |          |          |          |          |          |
MSQSNRELVV DFLSYKLSQK GYSWSQFSDV EENRTEAPEG TESEMETPSA INGNPSWHLA - 60
DSPAVNGATG HSSSLDAREV IPMAAVKQAL REAGDEFELR YRRAFSDLTS QLHITPGTAY - 120
QSFEQVVNEL FRDGVNWGRI VAFFSFGGAL CVESVDKEMQ VLVSRIAAWM ATYLNDHLEP - 180
WIQENGGWDT FVELYGNNAA AESRKGQERF NRWFLTGMTV AGVVLLGSLF SRK

Region 5: 28-79 Region 1: 28-80 Region 3: 28-81 Region 2: 101-103 Region 4: 197-209 Region 6: 198-209

Map of ordered and disordered regions







Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.


Region 1
Type:Disordered
Name: 
Location:28 - 80
Length:53
Region sequence:

SDVEENRTEAPEGTESEMETPSAINGNPSWHLADSPAVNGATGHSSSLDAREV

Modification type: Engineered
Fragment
Monomeric
PDB: 1LXL:A, 1R2G:A
Structural/functional type:  
Functional classes:  
Functional subclasses:  
Detection methods:
  1. X-ray crystallography (protein (25 mg/ml); Tris (pH 7.4) 50 mM; PEG 4000 (28-31% (w/v)); ammonium acetate 0.2 M; sodium citrate (pH 7.5) 0.1 M)

  2. Nuclear magnetic resonance (NMR) (303 K; H2O/2H2O (9/1); perdeuterated DTT 5 mM; perdeuterated EDTA 1 mM; protein (1-3 nM); sodium phosphate (pH 7.3) 20 mM)

  3. Nuclear magnetic resonance (NMR) (303 K; 2H2O; perdeuterated DTT 5 mM; perdeuterated EDTA 1 mM; protein (1-3 nM); sodium phosphate (pH 7.3) 20 mM)
References:
  1. Muchmore SW, Sattler M, Liang H, Meadows RP, Harlan JE, Yoon HS, Nettesheim D, Chang BS, Thompson CB, Wong SL, Ng SL, Fesik SW. "X-ray and NMR structure of human Bcl-xL, an inhibitor of programmed cell death." Nature. 1996; 381(6580): 335-41. PubMed: 8692274
Comments:
The PDB file 1R2G has this region being disordered from S28 to I81.




Region 2
Type:Disordered
Name: 
Location:101 - 103
Length:3
Region sequence:

YRR

Modification type: Engineered
Fragment
Monomeric
PDB: 1R2D:A, 1MAZ:A, 1LXL:A
Structural/functional type: Relationship to function unknown
Functional classes: Unknown
Functional subclasses: Unknown
Detection methods:
  1. X-ray crystallography (ammonium acetate 0.2 M; PEG 4000 (28-31% (w/v)); protein (25 mg/ml); sodium citrate (pH 7.5) 0.1 M; Tris (pH 7.4) 50 mM)

  2. Nuclear magnetic resonance (NMR) (303 K; H2O/2H2O (9/1); perdeuterated DTT 5 mM; perdeuterated EDTA 1 mM; protein (1-3 nM); sodium phosphate (pH 7.3) 20 mM)

  3. Nuclear magnetic resonance (NMR) (303 K; 2H2O; perdeuterated DTT 5 mM; perdeuterated EDTA 1 mM; protein (1-3 nM); sodium phosphate (pH 7.3) 20 mM)
References:
  1. Muchmore SW, Sattler M, Liang H, Meadows RP, Harlan JE, Yoon HS, Nettesheim D, Chang BS, Thompson CB, Wong SL, Ng SL, Fesik SW. "X-ray and NMR structure of human Bcl-xL, an inhibitor of programmed cell death." Nature. 1996; 381(6580): 335-41. PubMed: 8692274
Comments:
 



Region 3
Type:Disordered
Name: 
Location:28 - 81
Length:54
Region sequence:

SDVEENRTEAPEGTESEMETPSAINGNPSWHLADSPAVNGATGHSSSLDAREVI

Modification type:  
PDB: 1MAZ:A, 1R2D:A, 1R2H:A, 1R2I:A
Structural/functional type:  
Functional classes:  
Functional subclasses:  
Detection methods:
 
References:
  1. Manion MK, O'Neill JW, Giedt CD, Kim KM, Zhang KY, Hockenbery DM. "Bcl-XL mutations suppress cellular sensitivity to antimycin A." J Biol Chem. 2004; 279(3): 2159-65. PubMed: 14534311
Comments:
 



Region 4
Type:Disordered
Name: 
Location:197 - 209
Length:13
Region sequence:

NNAAAESRKGQER

Modification type:  
PDB: 1R2D:A, 1R2G:A, 1R2H:A, 1RDE:A
Structural/functional type:  
Functional classes:  
Functional subclasses:  
Detection methods:
 
References:
  1. Manion MK, O'Neill JW, Giedt CD, Kim KM, Zhang KY, Hockenbery DM. "Bcl-XL mutations suppress cellular sensitivity to antimycin A." J Biol Chem. 2004; 279(3): 2159-65. PubMed: 14534311
Comments:
This disordered region contains an extra poly-histine tail at the C-terminus of the fragment that was added in the experiment to facilitate purification.


Region 5
Type:Disordered
Name: 
Location:28 - 79
Length:52
Region sequence:

SDVEENRTEAPEGTESEMETPSAINGNPSWHLADSPAVNGATGHSSSLDARE

Modification type:  
PDB: 1R2E:A
Structural/functional type:  
Functional classes:  
Functional subclasses:  
Detection methods:
 
References:
  1. Manion MK, O'Neill JW, Giedt CD, Kim KM, Zhang KY, Hockenbery DM. "Bcl-XL mutations suppress cellular sensitivity to antimycin A." J Biol Chem. 2004; 279(3): 2159-65. PubMed: 14534311
Comments:
 



Region 6
Type:Disordered
Name: 
Location:198 - 209
Length:12
Region sequence:

NAAAESRKGQER

Modification type: Engineered
Fragment
PDB: 1R2I:A
Structural/functional type:  
Functional classes:  
Functional subclasses:  
Detection methods:
 
References:
  1. Manion MK, O'Neill JW, Giedt CD, Kim KM, Zhang KY, Hockenbery DM. "Bcl-XL mutations suppress cellular sensitivity to antimycin A." J Biol Chem. 2004; 279(3): 2159-65. PubMed: 14534311
Comments:
This disordered region contains an extra poly-histine tail at the C-terminus of the fragment that was added in the experiment to facilitate purification.

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