DP00691: Regulator of chromosome condensationFASTA viewXML view

General information
DisProt:DP00691
Name:Regulator of chromosome condensation
Synonym(s):RCC1_HUMAN
RCC1
Chromosome condensation protein 1
Cell cycle regulatory protein
First appeared in release:Release 5.2 (08/07/2010)
UniProt:P18754
UniGene: 
SwissProt: RCC1_HUMAN
TrEMBL:  
NCBI (GI):  
Source organism:Homo sapiens (Human)
Sequence length:421
Percent disordered:12%
Homologues: 


Native sequence

        10         20         30         40         50         60
         |          |          |          |          |          |
MSPKRIAKRR SPPADAIPKS KKVKVSHRSH STEPGLVLTL GQGDVGQLGL GENVMERKKP - 60
ALVSIPEDVV QAEAGGMHTV CLSKSGQVYS FGCNDEGALG RDTSVEGSEM VPGKVELQEK - 120
VVQVSAGDSH TAALTDDGRV FLWGSFRDNN GVIGLLEPMK KSMVPVQVQL DVPVVKVASG - 180
NDHLVMLTAD GDLYTLGCGE QGQLGRVPEL FANRGGRQGL ERLLVPKCVM LKSRGSRGHV - 240
RFQDAFCGAY FTFAISHEGH VYGFGLSNYH QLGTPGTESC FIPQNLTSFK NSTKSWVGFS - 300
GGQHHTVCMD SEGKAYSLGR AEYGRLGLGE GAEEKSIPTL ISRLPAVSSV ACGASVGYAV - 360
TKDGRVFAWG MGTNYQLGTG QDEDAWSPVE MMGKQLENRV VLSVSSGGQH TVLLVKDKEQ - 420
S



Functional narrative    

Promotes the exchange of Ran-bound GDP by GTP. Involved in the regulation of onset of chromosome condensation in the S phase. Binds to the chromatin. RCC1/Ran complex (together with other proteins) acts as a component of a signal transmission pathway that detects unreplicated DNA. (UniProt). The binding of RCC1 (regulator of chromosome condensation 1) to chromatin is critical for cellular processes such as mitosis, nucleocytoplasmic transport, and nuclear envelope formation because RCC1 recruits the small GTPase Ran (Ras-related nuclear protein) to chromatin and sets up a Ran-GTP gradient around the chromosomes. (England, 2010)

Region 1: 2-20 Region 2: 198-223 Region 3: 415-421

Map of ordered and disordered regions







Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.


Region 1
Type:Disordered - Extended
Name:N-terminal domain
Location:2 - 20
Length:19
Region sequence:

SPKRIAKRRSPPADAIPKS

Modification type: Native
PDB: 1A12:A, 1I2M:D
Structural/functional type: Function arises from the disordered state
Functional classes: Molecular assembly
Molecular recognition effectors
Functional subclasses: Protein-DNA binding
Protein-protein binding
Detection methods:
  1. SDS-PAGE/Pull-down Assays (H50 buffer; hRCC1 protein 1 uM; nucleosome core particles (recombinant, STRHISN-tagged) 1 uM; Talon metal affinity resin 18 uL)
References:
  1. England JR, Huang J, Jennings MJ, Makde RD, Tan S. "RCC1 uses a conformationally diverse loop region to interact with the nucleosome: a model for the RCC1-nucleosome complex." J. Mol. Biol.. 2010; 398(4): 518-29. PubMed: 20347844
Comments:
RCC1 uses both the N-terminal tail and the switchback loop of the beta-propeller domain to bind the nucleosome. (England, 2010)


Region 2
Type:Disordered
Name:Swithcback loop
Location:198 - 223
Length:26
Region sequence:

CGEQGQLGRVPELFANRGGRQGLERL

Modification type: Fragment
Native
PDB: 1A12:A, 1I2M:D
Structural/functional type: Function arises from the disordered state
Functional classes: Molecular recognition effectors
Molecular assembly
Entropic chain
Functional subclasses: Protein-DNA binding
Protein-protein binding
Detection methods:
  1. SDS-PAGE/Pull-down Assays (pH: 7.4; GSH-Sepharose resin 20 uL; GST binding buffer; HisTrxN-LANA (binding competitor) 6.5 uM; hRCC1{delta}1 (GST-His tagged protein) 8 uM; recombinant nuclesome core particles (binding partner) 6.5 uM; T50Ac buffer)

  2. SDS-PAGE/Pull-down Assays (pH: 7.4; H50 buffer; hRCC1 protein 1 uM; nucleosome core particles (recombinant, STRHISN-tagged) 1 uM; Talon metal affinity resin 18 uL)
References:
  1. England JR, Huang J, Jennings MJ, Makde RD, Tan S. "RCC1 uses a conformationally diverse loop region to interact with the nucleosome: a model for the RCC1-nucleosome complex." J. Mol. Biol.. 2010; 398(4): 518-29. PubMed: 20347844
Comments:
RCC1 uses both the N-terminal tail and the switchback loop of the beta-propeller domain to bind the nucleosome. (England, 2010)

RCC1 switchback loop amino acids Arg 217 and Arg214 are critical for beta-propeller binding interactions with the nucleosome. (England 2010)


Region 3
Type:Disordered
Name:C-terminal
Location:415 - 421
Length:7
Region sequence:

VKDKEQS

Modification type: Native
PDB: 1A12:A
Structural/functional type: Function arises from the disordered state
Functional classes: Molecular assembly
Functional subclasses: Flexible linkers/spacers
Detection methods:
  1. X-ray crystallography (100 K; pH: 6.9; )
References:
  1. Renault L, Nassar N, Vetter I, Becker J, Klebe C, Roth M, Wittinghofer A. "The 1.7 A crystal structure of the regulator of chromosome condensation (RCC1) reveals a seven-bladed propeller." Nature. 1998; 392(6671): 97-101. PubMed: 9510255
Comments:
 



References

  1. England JR, Huang J, Jennings MJ, Makde RD, Tan S. "RCC1 uses a conformationally diverse loop region to interact with the nucleosome: a model for the RCC1-nucleosome complex." J. Mol. Biol.. 2010; 398(4): 518-29. PubMed: 20347844

  2. Renault L, Kuhlmann J, Henkel A, Wittinghofer A. "Structural basis for guanine nucleotide exchange on Ran by the regulator of chromosome condensation (RCC1)." Cell. 2001; 105(2): 245-55. PubMed: 11336674

  3. Renault L, Nassar N, Vetter I, Becker J, Klebe C, Roth M, Wittinghofer A. "The 1.7 A crystal structure of the regulator of chromosome condensation (RCC1) reveals a seven-bladed propeller." Nature. 1998; 392(6671): 97-101. PubMed: 9510255



Comments


AV (8-3-2010) PubMed: 20347844


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