| General information | | DisProt: | DP00421 | | Name: | Protein phosphatase 1 regulatory subunit 1B | | Synonym(s): | PPR1B_BOVIN
Dopamine- and cAMP-regulated neuronal phosphoprotein
DARPP-32
| | First appeared in release: | Release 3.0 (02/17/2006) | | UniProt: | P07516 | | UniGene: | Bt.4706 | | SwissProt: | PPR1B_BOVIN | | TrEMBL: | | | NCBI (GI): | 124837 | | Source organism: | Bos taurus (Bovine) | | Sequence length: | 202 | | Percent disordered: | 100% | | Homologues: | |
| Native sequence |
10 20 30 40 50 60
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MDPKDRKKIQ FSVPAPPSQL DPRQVEMIRR RRPTPAMLFR LSEHSSPEEE ASPHQRASGE - 60
GHHLKSKRSN PCAYTPPSLK AVQRIAESHL QSISNLGENQ ASEEEDELGE LRELGYPREE - 120
EEEEEEEDEE EEEDSQAEVL KGSRGSAGQK TTYGQGLEGP WERPPPLDGP QRDGSSEDQV - 180
EDPALNEPGE EPQRPAHPEP GT
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| Functional narrative |
DARPP-32 is a messenger that integrates multiple signaling pathways in the brain. By its ability to modulate the activity of PP-1 and PKA, DARPP-32 is critically involved in regulating electrophysiological, transcriptional, and behavioral responses to physiological and pharmacological stimuli. Inhibitor of protein-phosphatase 1.
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| Map of ordered and disordered regions |
Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.
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| Region 1 | | Type: | Disordered | | Name: | | | Location: | 1 - 202 | | Length: | 202 | | Region sequence: |
MDPKDRKKIQFSVPAPPSQLDPRQVEMIRRRRPTPAMLFRLSEHSSPEEEASPHQRASGE
GHHLKSKRSNPCAYTPPSLKAVQRIAESHLQSISNLGENQASEEEDELGELRELGYPREE
EEEEEEEDEEEEEDSQAEVLKGSRGSAGQKTTYGQGLEGPWERPPPLDGPQRDGSSEDQV
EDPALNEPGEEPQRPAHPEPGT | | Modification type: | Native
| | PDB: | | | Structural/functional type: | | | Functional classes: | | | Functional subclasses: | Protein-protein binding
| Detection methods:
- SDS-PAGE gel, Aberrant mobility on
- Size exclusion/gel filtration chromatography (277 K; pH: 7.4; 2-mercaptoethanol 15 mM; EDTA 2 mM; Isopropanol 0.1 %; NaCl 200 mM; PMSF 0.1 mM; Potassium phosphate 10 mM; Sodium azide 0.02 %)
- Analytical ultracentrifugation (277 K; pH: 7.6; 2-mercaptoethanol 1.5 mM; EDTA 0.2 mM; NaCl 50 mM; Tris-HCl 50 mM)
| References:
- Hemmings HC Jr, Nairn AC, Aswad DW, Greengard P. "DARPP-32, a dopamine- and adenosine 3':5'-monophosphate-regulated phosphoprotein enriched in dopamine-innervated brain regions. II. Purification and characterization of the phosphoprotein from bovine caudate nucleus." J Neurosci. 1984; 4(1): 99-110. PubMed: 6319628
| Comments:
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| References |
- Svenningsson P, Nishi A, Fisone G, Girault JA, Nairn AC, Greengard P. "DARPP-32: an integrator of neurotransmission." Annu Rev Pharmacol Toxicol. 2004; 44: 269-96. PubMed: 14744247
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