General information | DisProt: | DP00337 | Name: | Deoxyuridine 5'-triphosphate nucleotidohydrolase | Synonym(s): | DUT_ECOLI
dUTPase
EC 3.6.1.23
dUTP pyrophosphatase
| First appeared in release: | Release 3.0 (02/17/2006) | UniProt: | P06968 | UniGene: | | SwissProt: | DUT_ECOLI | TrEMBL: | | NCBI (GI): | 118953 | Source organism: | Escherichia coli | Sequence length: | 152 | Percent disordered: | 11% | Homologues: | |
Native sequence |
10 20 30 40 50 60 | | | | | | MMKKIDVKIL DPRVGKEFPL PTYATSGSAG LDLRACLNDA VELAPGDTTL VPTGLAIHIA - 60 DPSLAAMMLP RSGLGHKHGI VLGNLVGLID SDYQGQLMIS VWNRGQDSFT IQPGERIAQM - 120 IFVPVVQAEF NLVEDFDATD RGEGGFGHSG RQ
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Functional narrative |
This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.
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Map of ordered and disordered regions |
Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.
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Region 1 | Type: | Disordered | Name: | | Location: | 137 - 152 | Length: | 16 | Region sequence: |
DATDRGEGGFGHSGRQ | Modification type: | | PDB: | | Structural/functional type: | | Functional classes: | | Functional subclasses: | | Detection methods:
- X-ray crystallography (pH: 4.2; MgCl2 50 uM; PEG8000; Pyrophosphate 450 uM; Succinate buffer)
| References:
- Cedergren-Zeppezauer ES, Larsson G, Nyman PO, Dauter Z, Wilson KS. "Crystal structure of a dUTPase." Nature. 1992; 355(6362): 740-3. PubMed: 1311056
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Region 2 | Type: | Disordered | Name: | | Location: | 138 - 152 | Length: | 15 | Region sequence: |
ATDRGEGGFGHSGRQ | Modification type: | | PDB: | | Structural/functional type: | | Functional classes: | | Functional subclasses: | | Detection methods:
- Nuclear magnetic resonance (NMR) (310 K; H2O/D2O (90/10); Phosphate buffer)
| References:
- Nord J, Nyman P, Larsson G, Drakenberg T. "The C-terminus of dUTPase: observation on flexibility using NMR." FEBS Lett. 2001; 492(3): 228-32. PubMed: 11257499
| Comments:
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