| General information | | DisProt: | DP00624 | | Name: | DNA damage-inducible transcript 3 | | Synonym(s): | DDIT3_HUMAN
DDIT-3
Growth arrest and DNA-damage-inducible protein GADD153
C/EBP-homologous protein
CHOP
| | First appeared in release: | Release 5.6 (01/10/2011) | | UniProt: | P35638 | | UniGene: | Hs.505777 | | SwissProt: | DDIT3_HUMAN | | TrEMBL: | | | NCBI (GI): | 544022 | | Source organism: | Homo sapiens (Human) | | Sequence length: | 169 | | Percent disordered: | 31% | | Homologues: | |
| Native sequence |
10 20 30 40 50 60
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MAAESLPFSF GTLSSWELEA WYEDLQEVLS SDENGGTYVS PPGNEEEESK IFTTLDPASL - 60
AWLTEEEPEP AEVTSTSQSP HSPDSSQSSL AQEEEEEDQG RTRKRKQSGH SPARAGKQRM - 120
KEKEQENERK VAQLAEENER LKQEIERLTR EVEATRRALI DRMVNLHQA
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| Functional narrative |
As a transcription factor, CHOP's C-terminal domain binds to DNA. The N-termainal domain plays a regulatory role and is hence termed the transactivation domain. Inhibits the DNA-binding activity of C/EBP and LAP by forming heterodimers that cannot bind DNA. In the absence of the N-terminal region of CHOP, the ability to form oligomers is hindered.A secondary function of CHOP may be in the inhibition of Wnt/Tcf signaling and stimulation of c-Jun and sucrase-isomaltase reporter activity in intestinal colon cancer cells; the latter is dependent upon the N-terminus of CHOP and its oligomerization state.
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| Map of ordered and disordered regions |
Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.
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| Region 1 | | Type: | Disordered | | Name: | coiled coil | | Location: | 116 - 168 | | Length: | 53 | | Region sequence: |
GKQRMKEKEQENERKVAQLAEENERLKQEIERLTREVEATRRALIDRMVNLHQ | | Modification type: | Native
| | PDB: | | | Structural/functional type: | Function arises via a disorder to order transition
| | Functional classes: | Molecular recognition effectors
| | Functional subclasses: | Transactivation (transcriptional activation)
| Detection methods:
- Circular dichroism (CD) spectroscopy, near-UV (298 K; )
- Nuclear magnetic resonance (NMR) (277 K; )
- Size exclusion/gel filtration chromatography
| References:
- Singh VK, Pacheco I, Uversky VN, Smith SP, MacLeod RJ, Jia Z. "Intrinsically disordered human C/EBP homologous protein regulates biological activity of colon cancer cells during calcium stress." J Mol Biol. 2008; 380(2): 313-26. PubMed: 18534616
| Comments:Disordered N-terminal domain regulates multi-functional biological activity and oligomerization state of CHOP; inhibits beta-Catenin activity and stimulates c-Jun and SI reporter activity in intestinal cells.
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| References |
- Singh VK, Pacheco I, Uversky VN, Smith SP, MacLeod RJ, Jia Z. "Intrinsically disordered human C/EBP homologous protein regulates biological activity of colon cancer cells during calcium stress." J Mol Biol. 2008; 380(2): 313-26. PubMed: 18534616
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| Comments |
Additional UniGene ID: Hs.690217
Author verified 12-5-2010 (PubMed: 18534616)
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