| General information | | DisProt: | DP00344 | | Name: | Calmodulin | | Synonym(s): | CALM_DROME
CaM
| | First appeared in release: | Release 3.0 (02/17/2006) | | UniProt: | P62152 | | UniGene: | Dm.577 | | SwissProt: | CALM_DROME | | TrEMBL: | | | NCBI (GI): | 49037468 | | Source organism: | Drosophila melanogaster (Fruit fly) | | Sequence length: | 149 | | Percent disordered: | 3% | | Homologues: | |
| Native sequence |
10 20 30 40 50 60
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MADQLTEEQI AEFKEAFSLF DKDGDGTITT KELGTVMRSL GQNPTEAELQ DMINEVDADG - 60
NGTIDFPEFL TMMARKMKDT DSEEEIREAF RVFDKDGNGF ISAAELRHVM TNLGEKLTDE - 120
EVDEMIREAD IDGDGQVNYE EFVTMMTSK
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| Functional narrative |
Calmodulin mediates the control of a large number of enzymes and other proteins by Ca2+. Among the enzymes to be stimulated by the calmodulin-Ca2+ complex are a number of protein kinases and phosphatases.
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| Map of ordered and disordered regions |
Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.
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| Region 1 | | Type: | Disordered | | Name: | | | Location: | 78 - 82 | | Length: | 5 | | Region sequence: |
KDTDS | | Modification type: | Engineered
| | PDB: | | | Structural/functional type: | Function arises from the disordered state
| | Functional classes: | Entropic chain
Molecular assembly
| | Functional subclasses: | Flexible linkers/spacers
| Detection methods:
- Nuclear magnetic resonance (NMR) (pH: 6.3; CaCl2 10 mM; D2O 5 %; KCl 100 mM)
- Nuclear magnetic resonance (NMR) (308 K; pH: 6.3; CaCl2 6.1 mM; D2O 5 %; H2O 95 %; KCl 100 mM)
| References:
- Barbato G, Ikura M, Kay LE, Pastor RW, Bax A. "Backbone dynamics of calmodulin studied by 15N relaxation using inverse detected two-dimensional NMR spectroscopy: the central helix is flexible." Biochemistry. 1992; 31(23): 5269-78. PubMed: 1606151
- Brokx RD, Scheek RM, Weljie AM, Vogel HJ. "Backbone dynamic properties of the central linker region of calcium-calmodulin in 35% trifluoroethanol." J Struct Biol. 2004; 146(3): 272-80. PubMed: 15099569
| Comments:
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| Region 2 | | Type: | Disordered | | Name: | | | Location: | 79 - 82 | | Length: | 4 | | Region sequence: |
DTDS | | Modification type: | Engineered
| | PDB: | | | Structural/functional type: | Function arises from the disordered state
| | Functional classes: | Molecular assembly
Entropic chain
| | Functional subclasses: | Flexible linkers/spacers
| Detection methods:
- Nuclear magnetic resonance (NMR) (309 K; pH: 6.3; CaCl2 4.1 mM; D2O 99.9 %; KCl 100 mM)
- Nuclear magnetic resonance (NMR) (309 K; pH: 6.3; CaCl2 6.1 mM; D2O 5 %; H2O 95 %; KCl 100 mM)
| References:
- Ikura M, Spera S, Barbato G, Kay LE, Krinks M, Bax A. "Secondary structure and side-chain 1H and 13C resonance assignments of calmodulin in solution by heteronuclear multidimensional NMR spectroscopy." Biochemistry. 1991; 30(38): 9216-28. PubMed: 1909892
| Comments:The Ikura paper has an asparagine in position 129, but SwissProt assigned an aspartic acid to this position.
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| References |
- Yamanaka MK, Saugstad JA, Hanson-Painton O, McCarthy BJ, Tobin SL. "Structure and expression of the Drosophila calmodulin gene." Nucleic Acids Res. 1987; 15(8): 3335-48. PubMed: 3106931
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