| General information | | DisProt: | DP00659 | | Name: | Telomere-binding protein subunit beta | | Synonym(s): | TEBB_OXYNO
Telomere-binding protein 41 kDa subunit
TEBP beta
| | First appeared in release: | Release 5.2 (08/07/2010) | | UniProt: | P16458 | | UniGene: | | | SwissProt: | TEBB_OXYNO | | TrEMBL: | | | NCBI (GI): | 135390 | | Source organism: | Oxytricha nova | | Sequence length: | 385 | | Percent disordered: | 40% | | Homologues: | |
| Native sequence |
10 20 30 40 50 60
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MSKGASAPQQ QSAFKQLYTE LFNNEGDFSK VSSNLKKPLK CYVKESYPHF LVTDGYFFVA - 60
PYFTKEAVNE FHAKFPNVNI VDLTDKVIVI NNWSLELRRV NSAEVFTSYA NLEARLIVHS - 120
FKPNLQERLN PTRYPVNLFR DDEFKTTIQH FRHTALQAAI NKTVKGDNLV DISKVADAAG - 180
KKGKVDAGIV KASASKGDEF SDFSFKEGNT ATLKIADIFV QEKGKDALNK AADHTDGAKV - 240
KGGAKGKGKA AAKAAKGKKL SAKKGDSSAA DVRKSVDKIV KYTPSKGSRK DTPQKSQAPA - 300
AGKSSAKKGG KKAVPSAPSP SGKKSALTTD KMTMAQFVKY LDWHEKKKGG KVSSGGKVLG - 360
KRSAGKASAT SGKASKASKK TAAKK
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| Functional narrative |
May function as protective capping of the single-stranded telomeric overhang. May also participate in telomere length regulation during DNA replication. Bind specifically to the T4G4-containing extension on the 3'strand and protect this region of the telomere from nuclease digestion and chemical modification.
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| Map of ordered and disordered regions |
Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.
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| Region 1 | | Type: | Disordered | | Name: | C-terminal domain | | Location: | 233 - 385 | | Length: | 153 | | Region sequence: |
DHTDGAKVKGGAKGKGKAAAKAAKGKKLSAKKGDSSAADVRKSVDKIVKYTPSKGSRKDT
PQKSQAPAAGKSSAKKGGKKAVPSAPSPSGKKSALTTDKMTMAQFVKYLDWHEKKKGGKV
SSGGKVLGKRSAGKASATSGKASKASKKTAAKK | | Modification type: | Native
| | PDB: | | | Structural/functional type: | | | Functional classes: | | | Functional subclasses: | Protein-DNA binding
| Detection methods:
- Raman spectroscopy (pH: 7.5; 1877 Triplemate (spectrograph); EDTA (buffer) 0.5 mM; excitation wavelength 514.5 nm; glycerol; NaCl (buffer) 50 mM; protein subunit (sample) 50 mg/mL; radiant power 200 mW; Tris (buffer) 5 mM)
| References:
- Buczek P, Horvath MP. "Structural reorganization and the cooperative binding of single-stranded telomere DNA in Sterkiella nova." J Biol Chem. 2006; 281(52): 40124-34. PubMed: 17082188
- Laporte L, Stultz J, Thomas GJ Jr. "Solution conformations and interactions of alpha and beta subunits of the Oxytricha nova telomere binding protein: investigation by Raman spectroscopy." Biochemistry. 1997; 36(26): 8053-8059. PubMed: 9201953
| Comments:
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| References |
- Buczek P, Horvath MP. "Structural reorganization and the cooperative binding of single-stranded telomere DNA in Sterkiella nova." J Biol Chem. 2006; 281(52): 40124-34. PubMed: 17082188
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| Comments |
AV (8-2-2010) PubMed: 17082188
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