| General information | | DisProt: | DP00351 | | Name: | Putative tyrosine-protein phosphatase auxilin | | Synonym(s): | AUXI_BOVIN
EC=3.1.3.48
DnaJ homolog subfamily C member 6
| | First appeared in release: | Release 3.0 (02/17/2006) | | UniProt: | Q27974 | | UniGene: | Bt.65042 | | SwissProt: | AUXI_BOVIN | | TrEMBL: | | | NCBI (GI): | 2498170 | | Source organism: | Bos taurus (Bovine) | | Sequence length: | 910 | | Percent disordered: | 29% | | Homologues: | |
| Native sequence |
10 20 30 40 50 60
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MDSSGASSPD MEPSYGGGLF DMVKGGAGRL FSNLKDNLKD TLKDTSSRVI QSVTSYTKGD - 60
LDFTYVTSRI IVMSFPLDSV DIGFRNQVDD IRSFLDSRHL DHYTVYNLSP KSYRTAKFHS - 120
RVSECSWPIR QAPSLHNLFA VCRNMYNWLL QNPKNVCVVH CLDGRAASSI LVGAMFIFCN - 180
LYSTPGPAVR LLYAKRPGIG LSPSHRRYLG YMCDLLADKP YRPHFKPLTI KSITVSPVPF - 240
FNKQRNGCRP YCDVLIGETK IYTTCADFER MKEYRVQDGK IFIPLSITVQ GDVVVSMYHL - 300
RSTIGSRLQA KVTNTQIFQL QFHTGFIPLD TTVLKFTKPE LDACDVPEKY PQLFQVTLDV - 360
ELQPHDKVME LTPPWEHYCT KDVNPSILFS SHQEHQDTLV LGGQAPIDIP PDNPRHFGQG - 420
GFFSTLCWQD QKSEKSFCEE DHAALVNQES EQSDDELLTL SSPHGNANGD KPHAARKPSK - 480
KQQEPAAPAP PEDVDLLGLE GSAVSKNFSS PAAPPSNSEL LSDLFGGGGA AGPVQSGQSG - 540
VDDVFHPSGP TSTQSTPRRS ATSTSASPTL RVGEGATFDP FGAPSKPSGQ DLLGSFLNTA - 600
SASSDPFLQP TRSPSPTVHA SSTPAVNIQP DVSGAWDWHT KPGGFGMGSK SAATSPTGSS - 660
HGTPTHQNKP QTLDPFADLG TLGGSSFASK PSTPTGLGGG FPPLSSPQKA SPQPMGGGWQ - 720
QGGGYNWQQT QSKPQSSMPH SSPQNRPNYN VSFSSMPGGQ NERGKAAANL EGKQKAADFE - 780
DLLSGQGFNA HKDKKGPRTI AEMRKEEMAK EMDPEKLKIL EWIEGKERNI RALLSTMHTV - 840
LWAGETKWKP VGMADLVTPE QVKKVYRKAV LVVHPDKATG QPYEQYAKMI FMELNDAWSE - 900
FENQGQKPLY
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| Functional narrative |
The J-domain protein auxilin is a neuron-specific protein involved in the removal of the clathrin coat from endocytosed synaptic vesicle membranes. Recruits HSPA8/HSC70 to clathrin-coated vesicles and promotes uncoating of clathrin-coated vesicles.
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| Map of ordered and disordered regions |
Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.
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| Region 1 | | Type: | Disordered | | Name: | Clathrin binding domain | | Location: | 547 - 813 | | Length: | 267 | | Region sequence: |
PSGPTSTQSTPRRSATSTSASPTLRVGEGATFDPFGAPSKPSGQDLLGSFLNTASASSDP
FLQPTRSPSPTVHASSTPAVNIQPDVSGAWDWHTKPGGFGMGSKSAATSPTGSSHGTPTH
QNKPQTLDPFADLGTLGGSSFASKPSTPTGLGGGFPPLSSPQKASPQPMGGGWQQGGGYN
WQQTQSKPQSSMPHSSPQNRPNYNVSFSSMPGGQNERGKAAANLEGKQKAADFEDLLSGQ
GFNAHKDKKGPRTIAEMRKEEMAKEMD | | Modification type: | Native
| | PDB: | | | Structural/functional type: | | | Functional classes: | | | Functional subclasses: | Protein-protein binding
| Detection methods:
- Circular dichroism (CD) spectroscopy, far-UV (75 K; PBS)
| References:
- Scheele U, Alves J, Frank R, Düwel M, Kalthoff C, Ungewickell E.
Scheele U, Alves J, Frank R, Düwel M, Kalthoff C, Ungewickell E. "Molecular and functional characterization of clathrin- and AP-2 binding determinants within a disordered domain of auxilin." J Biol Chem. 2003; 278(28): 25357-25368. PubMed: 12732633
| Comments:
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| References |
- Morgan JR, Prasad K, Jin S, Augustine GJ, Lafer EM. "Uncoating of clathrin-coated vesicles in presynaptic terminals: roles for Hsc70 and auxilin." Neuron. 2001; 32(2): 289-300. PubMed: 11683998
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