| General information | | DisProt: | DP00375 | | Name: | Endonuclease VIII | | Synonym(s): | END8_ECOLI
DNA glycosylase/AP lyase Nei
EC 4.2.99.18
DNA-(apurinic or apyrimidinic site) lyase Nei
| | First appeared in release: | Release 3.0 (02/17/2006) | | UniProt: | P50465 | | UniGene: | | | SwissProt: | END8_ECOLI | | TrEMBL: | | | NCBI (GI): | 1706651 | | Source organism: | Escherichia coli | | Sequence length: | 263 | | Percent disordered: | 13% | | Homologues: | |
| Native sequence |
10 20 30 40 50 60
| | | | | |
MPEGPEIRRA ADNLEAAIKG KPLTDVWFAF PQLKPYQSQL IGQHVTHVET RGKALLTHFS - 60
NDLTLYSHNQ LYGVWRVVDT GEEPQTTRVL RVKLQTADKT ILLYSASDIE MLTPEQLTTH - 120
PFLQRVGPDV LDPNLTPEVV KERLLSPRFR NRQFAGLLLD QAFLAGLGNY LRVEILWQVG - 180
LTGNHKAKDL NAAQLDALAH ALLEIPRFSY ATRGQVDENK HHGALFRFKV FHRDGEPCER - 240
CGSIIEKTTL SSRPFYWCPG CQH
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| Functional narrative |
Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, 5,6-dihydrouracil and 5,6-dihydrothymine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates.
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| Map of ordered and disordered regions |
Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.
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| Region 1 | | Type: | Disordered | | Name: | | | Location: | 122 - 128 | | Length: | 7 | | Region sequence: |
FLQRVGP | | Modification type: | Mutant
| | PDB: | | | Structural/functional type: | Function arises from the disordered state
| | Functional classes: | Entropic chain
Molecular assembly
| | Functional subclasses: | Flexible linkers/spacers
| Detection methods:
- X-ray crystallography (MgCl2 0.2 M; PEG 400 35 %; Tris-HCl (pH 8.4) 0.1 M)
| References:
- Golan G, Zharkov DO, Feinberg H, Fernandes AS, Zaika EI, Kycia JH, Grollman AP, Shoham G. "Structure of the uncomplexed DNA repair enzyme endonuclease VIII indicates significant interdomain flexibility." Nucleic Acids Res. 2005; 33(15): 5006-16. PubMed: 16145054
| Comments:Mutant Nei-R252A, which has an A in place of R at residue 252 was used to determine this structure.
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| Region 2 | | Type: | Disordered | | Name: | | | Location: | 2 - 5 | | Length: | 4 | | Region sequence: |
PEGP | | Modification type: | Mutant
| | PDB: | | | Structural/functional type: | Function arises via a disorder to order transition
| | Functional classes: | Unknown
| | Functional subclasses: | Unknown
| Detection methods:
- X-ray crystallography (MgCl2 0.2 M; PEG 400 35 %; Tris-HCl (pH 8.4) 0.1 M)
| References:
- Golan G, Zharkov DO, Feinberg H, Fernandes AS, Zaika EI, Kycia JH, Grollman AP, Shoham G. "Structure of the uncomplexed DNA repair enzyme endonuclease VIII indicates significant interdomain flexibility." Nucleic Acids Res. 2005; 33(15): 5006-16. PubMed: 16145054
| Comments:Mutant Nei-R252A, which has an A in place of R at residue 252 was used to determine this structure.
|
| Region 3 | | Type: | Disordered | | Name: | zinc finger motif | | Location: | 246 - 257 | | Length: | 12 | | Region sequence: |
EKTTLSSRPFYW | | Modification type: | Mutant
| | PDB: | | | Structural/functional type: | Function arises via a disorder to order transition
| | Functional classes: | Unknown
| | Functional subclasses: | Unknown
| Detection methods:
- X-ray crystallography (MgCl2 0.2 M; PEG 400 35 %; Tris-HCl (pH 8.4) 0.1 M)
| References:
- Golan G, Zharkov DO, Feinberg H, Fernandes AS, Zaika EI, Kycia JH, Grollman AP, Shoham G. "Structure of the uncomplexed DNA repair enzyme endonuclease VIII indicates significant interdomain flexibility." Nucleic Acids Res. 2005; 33(15): 5006-16. PubMed: 16145054
| Comments:Mutant Nei-R252A, which has an A in place of R at residue 252 was used to determine this structure.
|
| Region 4 | | Type: | Disordered | | Name: | | | Location: | 214 - 224 | | Length: | 11 | | Region sequence: |
GQVDENKHHGA | | Modification type: | Complex
Mutant
| | PDB: | | | Structural/functional type: | Function arises via an order to disorder transition
| | Functional classes: | Molecular assembly
| | Functional subclasses: | Protein-DNA binding
| Detection methods:
- X-ray crystallography (MgCl2 0.2 M; PEG 400 35 %; Tris-HCl (pH 8.4) 0.1 M)
| References:
- Golan G, Zharkov DO, Feinberg H, Fernandes AS, Zaika EI, Kycia JH, Grollman AP, Shoham G. "Structure of the uncomplexed DNA repair enzyme endonuclease VIII indicates significant interdomain flexibility." Nucleic Acids Res. 2005; 33(15): 5006-16. PubMed: 16145054
| Comments:Mutant Nei-R252A, which has an A in place of R at residue 252 was used to determine this structure.
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