DP00664: 18 kDa seed maturation proteinFASTA viewXML view

General information
DisProt:DP00664
Name:18 kDa seed maturation protein
Synonym(s):PM1_SOYBN
GmPM1
18 kD late embryogenesis abundant protein
First appeared in release:Release 5.1 (05/28/2010)
UniProt:Q01417
UniGene:Gma.38
SwissProt: PM1_SOYBN
TrEMBL:  
NCBI (GI): 417494
Source organism:Glycine max (Soybean)
Sequence length:173
Percent disordered:100%
Homologues: 


Native sequence

        10         20         30         40         50         60
         |          |          |          |          |          |
MQGGKKAGES IKETATNIGA SAKAGMEKTK ATVQEKAERM TARDPVQKEL ATQKKEAKMN - 60
QAELDKQAAR QHNTAAKQSA TTAGHMGHGH HTTGTGTGTA TYSTTGEYGQ PMGAHQTSAM - 120
PGHGTGQPTG HVTEGVVGSH PIGTNRGPGG TATAHNTRAG GKPNDYGYGT GGT



Functional narrative    

18 kDa seed maturation protein is one of the Late Embryogenesis-abundant Group IV (LEA IV) proteins. Function and mechanisms are not currently known, but they may play a protective role for membranes and proteins sensitive to dehydration. LEA IV proteins are also an important component of cellular glasses.

Region 1: 1-173

Map of ordered and disordered regions







Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.


Region 1
Type:Disordered - Molten Globule
Name: 
Location:1 - 173
Length:173
Region sequence:

MQGGKKAGESIKETATNIGASAKAGMEKTKATVQEKAERMTARDPVQKELATQKKEAKMN
QAELDKQAARQHNTAAKQSATTAGHMGHGHHTTGTGTGTATYSTTGEYGQPMGAHQTSAM
PGHGTGQPTGHVTEGVVGSHPIGTNRGPGGTATAHNTRAGGKPNDYGYGTGGT

Modification type: Native
PDB:  
Structural/functional type: Function arises via a molten globule to order transition
Functional classes: Chaperones
Functional subclasses: Unknown
Detection methods:
  1. Circular dichroism (CD) spectroscopy, far-UV (283 K; pH: 7; Potassium phosphate (buffer) 10 mM; Recombinant protein (soln) 10 uM; SDS (4%); Temp range 283K to 363K; TFE (50%))

  2. Fourier transform infrared spectroscopy (FTIR), aka infrared spectroscopy) (Aqueous lyophilized protein in D2O; Dry protein/sugar/poly-L-lysine/phospholipid (films for air-drying, 8 uL droplet of 20 ug/uL in H2O); Fast air-drying (RT, 3% RH, 3h); Slow air-drying (RT, 65% RH 16h + 3% RH, 3h); Temp range 233K to 423K)

References:
  1. Shih MD, Hsieh TY, Lin TP, Hsing YI, Hoekstra FA. "Characterization of two soybean (Glycine max L.) LEA IV proteins by circular dichroism and Fourier transform infrared spectrometry." Plant Cell Physiol. . PubMed: 20071374

Comments:
 



References

  1. Shih MD, Hsieh TY, Lin TP, Hsing YI, Hoekstra FA. "Characterization of two soybean (Glycine max L.) LEA IV proteins by circular dichroism and Fourier transform infrared spectrometry." Plant Cell Physiol. . PubMed: 20071374


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