| General information | | DisProt: | DP00645 | | Name: | Bcl-2-modifying factor | | Synonym(s): | BMF_MOUSE
| | First appeared in release: | Release 5.1 (05/28/2010) | | UniProt: | Q91ZE9 | | UniGene: | Mm.210125 | | SwissProt: | BMF_MOUSE | | TrEMBL: | | | NCBI (GI): | 56748562 | | Source organism: | Mus musculus (Mouse) | | Sequence length: | 185 | | Percent disordered: | 86% | | Homologues: | |
| Native sequence |
10 20 30 40 50 60
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MEPPQCVEEL EDDVFQSEDG EPGTQPGGLL SADLFAQSQL DCPLSRLQLF PLTHCCGPGL - 60
RPISQEDKAT QTLSPASPSQ GVMLPCGVTE EPQRLFYGNA GYRLPLPASF PAGSPLGEQP - 120
PEGQFLQHRA EVQIARKLQC IADQFHRLHT QQHQQNRDRA WWQVFLFLQN LALNRQENRE - 180
GVGPW
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| Functional narrative |
May play a role in apoptosis.
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| Map of ordered and disordered regions |
Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.
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| Region 1 | | Type: | Disordered | | Name: | Bmf-C25 | | Location: | 1 - 160 | | Length: | 160 | | Region sequence: |
MEPPQCVEELEDDVFQSEDGEPGTQPGGLLSADLFAQSQLDCPLSRLQLFPLTHCCGPGL
RPISQEDKATQTLSPASPSQGVMLPCGVTEEPQRLFYGNAGYRLPLPASFPAGSPLGEQP
PEGQFLQHRAEVQIARKLQCIADQFHRLHTQQHQQNRDRA | | Modification type: | Fragment
| | PDB: | | | Structural/functional type: | | | Functional classes: | | | Functional subclasses: | Protein-protein binding
| Detection methods:
- Circular dichroism (CD) spectroscopy, far-UV (pH: 6.7; Olis (spectro-polarimeter ); protein concentration range (10-50 uM); sodium phosphate (buffer) 5 mM; resolution 1 nm; integration time 3 s; pathlength cuvettes (1-2 mm))
- Analytical ultracentrifugation (293 K; pH: 7.4; Optima XL-A (analytical ultracentrifuge); phosphate-buffered saline (buffer) 400 uM; protein (sample) 0.8 mg/mL; speed 50000 rpm)
- Size exclusion/gel filtration chromatography (pH: 7.4; fraction 500 uL; PBS; Superdex S200 column 20 mL)
- Nuclear magnetic resonance (NMR) (298 K; pH: 6.7; Bruker DRX-600 (spectrometer); NaCl 70 mM; protein (sample); sodium azide (in H2O:2H2O (95:5)) 0.04 %; sodium phosphate (buffer) 0.5 mM; TCEP 20 mM)
| References:
- Hinds MG, Smits C, Fredericks-Short R, Risk JM, Bailey M, Huang DC, Day CL. "Bim, Bad and Bmf: intrinsically unstructured BH3-only proteins that undergo a localized conformational change upon binding to prosurvival Bcl-2 targets." Cell Death Differ. 2006. PubMed: 16645638
| Comments:
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| References |
- Hinds MG, Smits C, Fredericks-Short R, Risk JM, Bailey M, Huang DC, Day CL. "Bim, Bad and Bmf: intrinsically unstructured BH3-only proteins that undergo a localized conformational change upon binding to prosurvival Bcl-2 targets." Cell Death Differ. 2006. PubMed: 16645638
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