Annotation for this protein is in progress - please check future releases for more complete information



DP00337: Deoxyuridine 5'-triphosphate nucleotidohydrolaseFASTA viewXML view

General information
DisProt:DP00337
Name:Deoxyuridine 5'-triphosphate nucleotidohydrolase
Synonym(s):DUT_ECOLI
dUTPase
EC 3.6.1.23
dUTP pyrophosphatase
First appeared in release:Release 3.0 (02/17/2006)
UniProt:P06968
UniGene: 
SwissProt: DUT_ECOLI
TrEMBL:  
NCBI (GI): 118953
Source organism:Escherichia coli
Sequence length:152
Percent disordered:11%
Homologues: 


Native sequence

        10         20         30         40         50         60
         |          |          |          |          |          |
MMKKIDVKIL DPRVGKEFPL PTYATSGSAG LDLRACLNDA VELAPGDTTL VPTGLAIHIA - 60
DPSLAAMMLP RSGLGHKHGI VLGNLVGLID SDYQGQLMIS VWNRGQDSFT IQPGERIAQM - 120
IFVPVVQAEF NLVEDFDATD RGEGGFGHSG RQ



Functional narrative    

This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.

Region 1: 137-152 Region 2: 138-152

Map of ordered and disordered regions







Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.


Region 1
Type:Disordered
Name: 
Location:137 - 152
Length:16
Region sequence:

DATDRGEGGFGHSGRQ

Modification type:  
PDB:  
Structural/functional type:  
Functional classes:  
Functional subclasses:  
Detection methods:
  1. X-ray crystallography (pH: 4.2; MgCl2 50 uM; PEG8000; Pyrophosphate 450 uM; Succinate buffer)

References:
  1. Cedergren-Zeppezauer ES, Larsson G, Nyman PO, Dauter Z, Wilson KS. "Crystal structure of a dUTPase." Nature. 1992; 355(6362): 740-3. PubMed: 1311056

Comments:
 



Region 2
Type:Disordered
Name: 
Location:138 - 152
Length:15
Region sequence:

ATDRGEGGFGHSGRQ

Modification type:  
PDB:  
Structural/functional type:  
Functional classes:  
Functional subclasses:  
Detection methods:
  1. Nuclear magnetic resonance (NMR) (310 K; H2O/D2O (90/10); Phosphate buffer)

References:
  1. Nord J, Nyman P, Larsson G, Drakenberg T. "The C-terminus of dUTPase: observation on flexibility using NMR." FEBS Lett. 2001; 492(3): 228-32. PubMed: 11257499

Comments:
 


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