| General information | | DisProt: | DP00354 | | Name: | Fibronectin binding protein | | Synonym(s): | O50835_BORBU
Immunogenic protein P35
BBK32
| | First appeared in release: | Release 3.0 (02/17/2006) | | UniProt: | O50835 | | UniGene: | | | SwissProt: | O50835_BORBU | | TrEMBL: | | | NCBI (GI): | 2690126 | | Source organism: | Borrelia burgdorferi (strain B31)(Lyme disease spirochete) | | Sequence length: | 354 | | Percent disordered: | 42% | | Homologues: | |
| Native sequence |
10 20 30 40 50 60
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MKKVKSKYLA LGLLFGFISC DLFIRYEMKE ESPGLFDKGN SILETSEESI KKPMNKKGKG - 60
KIARKKGKSK VSRKEPYIHS LKRDSANKSN FLQKNVILEE ESLKTELLKE QSETRKEKIQ - 120
KQQDEYKGMT QGSLNSLSGE SGELEEPIES NEIDLTIDSD LRPKSSLQGI AGSNSISYTD - 180
EIEEEDYDQY YLDEYDEEDE EEIRLSNRYQ SYLEGVKYNV DSAIQTITKI YNTYTLFSTK - 240
LTQMYSTRLD NFAKAKAKEE AAKFTKEDLE KNFKTLLNYI QVSVKTAANF VYINDTHAKR - 300
KLENIEAEIK TLIAKIKEQS NLYEAYKAIV TSILLMRDSL KEVQGIIDKN GVWY
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| Functional narrative |
BBK32 is a lipoprotein located on the surface of B. burgodorferi. The N-terminal domain of BBK32 is responsible for fibronectin binding and is unstructured in the absence of the ligand.
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| Map of ordered and disordered regions |
Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.
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| Region 1 | | Type: | Disordered | | Name: | | | Location: | 56 - 205 | | Length: | 150 | | Region sequence: |
KKGKGKIARKKGKSKVSRKEPYIHSLKRDSANKSNFLQKNVILEEESLKTELLKEQSETR
KEKIQKQQDEYKGMTQGSLNSLSGESGELEEPIESNEIDLTIDSDLRPKSSLQGIAGSNS
ISYTDEIEEEDYDQYYLDEYDEEDEEEIRL | | Modification type: | Native
| | PDB: | | | Structural/functional type: | Function arises via a disorder to order transition
| | Functional classes: | Molecular assembly
| | Functional subclasses: | Protein-protein binding
| Detection methods:
- Circular dichroism (CD) spectroscopy, far-UV (296 K; pH: 7.6; )
- Size exclusion/gel filtration chromatography
- Viscometry (296 K; )
| References:
- Kim JH, Singvall J, Schwarz-Linek U, Johnson BJ, Potts JR, Hook M. "BBK32, a fibronectin binding MSCRAMM from Borrelia burgdorferi, contains a disordered region that undergoes a conformational change on ligand binding." J Biol Chem. 2004; 279(40): 41706-14. PubMed: 15292204
| Comments:
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| References |
- Kim JH, Singvall J, Schwarz-Linek U, Johnson BJ, Potts JR, Hook M. "BBK32, a fibronectin binding MSCRAMM from Borrelia burgdorferi, contains a disordered region that undergoes a conformational change on ligand binding." J Biol Chem. 2004; 279(40): 41706-14. PubMed: 15292204
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