| General information | | DisProt: | DP00376 | | Name: | Zinc finger protein Eos [Isoform 1] | | Synonym(s): | IKZF4_HUMAN
Ikaros family zinc finger protein 4
| | First appeared in release: | Release 3.0 (02/17/2006) | | UniProt: | Q9H2S9-1 | | UniGene: | Hs.553221 | | SwissProt: | IKZF4_HUMAN | | TrEMBL: | | | NCBI (GI): | 158564025 | | Source organism: | Homo sapiens (Human) | | Sequence length: | 585 | | Percent disordered: | 4% | | Homologues: | |
| Native sequence |
10 20 30 40 50 60
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MHTPPALPRR FQGGGRVRTP GSHRQGKDNL ERDPSGGCVP DFLPQAQDSN HFIMESLFCE - 60
SSGDSSLEKE FLGAPVGPSV STPNSQHSSP SRSLSANSIK VEMYSDEESS RLLGPDERLL - 120
EKDDSVIVED SLSEPLGYCD GSGPEPHSPG GIRLPNGKLK CDVCGMVCIG PNVLMVHKRS - 180
HTGERPFHCN QCGASFTQKG NLLRHIKLHS GEKPFKCPFC NYACRRRDAL TGHLRTHSVS - 240
SPTVGKPYKC NYCGRSYKQQ STLEEHKERC HNYLQSLSTE AQALAGQPGD EIRDLEMVPD - 300
SMLHSSSERP TFIDRLANSL TKRKRSTPQK FVGEKQMRFS LSDLPYDVNS GGYEKDVELV - 360
AHHSLEPGFG SSLAFVGAEH LRPLRLPPTN CISELTPVIS SVYTQMQPLP GRLELPGSRE - 420
AGEGPEDLAD GGPLLYRPRG PLTDPGASPS NGCQDSTDTE SNHEDRVAGV VSLPQGPPPQ - 480
PPPTIVVGRH SPAYAKEDPK PQEGLLRGTP GPSKEVLRVV GESGEPVKAF KCEHCRILFL - 540
DHVMFTIHMG CHGFRDPFEC NICGYHSQDR YEFSSHIVRG EHKVG
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| Functional narrative |
DNA-binding protein that binds to the 5'GGGAATRCC-3' Ikaros-binding sequence. Transcriptional repressor. Interacts with SPI1 and MITF to repress transcription of the CTSK and ACP5 promoters via recruitment of corepressors SIN3A and CTBP2. May be involved in the development of central and peripheral nervous systems.
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| Map of ordered and disordered regions |
Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.
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| Region 1 | | Type: | Disordered | | Name: | Finger C2 | | Location: | 560 - 582 | | Length: | 23 | | Region sequence: |
CNICGYHSQDRYEFSSHIVRGEH | | Modification type: | Engineered
Fragment
Monomeric
| | PDB: | | | Structural/functional type: | Function arises via a disorder to order transition
| | Functional classes: | Molecular assembly
| | Functional subclasses: | Substrate/ligand binding
| Detection methods:
- Circular dichroism (CD) spectroscopy, far-UV (293 K; )
| References:
- Westman BJ, Perdomo J, Matthews JM, Crossley M, Mackay JP. "Structural studies on a protein-binding zinc-finger domain of Eos reveal both similarities and differences to classical zinc fingers." Biochemistry. 2004; 43(42): 13318-27. PubMed: 15491138
| Comments:
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| Region 2 | | Type: | Disordered | | Name: | | | Location: | 576 - 582 | | Length: | 7 | | Region sequence: |
HIVRGEH | | Modification type: | Complex
Engineered
Fragment
Monomeric
| | PDB: | | | Structural/functional type: | Function arises via a disorder to order transition
| | Functional classes: | Molecular assembly
| | Functional subclasses: | Substrate/ligand binding
| Detection methods:
- Nuclear magnetic resonance (NMR) (pH: 5.3; TCEP 100 uM; ZnSO4 200 uM)
- Nuclear magnetic resonance (NMR) (pH: 6.9; TCEP 100 uM; ZnSO4 200 uM)
| References:
- Westman BJ, Perdomo J, Matthews JM, Crossley M, Mackay JP. "Structural studies on a protein-binding zinc-finger domain of Eos reveal both similarities and differences to classical zinc fingers." Biochemistry. 2004; 43(42): 13318-27. PubMed: 15491138
| Comments:
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