| General information | | DisProt: | DP00472 | | Name: | Peroxisomal targeting signal 1 receptor [Isoform 3] | | Synonym(s): | PEX5_HUMAN
PTS1 receptor
PTS1R
Peroxisome receptor 1
Peroxisomal C-terminal targeting signal import receptor
PTS1-BP
Peroxin-5
| | First appeared in release: | Release 3.3 (06/23/2006) | | UniProt: | P50542-3 | | UniGene: | Hs.567327 | | SwissProt: | PEX5_HUMAN | | TrEMBL: | | | NCBI (GI): | 119364633 | | Source organism: | Homo sapiens (Human) | | Sequence length: | 631 | | Percent disordered: | 51% | | Homologues: | |
| Native sequence |
10 20 30 40 50 60
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MAMRELVEAE CGGANPLMKL AGHFTQDKAL RQEGLRPGPW PPGAPASEAA SKPLGVASED - 60
ELVAEFLQDQ NAPLVSRAPQ TFKMDDLLAE MQQIEQSNFR QAPQRAPGVA DLALSENWAQ - 120
EFLAAGDAVD VTQDYNETDW SQEFISEVTD PLSVSPARWA EEYLEQSEEK LWLGEPEGTA - 180
TDRWYDEYHP EEDLQHTASD FVAKVDDPKL ANSEFLKFVR QIGEGQVSLE SGAGSGRAQA - 240
EQWAAEFIQQ QGTSDAWVDQ FTRPVNTSAL DMEFERAKSA IELQAELEEM AKRDAEAHPW - 300
LSDYDDLTSA TYDKGYQFEE ENPLRDHPQP FEEGLRRLQE GDLPNAVLLF EAAVQQDPKH - 360
MEAWQYLGTT QAENEQELLA ISALRRCLEL KPDNQTALMA LAVSFTNESL QRQACETLRD - 420
WLRYTPAYAH LVTPAEEGAG GAGLGPSKRI LGSLLSDSLF LEVKELFLAA VRLDPTSIDP - 480
DVQCGLGVLF NLSGEYDKAV DCFTAALSVR PNDYLLWNKL GATLANGNQS EEAVAAYRRA - 540
LELQPGYIRS RYNLGISCIN LGAHREAVEH FLEALNMQRK SRGPRGEGGA MSENIWSTLR - 600
LALSMLGQSD AYGAADARDL STLLTMFGLP Q
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| Functional narrative |
Peroxisomal matrix proteins are synthesized in cytosolic ribosomes and post-translationally imported into the organelle. Two soluble receptor proteins, Pex5p and Pex7p, promote the specific targeting of these newly synthesized proteins into the peroxisome. By far the majority of peroxisomal matrix proteins use Pex5p as their cognate receptor. These proteins possess the so-called peroxisomal targeting signal (PTS) type 1, a tripeptide located at their extreme C terminus with the sequence S-K-L or a variant. The C-terminal half of Pex5p, a region containing several TPR motifs, interacts with the PTS1 sequence. Binds to the C-terminal PTS1-type tripeptide peroxisomal targeting signal (SKL-type) and plays an essential role in peroxisomal protein import.
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| Map of ordered and disordered regions |
Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.
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| Region 1 | | Type: | Disordered - Pre-Molten Globule | | Name: | | | Location: | 1 - 324 | | Length: | 324 | | Region sequence: |
MAMRELVEAECGGANPLMKLAGHFTQDKALRQEGLRPGPWPPGAPASEAASKPLGVASED
ELVAEFLQDQNAPLVSRAPQTFKMDDLLAEMQQIEQSNFRQAPQRAPGVADLALSENWAQ
EFLAAGDAVDVTQDYNETDWSQEFISEVTDPLSVSPARWAEEYLEQSEEKLWLGEPEGTA
TDRWYDEYHPEEDLQHTASDFVAKVDDPKLANSEFLKFVRQIGEGQVSLESGAGSGRAQA
EQWAAEFIQQQGTSDAWVDQFTRPVNTSALDMEFERAKSAIELQAELEEMAKRDAEAHPW
LSDYDDLTSATYDKGYQFEEENPL | | Modification type: | Native
| | PDB: | | | Structural/functional type: | Function arises from the pre-molten globule state
| | Functional classes: | Molecular assembly
Chaperones
| | Functional subclasses: | Self-transport through channel
Protein-protein binding
| Detection methods:
- Circular dichroism (CD) spectroscopy, far-UV (298 K; pH: 7.5; Sodium phosphate 10 mM)
- Size exclusion/gel filtration chromatography (298 K; pH: 7.5; DTT 1 mM; EDTA-NaOH 1 mM; NaCl 150 mM; Tris-HCl 50 mM)
- Fourier transform infrared spectroscopy (FTIR), aka infrared spectroscopy) (298 K; pH: 7.5; NaCl 150 mM; protein 1 mg/ml; Tris-HCl 50 mM)
- Sensitivity to proteolysis (273 K; 0.125 microg/ml - 1 microg/ml proteinase K; 5 micrograms protein)
- Stability at thermal extremes (368 K; 30 min)
| References:
- Carvalho AF, Costa-Rodrigues J, Correia I, Costa Pessoa J, Faria TQ, Martins CL, Fransen M, Sa-Miranda C, Azevedo JE. "The N-terminal half of the peroxisomal cycling receptor Pex5p is a natively unfolded domain." J Mol Biol. 2006; 356(4): 864-75. PubMed: 16403517
- Dodt G, Gould SJ. "Multiple PEX genes are required for proper subcellular distribution and stability of Pex5p, the PTS1 receptor: evidence that PTS1 protein import is mediated by a cycling receptor." J Cell Biol. 1996; 135(6 Pt 2): 1763-74. PubMed: 8991089
- Otera H, Setoguchi K, Hamasaki M, Kumashiro T, Shimizu N, Fujiki Y. "Peroxisomal targeting signal receptor Pex5p interacts with cargoes and import machinery components in a spatiotemporally differentiated manner: conserved Pex5p WXXXF/Y motifs are critical for matrix protein import." Mol Cell Biol. 2002; 22(6): 1639-55. PubMed: 11865044
| Comments:
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| References |
- Purdue PE, Lazarow PB. "Peroxisome biogenesis." Annu Rev Cell Dev Biol. 2001; 17: 701-52. PubMed: 11687502
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