| General information | | DisProt: | DP00396 | | Name: | Eukaryotic peptide chain release factor GTP-binding subunit | | Synonym(s): | ERF3_SCHPO
ERF2
ERF-3
ERF3
Translation release factor 3
Polypeptide release factor 3
| | First appeared in release: | Release 3.0 (02/17/2006) | | UniProt: | O74718 | | UniGene: | | | SwissProt: | ERF3_SCHPO | | TrEMBL: | | | NCBI (GI): | 7404356 | | Source organism: | Schizosaccharomyces pombe (Fission yeast) | | Sequence length: | 662 | | Percent disordered: | 9% | | Homologues: | |
| Native sequence |
10 20 30 40 50 60
| | | | | |
MASNQPNNGE QDEQLAKQTS KLSMSAKAPT FTPKAAPFIP SFQRPGFVPV NNIAGGYPYA - 60
QYTGQGQNSN SPHPTKSYQQ YYQKPTGNTV DEDKSRVPDF SKKKSFVPPK PAIPKGKVLS - 120
LGGNTSAPKS TKPISISLGG TKAPTTTKPA APAAQSKTET PAPKVTSEST KKETAAPPPQ - 180
ETPTKSADAE LAKTPSAPAA ALKKAAEAAE PATVTEDATD LQNEVDQELL KDMYGKEHVN - 240
IVFIGHVDAG KSTLGGNILF LTGMVDKRTM EKIEREAKEA GKESWYLSWA LDSTSEEREK - 300
GKTVEVGRAY FETEHRRFSL LDAPGHKGYV TNMINGASQA DIGVLVISAR RGEFEAGFER - 360
GGQTREHAVL ARTQGINHLV VVINKMDEPS VQWSEERYKE CVDKLSMFLR RVAGYNSKTD - 420
VKYMPVSAYT GQNVKDRVDS SVCPWYQGPS LLEYLDSMTH LERKVNAPFI MPIASKYKDL - 480
GTILEGKIEA GSIKKNSNVL VMPINQTLEV TAIYDEADEE ISSSICGDQV RLRVRGDDSD - 540
VQTGYVLTST KNPVHATTRF IAQIAILELP SILTTGYSCV MHIHTAVEEV SFAKLLHKLD - 600
KTNRKSKKPP MFATKGMKII AELETQTPVC MERFEDYQYM GRFTLRDQGT TVAVGKVVKI - 660
LD
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| Functional narrative |
Involved in translation termination. Stimulates the activity of ERF1. Binds guanine nucleotides.
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| Map of ordered and disordered regions |
Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.
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| Region 1 | | Type: | Disordered | | Name: | | | Location: | 196 - 214 | | Length: | 19 | | Region sequence: |
SAPAAALKKAAEAAEPATV | | Modification type: | Fragment
| | PDB: | | | Structural/functional type: | Relationship to function unknown
| | Functional classes: | Unknown
| | Functional subclasses: | Unknown
| Detection methods:
- X-ray crystallography (277 K; DTT 10 mM; Ethylene glycol (18%); Glycerol (5%); HEPES (pH 7.5) 100 mM; NaCl 150 mM; PEG 8000 (2-8%))
- X-ray crystallography (277 K; DTT 10 mM; Ethylene glycol (18%); Glycerol (5%); HEPES (pH 7.5) 100 mM; NaCl 150 mM; PEG 8000 (2-8%); GDP 1 mM; Mg2+ 2 mM)
- X-ray crystallography (277 K; DTT 10 mM; Ethylene glycol (18%); GDPNP 1 mM; Glycerol (5%); HEPES (pH 7.5) 100 mM; Mg2+ 2 mM; NaCl 150 mM; PEG 8000 (2-8%))
| References:
- Kong C, Ito K, Walsh MA, Wada M, Liu Y, Kumar S, Barford D, Nakamura Y, Song H. "Crystal structure and functional analysis of the eukaryotic class II release factor eRF3 from S. pombe." Mol Cell. 2004; 14(2): 233-45. PubMed: 15099522
| Comments:The fragment used for structure determination consisted of residues 196-662.
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| Region 2 | | Type: | Disordered | | Name: | | | Location: | 280 - 307 | | Length: | 28 | | Region sequence: |
AGKESWYLSWALDSTSEEREKGKTVEVG | | Modification type: | Fragment
| | PDB: | | | Structural/functional type: | Function arises via a disorder to order transition
| | Functional classes: | Unknown
| | Functional subclasses: | Unknown
| Detection methods:
- X-ray crystallography (277 K; DTT 10 mM; Ethylene glycol (12-18%); Glycerol (5%); HEPES (pH 7.5) 100 mM; NaCl 150 mM; PEG 8000 (2-8%))
- X-ray crystallography (277 K; DTT 10 mM; Ethlyene glycol (12-18%); GDP 1 mM; Glycerol (5%); HEPES (pH 7.5) 100 mM; Mg2+ 2 mM; NaCl 150 mM; PEG 8000 (2-8%))
- X-ray crystallography (277 K; DTT 10 mM; Ethylene glycol (12-18%); GDPNP 1 mM; Glycerol (5%); HEPES (pH 7.5) 100 mM; Mg2+ 2 mM; NaCl 150 mM; PEG 8000 (2-8%))
| References:
- Kong C, Ito K, Walsh MA, Wada M, Liu Y, Kumar S, Barford D, Nakamura Y, Song H. "Crystal structure and functional analysis of the eukaryotic class II release factor eRF3 from S. pombe." Mol Cell. 2004; 14(2): 233-45. PubMed: 15099522
| Comments:The fragment used for structure determination consisted of residues 196-662.
Disordered region 2 is contained within Switch I of the protein.
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| Region 3 | | Type: | Disordered | | Name: | | | Location: | 325 - 335 | | Length: | 11 | | Region sequence: |
GHKGYVTNMIN | | Modification type: | Fragment
| | PDB: | | | Structural/functional type: | Function arises via a disorder to order transition
| | Functional classes: | Unknown
| | Functional subclasses: | Unknown
| Detection methods:
- X-ray crystallography (277 K; HEPES (pH 7.5) 100 mM; PEG 8000 (2-8%); Ethylene glycol (12-18%); DTT 10 mM; Glycerol (5%); NaCl 150 mM)
- X-ray crystallography (277 K; DTT 10 mM; Ethylene glycol (12-18%); GDP 1 mM; Glycerol (5%); HEPES (pH 7.5) 100 mM; Mg2+ 2 mM; NaCl 150 mM; PEG 8000 (2-8%))
- X-ray crystallography (277 K; DTT 10 mM; Ethylene glycol (12-18%); GDPNP 1 mM; Glycerol (5%); HEPES (pH 7.5) 100 mM; Mg2+ 2 mM; NaCl 150 mM; PEG 8000 (2-8%))
| References:
- Kong C, Ito K, Walsh MA, Wada M, Liu Y, Kumar S, Barford D, Nakamura Y, Song H. "Crystal structure and functional analysis of the eukaryotic class II release factor eRF3 from S. pombe." Mol Cell. 2004; 14(2): 233-45. PubMed: 15099522
| Comments:The fragment used for structure determination consisted of residues 196-662.
Disordered region 3 is contained within Switch II of the protein.
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