| General information | | DisProt: | DP00611 | | Name: | Wee1-like protein kinase | | Synonym(s): | WEE1_HUMAN
Wee1A kinase
WEE1hu
Wee1
EC=2.7.10.2
| | First appeared in release: | Release 5.2 (08/07/2010) | | UniProt: | P30291 | | UniGene: | Hs.249441 | | SwissProt: | WEE1_HUMAN | | TrEMBL: | | | NCBI (GI): | 1351419 | | Source organism: | Homo sapiens (Human) | | Sequence length: | 646 | | Percent disordered: | 45% | | Homologues: | |
| Native sequence |
10 20 30 40 50 60
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MSFLSRQQPP PPRRAGAACT LRQKLIFSPC SDCEEEEEEE EEEGSGHSTG EDSAFQEPDS - 60
PLPPARSPTE PGPERRRSPG PAPGSPGELE EDLLLPGACP GADEAGGGAE GDSWEEEGFG - 120
SSSPVKSPAA PYFLGSSFSP VRCGGPGDAS PRGCGARRAG EGRRSPRPDH PGTPPHKTFR - 180
KLRLFDTPHT PKSLLSKARG IDSSSVKLRG SSLFMDTEKS GKREFDVRQT PQVNINPFTP - 240
DSLLLHSSGQ CRRRKRTYWN DSCGEDMEAS DYELEDETRP AKRITITESN MKSRYTTEFH - 300
ELEKIGSGEF GSVFKCVKRL DGCIYAIKRS KKPLAGSVDE QNALREVYAH AVLGQHSHVV - 360
RYFSAWAEDD HMLIQNEYCN GGSLADAISE NYRIMSYFKE AELKDLLLQV GRGLRYIHSM - 420
SLVHMDIKPS NIFISRTSIP NAASEEGDED DWASNKVMFK IGDLGHVTRI SSPQVEEGDS - 480
RFLANEVLQE NYTHLPKADI FALALTVVCA AGAEPLPRNG DQWHEIRQGR LPRIPQVLSQ - 540
EFTELLKVMI HPDPERRPSA MALVKHSVLL SASRKSAEQL RIELNAEKFK NSLLQKELKK - 600
AQMAKAAAEE RALFTDRMAT RSTTQSNRTS RLIGKKMNRS VSLTIY
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| Functional narrative |
May act as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDC2 before the onset of mitosis. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation. Specifically phosphorylates and inactivates cyclin B1-complexed CDC2 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDC2 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDC2 does not occur.
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| Map of ordered and disordered regions |
Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.
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| Region 1 | | Type: | Disordered | | Name: | RXL binding motif | | Location: | 1 - 292 | | Length: | 292 | | Region sequence: |
MSFLSRQQPPPPRRAGAACTLRQKLIFSPCSDCEEEEEEEEEEGSGHSTGEDSAFQEPDS
PLPPARSPTEPGPERRRSPGPAPGSPGELEEDLLLPGACPGADEAGGGAEGDSWEEEGFG
SSSPVKSPAAPYFLGSSFSPVRCGGPGDASPRGCGARRAGEGRRSPRPDHPGTPPHKTFR
KLRLFDTPHTPKSLLSKARGIDSSSVKLRGSSLFMDTEKSGKREFDVRQTPQVNINPFTP
DSLLLHSSGQCRRRKRTYWNDSCGEDMEASDYELEDETRPAKRITITESNMK | | Modification type: | Native
| | PDB: | 1X8B:A | | Structural/functional type: | | | Functional classes: | Modification site
Molecular recognition effectors
| | Functional subclasses: | Nuclear localization
Phosphorylation
Protein inhibitor
| Detection methods:
- Immunochemistry
| References:
- Li C, Andrake M, Dunbrack R, Enders GH. "A bifunctional regulatory element in human somatic wee1 mediates cyclin a/cdk2 binding and crm1-dependent nuclear export." Mol Cell Biol. ; 30(1): 116-30. PubMed: 19858290
| Comments:
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| References |
- Li C, Andrake M, Dunbrack R, Enders GH. "A bifunctional regulatory element in human somatic wee1 mediates cyclin a/cdk2 binding and crm1-dependent nuclear export." Mol Cell Biol. ; 30(1): 116-30. PubMed: 19858290
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| Comments |
AV (7-7-2010) PubMed: 15837193
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