| General information | | DisProt: | DP00487 | | Name: | Ribonucleoside-diphosphate reductase small chain 1 | | Synonym(s): | RIR2_YEAST
EC=1.17.4.1
Ribonucleotide reductase small subunit 1
Ribonucleotide reductase R2 subunit 1
| | First appeared in release: | Release 3.2 (05/26/2006) | | UniProt: | P09938 | | UniGene: | | | SwissProt: | RIR2_YEAST | | TrEMBL: | | | NCBI (GI): | 1172946 | | Source organism: | Saccharomyces cerevisiae (Baker's yeast) | | Sequence length: | 399 | | Percent disordered: | 17% | | Homologues: | |
| Native sequence |
10 20 30 40 50 60
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MPKETPSKAA ADALSDLEIK DSKSNLNKEL ETLREENRVK SDMLKEKLSK DAENHKAYLK - 60
SHQVHRHKLK EMEKEEPLLN EDKERTVLFP IKYHEIWQAY KRAEASFWTA EEIDLSKDIH - 120
DWNNRMNENE RFFISRVLAF FAASDGIVNE NLVENFSTEV QIPEAKSFYG FQIMIENIHS - 180
ETYSLLIDTY IKDPKESEFL FNAIHTIPEI GEKAEWALRW IQDADALFGE RLVAFASIEG - 240
VFFSGSFASI FWLKKRGMMP GLTFSNELIC RDEGLHTDFA CLLFAHLKNK PDPAIVEKIV - 300
TEAVEIEQRY FLDALPVALL GMNADLMNQY VEFVADRLLV AFGNKKYYKV ENPFDFMENI - 360
SLAGKTNFFE KRVSDYQKAG VMSKSTKQEA GAFTFNEDF
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| Functional narrative |
Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. RNR2 provides the diiron-tyrosyl radical center.
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| Map of ordered and disordered regions |
Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.
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| Region 1 | | Type: | Disordered | | Name: | | | Location: | 1 - 25 | | Length: | 25 | | Region sequence: |
MPKETPSKAAADALSDLEIKDSKSN | | Modification type: | Complex
Engineered
| | PDB: | | | Structural/functional type: | Relationship to function unknown
| | Functional classes: | Unknown
| | Functional subclasses: | Unknown
| Detection methods:
- X-ray crystallography (acetate buffer (pH 4.9) 100 mM; NaCl 200 mM; PEG 4000 (14% wt/vol))
| References:
- Voegtli WC, Ge J, Perlstein DL, Stubbe J, Rosenzweig AC. "Structure of the yeast ribonucleotide reductase Y2Y4 heterodimer." Proc Natl Acad Sci U S A. 2001; 98(18): 10073-8. PubMed: 11526233
| Comments:
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| Region 2 | | Type: | Disordered | | Name: | | | Location: | 146 - 148 | | Length: | 3 | | Region sequence: |
GIV | | Modification type: | Complex
Engineered
| | PDB: | | | Structural/functional type: | Relationship to function unknown
| | Functional classes: | Unknown
| | Functional subclasses: | Unknown
| Detection methods:
- X-ray crystallography (acetate buffer (pH 4.9) 100 mM; NaCl 200 mM; PEG 4000 (14% wt/vol))
| References:
- Voegtli WC, Ge J, Perlstein DL, Stubbe J, Rosenzweig AC. "Structure of the yeast ribonucleotide reductase Y2Y4 heterodimer." Proc Natl Acad Sci U S A. 2001; 98(18): 10073-8. PubMed: 11526233
| Comments:
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| Region 3 | | Type: | Disordered | | Name: | | | Location: | 360 - 399 | | Length: | 40 | | Region sequence: |
ISLAGKTNFFEKRVSDYQKAGVMSKSTKQEAGAFTFNEDF | | Modification type: | Complex
Engineered
| | PDB: | | | Structural/functional type: | Relationship to function unknown
| | Functional classes: | Unknown
| | Functional subclasses: | Protein-protein binding
| Detection methods:
- X-ray crystallography (acetate buffer (pH 4.9) 100 mM; NaCl 200 mM; PEG 4000 (14% wt/vol))
| References:
- Voegtli WC, Ge J, Perlstein DL, Stubbe J, Rosenzweig AC. "Structure of the yeast ribonucleotide reductase Y2Y4 heterodimer." Proc Natl Acad Sci U S A. 2001; 98(18): 10073-8. PubMed: 11526233
| Comments:
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| Comments |
Yeast has two R2 subunits (RNR2 and RNR4 or Y2 and Y4). Y2 provides the iron-radical center and Y4 is required in the assembly of the diferric-Y cofactor in Y2. Refer to DP00488 for RNR4 or Y4.
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