| General information | | DisProt: | DP00631 | | Name: | Protein SIC1 | | Synonym(s): | SIC1_YEAST
CDK inhibitor p40
| | First appeared in release: | Release 5.2 (08/07/2010) | | UniProt: | P38634 | | UniGene: | | | SwissProt: | SIC1_YEAST | | TrEMBL: | | | NCBI (GI): | 585990 | | Source organism: | Saccharomyces cerevisiae (Baker's yeast) | | Sequence length: | 284 | | Percent disordered: | 32% | | Homologues: | |
| Native sequence |
10 20 30 40 50 60
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MTPSTPPRSR GTRYLAQPSG NTSSSALMQG QKTPQKPSQN LVPVTPSTTK SFKNAPLLAP - 60
PNSNMGMTSP FNGLTSPQRS PFPKSSVKRT LFQFESHDNG TVREEQEPLG RVNRILFPTQ - 120
QNVDIDAAEE EEEGEVLLPP SRPTSARQLH LSLERDEFDQ THRKKIIKDV PGTPSDKVIT - 180
FELAKNWNNN SPKNDARSQE SEDEEDIIIN PVRVGKNPFA SDELVTQEIR NERKRAMLRE - 240
NPDIEDVITY VNKKGEVVEK RRLTDEEKRR FKPKALFQSR DQEH
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| Functional narrative |
Substrate and inhibitor of the cyclin-dependent protein kinase CDC28. Its activity could be important for faithful segregation of chromosomes to daughter cells. It acts in response to a signal from a post-start checkpoint.
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| Map of ordered and disordered regions |
Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.
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| Region 1 | | Type: | Disordered | | Name: | N-terminal domain (NTD) | | Location: | 1 - 90 | | Length: | 90 | | Region sequence: |
MTPSTPPRSRGTRYLAQPSGNTSSSALMQGQKTPQKPSQNLVPVTPSTTKSFKNAPLLAP
PNSNMGMTSPFNGLTSPQRSPFPKSSVKRT | | Modification type: | Engineered
Fragment
| | PDB: | | | Structural/functional type: | Relationship to function unknown
| | Functional classes: | Molecular recognition effectors
| | Functional subclasses: | Protein-protein binding
| Detection methods:
- Circular dichroism (CD) spectroscopy, far-UV
| References:
- Borg M, Mittag T, Pawson T, Tyers M, Forman-Kay JD, Chan HS. "Polyelectrostatic interactions of disordered ligands suggest a physical basis for ultrasensitivity." Proc Natl Acad Sci U S A. 2007; 104(23): 9650-5. PubMed: 17522259
- Mittag T, Marsh J, Grishaev A, Orlicky S, Lin H, Sicheri F, Tyers M, Forman-Kay JD. "Structure/function implications in a dynamic complex of the intrinsically disordered Sic1 with the Cdc4 subunit of an SCF ubiquitin ligase." Structure. 2010; 18(4): 494-506. PubMed: 20399178
- Mittag T, Orlicky S, Choy WY, Tang X, Lin H, Sicheri F, Kay LE, Tyers M, Forman-Kay JD. "Dynamic equilibrium engagement of a polyvalent ligand with a single-site receptor." Proc. Natl. Acad. Sci. U.S.A.. 2008; 105(46): 17772-7. PubMed: 17660831
- Nash P, Tang X, Orlicky S, Chen Q, Gertler FB, Mendenhall MD, Sicheri F, Pawson T, Tyers M. "Multisite phosphorylation of a CDK inhibitor sets a threshold for the onset of DNA replication." Nature. 2001; 414(6863): 514-21. PubMed: 11734834
| Comments:Nash, et al. (2001) determined a lack of secondary structure in Sic1 by circular dichroism, as well as the necessity of multi-site phosphorylation of Sic1 for DNA replication to proceed.
Mittag, et al. (2008, 2010) explore dynamics of structure/function interplay of the multi-site phosphorylation requirement.
Borg, et al. (2007) explore electrostatic interactions as the mechanism behind the need for multi-site phosphorylation.
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| References |
- Borg M, Mittag T, Pawson T, Tyers M, Forman-Kay JD, Chan HS. "Polyelectrostatic interactions of disordered ligands suggest a physical basis for ultrasensitivity." Proc Natl Acad Sci U S A. 2007; 104(23): 9650-5. PubMed: 17522259
- Mittag T, Marsh J, Grishaev A, Orlicky S, Lin H, Sicheri F, Tyers M, Forman-Kay JD. "Structure/function implications in a dynamic complex of the intrinsically disordered Sic1 with the Cdc4 subunit of an SCF ubiquitin ligase." Structure. 2010; 18(4): 494-506. PubMed: 20399178
- Mittag T, Orlicky S, Choy WY, Tang X, Lin H, Sicheri F, Kay LE, Tyers M, Forman-Kay JD. "Dynamic equilibrium engagement of a polyvalent ligand with a single-site receptor." Proc. Natl. Acad. Sci. U.S.A.. 2008; 105(46): 17772-7. PubMed: 17660831
- Nash P, Tang X, Orlicky S, Chen Q, Gertler FB, Mendenhall MD, Sicheri F, Pawson T, Tyers M. "Multisite phosphorylation of a CDK inhibitor sets a threshold for the onset of DNA replication." Nature. 2001; 414(6863): 514-21. PubMed: 11734834
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| Comments |
AV (7-28-2010) PMID: 19008353
approved by Julie Forman-Kay on 7-29-2010
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