General information | DisProt: | DP00356 | Name: | Cbp/p300-interacting transactivator 2 [Isoform 1] | Synonym(s): | CITE2_HUMAN
MSG-related protein 1
MRG-1
P35srj
| First appeared in release: | Release 3.0 (02/17/2006) | UniProt: | Q99967-1 | UniGene: | Hs.82071 | SwissProt: | CITE2_HUMAN | TrEMBL: | | NCBI (GI): | 21542403 | Source organism: | Homo sapiens (Human) | Sequence length: | 270 | Percent disordered: | 19% | Homologues: | |
Native sequence |
10 20 30 40 50 60 | | | | | | MADHMMAMNH GRFPDGTNGL HHHPAHRMGM GQFPSPHHHQ QQQPQHAFNA LMGEHIHYGA - 60 GNMNATSGIR HAMGPGTVNG GHPPSALAPA ARFNNSQFMG PPVASQGGSL PASMQLQKLN - 120 NQYFNHHPYP HNHYMPDLHP AAGHQMNGTN QHFRDCNPKH SGGSSTPGGS GGSSTPGGSG - 180 SSSGGGAGSS NSGGGSGSGN MPASVAHVPA AMLPPNVIDT DFIDEEVLMS LVIEMGLDRI - 240 KELPELWLGQ NEFDFMTDFV CKQQPSRVSC
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Functional narrative |
Cbp/p300-interacting transactivator 2 is a ubiquitously expressed nuclear protein that competes with HIF-1 alpha for binding to the CBP/p300 TAZ1 domain. Expression is directly induced by HIF-1, implicating the protein as a negative feedback regulator of HIF-1 alpha and a fundamental component of the cellular mechanism for attenuation of the hypoxic response. The activation domain is unstructured when free and folds upon binding, forming a helix (termed alpha A) and an extended structure that wraps around TAZ1.
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Map of ordered and disordered regions |
Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.
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Region 1 | Type: | Disordered | Name: | Activation domain | Location: | 220 - 270 | Length: | 51 | Region sequence: |
TDFIDEEVLMSLVIEMGLDRIKELPELWLGQNEFDFMTDFVCKQQPSRVSC | Modification type: | Native
| PDB: | | Structural/functional type: | Function arises via a disorder to order transition | Functional classes: | Molecular assembly
| Functional subclasses: | Transactivation (transcriptional activation)
Protein-protein binding
| Detection methods:
- Circular dichroism (CD) spectroscopy, far-UV
- Nuclear magnetic resonance (NMR)
| References:
- De Guzman RN, Martinez-Yamout MA, Dyson HJ, Wright PE. "Interaction of the TAZ1 domain of the CREB-binding protein with the activation domain of CITED2: regulation by competition between intrinsically unstructured ligands for non-identical binding sites." J Biol Chem. 2004; 279(4): 3042-9. PubMed: 14594809
| Comments:
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References |
- De Guzman RN, Martinez-Yamout MA, Dyson HJ, Wright PE. "Interaction of the TAZ1 domain of the CREB-binding protein with the activation domain of CITED2: regulation by competition between intrinsically unstructured ligands for non-identical binding sites." J Biol Chem. 2004; 279(4): 3042-9. PubMed: 14594809
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