| General information | | DisProt: | DP00640 | | Name: | Nucleoprotein | | Synonym(s): | NCAP_MEASF
Nucleoprotein
Nucleocapsid protein
NP
Protein N
| | First appeared in release: | Release 5.5 (11/17/2010) | | UniProt: | Q89933 | | UniGene: | | | SwissProt: | NCAP_MEASF | | TrEMBL: | | | NCBI (GI): | 81945563 | | Source organism: | Measles virus (strain Edmonston B) (MeV) (Subacute sclerose panencephalitis virus) | | Sequence length: | 525 | | Percent disordered: | 24% | | Homologues: | |
| Native sequence |
10 20 30 40 50 60
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MATLLRSLAL FKRNKDKPPI TSGSGGAIRG IKHIIIVPIP GDSSITTRSR LLDRLVRLIG - 60
NPDVSGPKLT GALIGILSLF VESPGQLIQR ITDDPDVSIR LLEVVQSDQS QSGLTFASRG - 120
TNMEDEADQY FSHDDPISSD QSRFGWFENK EISDIEVQDP EGFNMILGTI LAQIWVLLAK - 180
AVTAPDTAAD SELRRWIKYT QQRRVVGEFR LERKWLDVVR NRIAEDLSLR RFMVALILDI - 240
KRTPGNKPRI AEMICDIDTY IVEAGLASFI LTIKFGIETM YPALGLHEFA GELSTLESLM - 300
NLYQQMGETA PYMVILENSI QNKFSAGSYP LLWSYAMGVG VELENSMGGL NFGRSYFDPA - 360
YFRLGQEMVR RSAGKVSSTL ASELGITAED ARLVSEIAMH TTEDKISRAV GPRQAQVSFL - 420
HGDQSENELP RLGGKEDRRV KQSRGEARES YRETGPSRAS DARAAHLPTG TPLDIDTASE - 480
SSQDPQDSRR SADALLRLQA MAGISEEQGS DTDTPIVYND RNLLD
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| Functional narrative |
Encapsidates the genome in a ratio of 1 N per 6 ribonucleotides, protecting it from nucleases. The nucleocapsid (NC) has an helical structure with either 12.35 or 11.64 N per turn, approximately 20nm in diameter, with a hollow central cavity approximately 5nm in diameter. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. During replication, encapsidation by N is coupled to RNA synthesis and all replicative products are resistant to nucleases. N is released in the blood following lysis of measles infected cells, it interacts then with human FCGR2B on immune cells, inducing apoptosis and blocking inflammatory immune response. Ntail binds to a protein on human thymic epithelial cells, termed Nucleoprotein Receptor (NR), inducing growth arrest.
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| Map of ordered and disordered regions |
Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.
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| Region 1 | | Type: | Disordered | | Name: | N TAIL | | Location: | 401 - 525 | | Length: | 125 | | Region sequence: |
TTEDKISRAVGPRQAQVSFLHGDQSENELPRLGGKEDRRVKQSRGEARESYRETGPSRAS
DARAAHLPTGTPLDIDTASESSQDPQDSRRSADALLRLQAMAGISEEQGSDTDTPIVYND
RNLLD | | Modification type: | Natural variant
| | PDB: | | | Structural/functional type: | | | Functional classes: | | | Functional subclasses: | Protein-genomic RNA binding
Protein-protein binding
| Detection methods:
- Sensitivity to proteolysis (310 K; pH: 7.5; DTT 0.5 mM; Laemmli (sample buffer) 2 X; NaCl 50 mM; N protein (sample) 1 mg/mL; PMSF 1 Mm; sodium phosphate buffer 50 mM; trypsin (protease))
- Circular dichroism (CD) spectroscopy, far-UV (293 K; pH: 7; EDTA 0.5 mM; Jasco 800 (dichrograph); PMSF 0.5 mM; quartz cells 1 mm; sodum phosphate 10 mM)
| References:
- Karlin D, Longhi S, Canard B. "Substitution of two residues in the measles virus nucleoprotein results in an impaired self-association." Virology. 2002; 302(2): 420-432. PubMed: 12441086
| Comments:
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| References |
- Karlin D, Ferron F, Canard B, Longhi S. "Structural disorder and modular organization in Paramyxovirinae N and P." 2003; 84(Pt 12): 3239-52. PubMed: 14645906
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