| General information | | DisProt: | DP00669 | | Name: | Seminal plasma protein BSP-30 kDa | | Synonym(s): | SFP4_BOVIN
BSP-30K
BSP5
Binder of Sperm 5 (BSP5)
| | First appeared in release: | Release 5.3 (09/21/2010) | | UniProt: | P81019 | | UniGene: | | | SwissProt: | SFP4_BOVIN | | TrEMBL: | | | NCBI (GI): | | | Source organism: | Bos taurus (Bovine) | | Sequence length: | 183 | | Percent disordered: | 31% | | Homologues: | |
| Native sequence |
10 20 30 40 50 60
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MAPLVGLFLI WAGASVFQQL HPVNGGDIPD PGSKPTPPGM ADELPTETYD LPPEIYTTTF - 60
LPRTIYPQEE MPYDDKPFPS LLSKANDLNA VFEGPACAFP FTYKGKKYYM CTRKNSVLLW - 120
CSLDTEYQGN WKFCTERDEP ECVFPFIYRK KSYESCTRVH SFFWRRWCSL TSNYDRDKAW - 180
KYC
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| Functional narrative |
The mature (secreted) peptide contains 158 amino acids. The signal peptide is 25 amino acids long. With the signal peptide, its length extends to 183 amino acids. Binds to spermatozoa upon ejaculation and may play a role in sperm capacitation. Displays heparin-, gelatin-, glycosaminoglycan-, phospholipid-, and lipoprotein-binding activities. Secreted. Belongs to the seminal plasma protein family, recently named as binder of sperm family. Contains 2 fibronectin type-II domains. (UniProt)
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| Map of ordered and disordered regions |
Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.
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| Region 1 | | Type: | Disordered - Extended | | Name: | N-terminal part | | Location: | 26 - 81 | | Length: | 56 | | Region sequence: |
GDIPDPGSKPTPPGMADELPTETYDLPPEIYTTTFLPRTIYPQEEMPYDDKPFPSL | | Modification type: | Fragment
Native
| | PDB: | | | Structural/functional type: | Relationship to function unknown
| | Functional classes: | Modification site
| | Functional subclasses: | Glycosylation
| Detection methods:
- Circular dichroism (CD) spectroscopy, far-UV (293 K; pH: 7.4; His-S-N-terminal fragment (protein) 0.25 mg/mL; sodium phosphate (buffer) 10 mM)
- Sensitivity to proteolysis
- SDS-PAGE gel, Aberrant mobility on
| References:
- Jois PS, Manjunath P. "The N-terminal part of Binder of SPerm 5 (BSP5), which promotes sperm capacitation in bovine species is intrinsically disordered." Biochem. Biophys. Res. Commun.. 2010; 394(4): 1036-41. PubMed: 20331968
- Salois D, Menard M, Paquette Y, Manjunath P. "Complementary deoxyribonucleic acid cloning and tissue expression of BSP-A3 and BSP-30-kDa: phosphatidylcholine and heparin-binding proteins of bovine seminal plasma." Biol. Reprod.. 1999; 61(1): 288-97. PubMed: 10377061
| Comments:
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| References |
- Jois PS, Manjunath P. "The N-terminal part of Binder of SPerm 5 (BSP5), which promotes sperm capacitation in bovine species is intrinsically disordered." Biochem. Biophys. Res. Commun.. 2010; 394(4): 1036-41. PubMed: 20331968
- Salois D, Menard M, Paquette Y, Manjunath P. "Complementary deoxyribonucleic acid cloning and tissue expression of BSP-A3 and BSP-30-kDa: phosphatidylcholine and heparin-binding proteins of bovine seminal plasma." Biol. Reprod.. 1999; 61(1): 288-97. PubMed: 10377061
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| Comments |
BSP1 and BSP3 are homologues.
The first 25 amino acids comprise the signal peptide.
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