General information | DisProt: | DP00699 | Name: | Phosphoprotein | Synonym(s): | PHOSP_NIPAV
Protein P
| First appeared in release: | Release 5.2 (08/07/2010) | UniProt: | Q9IK91 | UniGene: | | SwissProt: | PHOSP_NIPAV | TrEMBL: | | NCBI (GI): | | Source organism: | Nipah virus | Sequence length: | 709 | Percent disordered: | 57% | Homologues: | |
Native sequence |
10 20 30 40 50 60 | | | | | | MDKLELVNDG LNIIDFIQKN QKEIQKTYGR SSIQQPSIKD QTKAWEDFLQ CTSGESEQVE - 60 GGMSKDDGDV ERRNLEDLSS TSPTDGTIGK RVSNTRDWAE GSDDIQLDPV VTDVVYHDHG - 120 GECTGYGFTS SPERGWSDYT SGANNGNVCL VSDAKMLSYA PEIAVSKEDR ETDLVHLENK - 180 LSTTGLNPTA VPFTLRNLSD PAKDSPVIAE HYYGLGVKEQ NVGPQTSRNV NLDSIKLYTS - 240 DDEEADQLEF EDEFAGSSSE VIVGISPEDE EPSSVGGKPN ESIGRTIEGQ SIRDNLQAKD - 300 NKSTDVPGAG PKDSAVKEEP PQKRLPMLAE EFECSGSEDP IIRELLKENS LINCQQGKDA - 360 QPPYHWSIER SISPDKTEIV NGAVQTADRQ RPGTPMPKSR GIPIKKGTDA KYPSAGTENV - 420 PGSKSGATRH VRGSPPYQEG KSVNAENVQL NASTAVKETD KSEVNPVDDN DSLDDKYIMP - 480 SDDFSNTFFP HDTDRLNYHA DHLGDYDLET LCEESVLMGV INSIKLINLD MRLNHIEEQV - 540 KEIPKIINKL ESIDRVLAKT NTALSTIEGH LVSMMIMIPG KGKGERKGKN NPELKPVIGR - 600 DILEQQSLFS FDNVKNFRDG SLTNEPYGAA VQLREDLILP ELNFEETNAS QFVPMADDSS - 660 RDVIKTLIRT HIKDRELRSE LIGYLNKAEN DEEIQEIANT VNDIIDGNI
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Functional narrative |
Function: Essential component of the RNA polymerase transcription and replication complex. Binds the viral ribonucleocapsid and positions the L polymerase on the template (By similarity). May play a role to prevent the establishment of cellular antiviral state by binding to host STAT1 in the cytoplasm. This activity is not as strong as that of V and W.
SUBUNIT: Interacts with host STAT1.
Subcellular Location: Virion. Host cytoplasm.
RNA EDITING: Modified_positions=406; Note=Partially edited. RNA editing at this position consists of an insertion of one or two guanine nucleotides. The sequence displayed here is the P protein, derived from the unedited RNA. The edited RNA gives rise to the V protein (+1G) (AC Q997F2), and the W protein (+2G) (AC P0C1C7).
MISCELLANEOUS: The P/V/C gene has two overlapping open reading frames. One encodes the P/V/W proteins and the other the C protein. -----------------------------------------------------------------------
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Map of ordered and disordered regions |
Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.
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Region 1 | Type: | Disordered - Pre-Molten Globule | Name: | PNT | Location: | 1 - 406 | Length: | 406 | Region sequence: |
MDKLELVNDGLNIIDFIQKNQKEIQKTYGRSSIQQPSIKDQTKAWEDFLQCTSGESEQVE GGMSKDDGDVERRNLEDLSSTSPTDGTIGKRVSNTRDWAEGSDDIQLDPVVTDVVYHDHG GECTGYGFTSSPERGWSDYTSGANNGNVCLVSDAKMLSYAPEIAVSKEDRETDLVHLENK LSTTGLNPTAVPFTLRNLSDPAKDSPVIAEHYYGLGVKEQNVGPQTSRNVNLDSIKLYTS DDEEADQLEFEDEFAGSSSEVIVGISPEDEEPSSVGGKPNESIGRTIEGQSIRDNLQAKD NKSTDVPGAGPKDSAVKEEPPQKRLPMLAEEFECSGSEDPIIRELLKENSLINCQQGKDA QPPYHWSIERSISPDKTEIVNGAVQTADRQRPGTPMPKSRGIPIKK | Modification type: | Fragment
Monomeric
Native
| PDB: | | Structural/functional type: | Function arises via a pre-molten globule to order transition | Functional classes: | Molecular recognition effectors
| Functional subclasses: | Protein-protein binding
| Detection methods:
- Sensitivity to proteolysis (299 K; pH: 8; thermolysin)
- Nuclear magnetic resonance (NMR) (300 K; pH: 7; D2O 10 %; NaCl 150 mM; PNT 0.1 mM; sodium phosphate 10 mM)
- Circular dichroism (CD) spectroscopy, far-UV (293 K; pH: 7; )
- Dynamic light scattering (298 K; pH: 8; )
- SDS-PAGE gel, Aberrant mobility on
| References:
- Habchi J, Mamelli L, Darbon H, Longhi S. "Structural disorder within Henipavirus nucleoprotein and phosphoprotein: from predictions to experimental assessment." PLoS ONE. 2010; 5(7): e11684. PubMed: 20657787
| Comments:The PNT region is shared by Proteins P, V and W. According to Habchi et al. (2010), the disorder found in the PNT region of Protein P is "a way of alleviating evolutionary constraints within overlapping reading frames."
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References |
- Habchi J, Mamelli L, Darbon H, Longhi S. "Structural disorder within Henipavirus nucleoprotein and phosphoprotein: from predictions to experimental assessment." PLoS ONE. 2010; 5(7): e11684. PubMed: 20657787
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Comments |
[AV 08/06/2010 PMID 20657787]
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