General information | DisProt: | DP00488 | Name: | Ribonucleoside-diphosphate reductase small chain 2 | Synonym(s): | RIR4_YEAST
EC=1.17.4.1
Ribonucleotide reductase small subunit 2
Ribonucleotide reductase R2 subunit 2
| First appeared in release: | Release 3.2 (05/26/2006) | UniProt: | P49723 | UniGene: | | SwissProt: | RIR4_YEAST | TrEMBL: | | NCBI (GI): | 1710402 | Source organism: | Saccharomyces cerevisiae (Baker's yeast) | Sequence length: | 345 | Percent disordered: | 23% | Homologues: | |
Native sequence |
10 20 30 40 50 60 | | | | | | MEAHNQFLKT FQKERHDMKE AEKDEILLME NSRRFVMFPI KYHEIWAAYK KVEASFWTAE - 60 EIELAKDTED FQKLTDDQKT YIGNLLALSI SSDNLVNKYL IENFSAQLQN PEGKSFYGFQ - 120 IMMENIYSEV YSMMVDAFFK DPKNIPLFKE IANLPEVKHK AAFIERWISN DDSLYAERLV - 180 AFAAKEGIFQ AGNYASMFWL TDKKIMPGLA MANRNICRDR GAYTDFSCLL FAHLRTKPNP - 240 KIIEKIITEA VEIEKEYYSN SLPVEKFGMD LKSIHTYIEF VADGLLQGFG NEKYYNAVNP - 300 FEFMEDVATA GKTTFFEKKV SDYQKASDMS KSATPSKEIN FDDDF
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Functional narrative |
Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. RNR4 is required for proper folding of RNR2 and assembly with the large subunits.
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Map of ordered and disordered regions |
Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.
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Region 1 | Type: | Disordered | Name: | | Location: | 1 - 10 | Length: | 10 | Region sequence: |
MEAHNQFLKT | Modification type: | Complex
| PDB: | | Structural/functional type: | Relationship to function unknown | Functional classes: | Unknown
| Functional subclasses: | Unknown
| Detection methods:
- X-ray crystallography (acetate buffer (pH 4.9) 100 mM; NaCl 200 mM; PEG 4000 (14% wt/vol))
| References:
- Voegtli WC, Ge J, Perlstein DL, Stubbe J, Rosenzweig AC. "Structure of the yeast ribonucleotide reductase Y2Y4 heterodimer." Proc Natl Acad Sci U S A. 2001; 98(18): 10073-8. PubMed: 11526233
| Comments:
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Region 2 | Type: | Disordered | Name: | | Location: | 91 - 99 | Length: | 9 | Region sequence: |
SSDNLVNKY | Modification type: | Complex
| PDB: | | Structural/functional type: | Relationship to function unknown | Functional classes: | Unknown
| Functional subclasses: | Unknown
| Detection methods:
- X-ray crystallography (acetate buffer (pH 4.9) 100 mM; NaCl 200 mM; PEG 4000 (14% wt/vol))
| References:
- Voegtli WC, Ge J, Perlstein DL, Stubbe J, Rosenzweig AC. "Structure of the yeast ribonucleotide reductase Y2Y4 heterodimer." Proc Natl Acad Sci U S A. 2001; 98(18): 10073-8. PubMed: 11526233
| Comments:
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Region 3 | Type: | Disordered | Name: | | Location: | 264 - 274 | Length: | 11 | Region sequence: |
VEKFGMDLKSI | Modification type: | Complex
| PDB: | | Structural/functional type: | Relationship to function unknown | Functional classes: | Unknown
| Functional subclasses: | Unknown
| Detection methods:
- X-ray crystallography (acetate buffer (pH 4.9) 100 mM; NaCl 200 mM; PEG 4000 (14% wt/vol))
| References:
- Voegtli WC, Ge J, Perlstein DL, Stubbe J, Rosenzweig AC. "Structure of the yeast ribonucleotide reductase Y2Y4 heterodimer." Proc Natl Acad Sci U S A. 2001; 98(18): 10073-8. PubMed: 11526233
| Comments:
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Region 4 | Type: | Disordered | Name: | | Location: | 295 - 345 | Length: | 51 | Region sequence: |
YNAVNPFEFMEDVATAGKTTFFEKKVSDYQKASDMSKSATPSKEINFDDDF | Modification type: | Complex
| PDB: | | Structural/functional type: | Relationship to function unknown | Functional classes: | Unknown
| Functional subclasses: | Protein-protein binding
| Detection methods:
- X-ray crystallography (acetate buffer (pH 4.9) 100 mM; NaCl 200 mM; PEG 4000 (14% wt/vol))
| References:
- Voegtli WC, Ge J, Perlstein DL, Stubbe J, Rosenzweig AC. "Structure of the yeast ribonucleotide reductase Y2Y4 heterodimer." Proc Natl Acad Sci U S A. 2001; 98(18): 10073-8. PubMed: 11526233
| Comments:
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Comments |
Yeast has two R2 subunits (RNR2 and RNR4 or Y2 and Y4). Y2 provides the iron-radical center and Y4 is required in the assembly of the diferric-Y cofactor in Y2. Refer to DP00487 for RNR2 or Y2.
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