DP00659: Telomere-binding protein subunit betaFASTA viewXML view

General information
DisProt:DP00659
Name:Telomere-binding protein subunit beta
Synonym(s):TEBB_OXYNO
Telomere-binding protein 41 kDa subunit
TEBP beta
First appeared in release:Release 5.2 (08/07/2010)
UniProt:P16458
UniGene: 
SwissProt: TEBB_OXYNO
TrEMBL:  
NCBI (GI): 135390
Source organism:Oxytricha nova
Sequence length:385
Percent disordered:40%
Homologues: 


Native sequence

        10         20         30         40         50         60
         |          |          |          |          |          |
MSKGASAPQQ QSAFKQLYTE LFNNEGDFSK VSSNLKKPLK CYVKESYPHF LVTDGYFFVA - 60
PYFTKEAVNE FHAKFPNVNI VDLTDKVIVI NNWSLELRRV NSAEVFTSYA NLEARLIVHS - 120
FKPNLQERLN PTRYPVNLFR DDEFKTTIQH FRHTALQAAI NKTVKGDNLV DISKVADAAG - 180
KKGKVDAGIV KASASKGDEF SDFSFKEGNT ATLKIADIFV QEKGKDALNK AADHTDGAKV - 240
KGGAKGKGKA AAKAAKGKKL SAKKGDSSAA DVRKSVDKIV KYTPSKGSRK DTPQKSQAPA - 300
AGKSSAKKGG KKAVPSAPSP SGKKSALTTD KMTMAQFVKY LDWHEKKKGG KVSSGGKVLG - 360
KRSAGKASAT SGKASKASKK TAAKK



Functional narrative    

May function as protective capping of the single-stranded telomeric overhang. May also participate in telomere length regulation during DNA replication. Bind specifically to the T4G4-containing extension on the 3'strand and protect this region of the telomere from nuclease digestion and chemical modification.

Region 1: 233-385

Map of ordered and disordered regions







Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.


Region 1
Type:Disordered
Name:C-terminal domain
Location:233 - 385
Length:153
Region sequence:

DHTDGAKVKGGAKGKGKAAAKAAKGKKLSAKKGDSSAADVRKSVDKIVKYTPSKGSRKDT
PQKSQAPAAGKSSAKKGGKKAVPSAPSPSGKKSALTTDKMTMAQFVKYLDWHEKKKGGKV
SSGGKVLGKRSAGKASATSGKASKASKKTAAKK

Modification type: Native
PDB:  
Structural/functional type:  
Functional classes:  
Functional subclasses: Protein-DNA binding
Detection methods:
  1. Raman spectroscopy (pH: 7.5; 1877 Triplemate (spectrograph); EDTA (buffer) 0.5 mM; excitation wavelength 514.5 nm; glycerol; NaCl (buffer) 50 mM; protein subunit (sample) 50 mg/mL; radiant power 200 mW; Tris (buffer) 5 mM)

References:
  1. Buczek P, Horvath MP. "Structural reorganization and the cooperative binding of single-stranded telomere DNA in Sterkiella nova." J Biol Chem. 2006; 281(52): 40124-34. PubMed: 17082188

  2. Laporte L, Stultz J, Thomas GJ Jr. "Solution conformations and interactions of alpha and beta subunits of the Oxytricha nova telomere binding protein: investigation by Raman spectroscopy." Biochemistry. 1997; 36(26): 8053-8059. PubMed: 9201953

Comments:
 



References

  1. Buczek P, Horvath MP. "Structural reorganization and the cooperative binding of single-stranded telomere DNA in Sterkiella nova." J Biol Chem. 2006; 281(52): 40124-34. PubMed: 17082188



Comments


AV (8-2-2010) PubMed: 17082188


If you have any comments or wish to provide additional references to this protein or its disordered region(s), please click here to e-mail us.


Disprot-footer
Contact us