| General information | | DisProt: | DP00326 | | Name: | DNA ligase | | Synonym(s): | DNLJ_ENTFA
EC=6.5.1.2
Polydeoxyribonucleotide synthase [NAD+]
| | First appeared in release: | Release 3.0 (02/17/2006) | | UniProt: | Q837V6 | | UniGene: | | | SwissProt: | DNLJ_ENTFA | | TrEMBL: | | | NCBI (GI): | 81437298 | | Source organism: | Enterococcus faecalis (Streptococcus faecalis) | | Sequence length: | 676 | | Percent disordered: | 1% | | Homologues: | |
| Native sequence |
10 20 30 40 50 60
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MEQQPLTLTA ATTRAQELRK QLNQYSHEYY VKDQPSVEDY VYDRLYKELV DIETEFPDLI - 60
TPDSPTQRVG GKVLSGFEKA PHDIPMYSLN DGFSKEDIFA FDERVRKAIG KPVAYCCELK - 120
IDGLAISLRY ENGVFVRGAT RGDGTVGENI TENLRTVRSV PMRLTEPISV EVRGECYMPK - 180
QSFVALNEER EENGQDIFAN PRNAAAGSLR QLDTKIVAKR NLNTFLYTVA DFGPMKAKTQ - 240
FEALEELSAI GFRTNPERQL CQSIDEVWAY IEEYHEKRST LPYEIDGIVI KVNEFALQDE - 300
LGFTVKAPRW AIAYKFPPEE AETVVEDIEW TIGRTGVVTP TAVMAPVRVA GTTVSRASLH - 360
NADFIQMKDI RLNDHVIIYK AGDIIPEVAQ VLVEKRAADS QPYEMPTHCP ICHSELVHLD - 420
EEVALRCINP KCPAQIKEGL NHFVSRNAMN IDGLGPRVLA QMYDKGLVKD VADLYFLTEE - 480
QLMTLDKIKE KSANNIYTAI QGSKENSVER LIFGLGIRHV GAKAAKILAE HFGDLPTLSR - 540
ATAEEIVALD SIGETIADSV VTYFENEEVH ELMAELEKAQ VNLTYKGLRT EQLAEVESPF - 600
KDKTVVLTGK LAQYTREEAK EKIENLGGKV TGSVSKKTDI VVAGEDAGSK LTKAESLGVT - 660
VWNEQEMVDA LDASHF
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| Functional narrative |
DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.
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| Map of ordered and disordered regions |
Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.
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| Region 1 | | Type: | Disordered | | Name: | Flexible Linker | | Location: | 68 - 76 | | Length: | 9 | | Region sequence: |
RVGGKVLSG | | Modification type: | Fragment
| | PDB: | 1TA8:A, 1TAE:A, 1TAE:B, 1TAE:C, 1TAE:D | | Structural/functional type: | Function arises from the disordered state
| | Functional classes: | Molecular assembly
Entropic chain
| | Functional subclasses: | Flexible linkers/spacers
| Detection methods:
- X-ray crystallography (110 K; )
| References:
- Gajiwala KS, Pinko C. "Structural rearrangement accompanying NAD+ synthesis within a bacterial DNA ligase crystal." Structure (Camb). 2004; 12(8): 1449-59. PubMed: 15296738
| Comments:
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