| General information | | DisProt: | DP00488 | | Name: | Ribonucleoside-diphosphate reductase small chain 2 | | Synonym(s): | RIR4_YEAST
EC=1.17.4.1
Ribonucleotide reductase small subunit 2
Ribonucleotide reductase R2 subunit 2
| | First appeared in release: | Release 3.2 (05/26/2006) | | UniProt: | P49723 | | UniGene: | | | SwissProt: | RIR4_YEAST | | TrEMBL: | | | NCBI (GI): | 1710402 | | Source organism: | Saccharomyces cerevisiae (Baker's yeast) | | Sequence length: | 345 | | Percent disordered: | 23% | | Homologues: | |
| Native sequence |
10 20 30 40 50 60
| | | | | |
MEAHNQFLKT FQKERHDMKE AEKDEILLME NSRRFVMFPI KYHEIWAAYK KVEASFWTAE - 60
EIELAKDTED FQKLTDDQKT YIGNLLALSI SSDNLVNKYL IENFSAQLQN PEGKSFYGFQ - 120
IMMENIYSEV YSMMVDAFFK DPKNIPLFKE IANLPEVKHK AAFIERWISN DDSLYAERLV - 180
AFAAKEGIFQ AGNYASMFWL TDKKIMPGLA MANRNICRDR GAYTDFSCLL FAHLRTKPNP - 240
KIIEKIITEA VEIEKEYYSN SLPVEKFGMD LKSIHTYIEF VADGLLQGFG NEKYYNAVNP - 300
FEFMEDVATA GKTTFFEKKV SDYQKASDMS KSATPSKEIN FDDDF
|
| Functional narrative |
Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. RNR4 is required for proper folding of RNR2 and assembly with the large subunits.
|
| Map of ordered and disordered regions |
Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.
|
| Region 1 | | Type: | Disordered | | Name: | | | Location: | 1 - 10 | | Length: | 10 | | Region sequence: |
MEAHNQFLKT | | Modification type: | Complex
| | PDB: | | | Structural/functional type: | Relationship to function unknown
| | Functional classes: | Unknown
| | Functional subclasses: | Unknown
| Detection methods:
- X-ray crystallography (acetate buffer (pH 4.9) 100 mM; NaCl 200 mM; PEG 4000 (14% wt/vol))
| References:
- Voegtli WC, Ge J, Perlstein DL, Stubbe J, Rosenzweig AC. "Structure of the yeast ribonucleotide reductase Y2Y4 heterodimer." Proc Natl Acad Sci U S A. 2001; 98(18): 10073-8. PubMed: 11526233
| Comments:
|
| Region 2 | | Type: | Disordered | | Name: | | | Location: | 91 - 99 | | Length: | 9 | | Region sequence: |
SSDNLVNKY | | Modification type: | Complex
| | PDB: | | | Structural/functional type: | Relationship to function unknown
| | Functional classes: | Unknown
| | Functional subclasses: | Unknown
| Detection methods:
- X-ray crystallography (acetate buffer (pH 4.9) 100 mM; NaCl 200 mM; PEG 4000 (14% wt/vol))
| References:
- Voegtli WC, Ge J, Perlstein DL, Stubbe J, Rosenzweig AC. "Structure of the yeast ribonucleotide reductase Y2Y4 heterodimer." Proc Natl Acad Sci U S A. 2001; 98(18): 10073-8. PubMed: 11526233
| Comments:
|
| Region 3 | | Type: | Disordered | | Name: | | | Location: | 264 - 274 | | Length: | 11 | | Region sequence: |
VEKFGMDLKSI | | Modification type: | Complex
| | PDB: | | | Structural/functional type: | Relationship to function unknown
| | Functional classes: | Unknown
| | Functional subclasses: | Unknown
| Detection methods:
- X-ray crystallography (acetate buffer (pH 4.9) 100 mM; NaCl 200 mM; PEG 4000 (14% wt/vol))
| References:
- Voegtli WC, Ge J, Perlstein DL, Stubbe J, Rosenzweig AC. "Structure of the yeast ribonucleotide reductase Y2Y4 heterodimer." Proc Natl Acad Sci U S A. 2001; 98(18): 10073-8. PubMed: 11526233
| Comments:
|
| Region 4 | | Type: | Disordered | | Name: | | | Location: | 295 - 345 | | Length: | 51 | | Region sequence: |
YNAVNPFEFMEDVATAGKTTFFEKKVSDYQKASDMSKSATPSKEINFDDDF | | Modification type: | Complex
| | PDB: | | | Structural/functional type: | Relationship to function unknown
| | Functional classes: | Unknown
| | Functional subclasses: | Protein-protein binding
| Detection methods:
- X-ray crystallography (acetate buffer (pH 4.9) 100 mM; NaCl 200 mM; PEG 4000 (14% wt/vol))
| References:
- Voegtli WC, Ge J, Perlstein DL, Stubbe J, Rosenzweig AC. "Structure of the yeast ribonucleotide reductase Y2Y4 heterodimer." Proc Natl Acad Sci U S A. 2001; 98(18): 10073-8. PubMed: 11526233
| Comments:
|
| Comments |
Yeast has two R2 subunits (RNR2 and RNR4 or Y2 and Y4). Y2 provides the iron-radical center and Y4 is required in the assembly of the diferric-Y cofactor in Y2. Refer to DP00487 for RNR2 or Y2.
|
| If you have any comments or wish to provide additional references to this protein or its disordered region(s), please click here to e-mail us. |
Disprot-footer
|
