Annotations for this protein have been verified by the authors of the corresponding papers



DP00700: PhosphoproteinFASTA viewXML view

General information
DisProt:DP00700
Name:Phosphoprotein
Synonym(s):PHOSP_HENDV
Protein P
First appeared in release:Release 5.2 (08/07/2010)
UniProt:O55778
UniGene: 
SwissProt: PHOSP_HENDV
TrEMBL:  
NCBI (GI):  
Source organism:Hendra virus
Sequence length:707
Percent disordered:57%
Homologues: 


Native sequence

        10         20         30         40         50         60
         |          |          |          |          |          |
MDKLDLVNDG LDIIDFIQKN QKEIQKTYGR SSIQQPSTKD RTRAWEDFLQ STSGEHEQAE - 60
GGMPKNDGGT EGRNVEDLSS VTSSDGTIGQ RVSNTRAWAE DPDDIQLDPM VTDVVYHDHG - 120
GECTGHGPSS SPERGWSYHM SGTHDGNVRA VPDTKVLPNA PKTTVPEEVR EIDLIGLEDK - 180
FASAGLNPAA VPFVPKNQST PTEEPPVIPE YYYGSGRRGD LSKSPPRGNV NLDSIKIYTS - 240
DDEDENQLEY EDEFAKSSSE VVIDTTPEDN DSINQEEVVG DPSDQGLEHP FPLGKFPEKE - 300
ETPDVRRKDS LMQDSCKRGG VPKRLPMLSE EFECSGSDDP IIQELEREGS HPGGSLRLRE - 360
PPQSSGNSRN QPDRQLKTGD AASPGGVQRP GTPMPKSRIM PIKKGTDAKS QYVGTEDVPG - 420
SKSGATRYVR GLPPNQESKS VTAENVQLSA PSAVTRNEGH DQEVTSNEDS LDDKYIMPSD - 480
DFANTFLPHD TDRLNYHADH LNDYDLETLC EESVLMGIVN AIKLINIDMR LNHIEEQMKE - 540
IPKIINKIDS IDRVLAKTNT ALSTIEGHLV SMMIMIPGKG KGERKGKTNP ELKPVIGRNI - 600
LEQQELFSFD NLKNFRDGSL TDEPYGGVAR IRDDLILPEL NFSETNASQF VPLADDASKD - 660
VVRTMIRTHI KDRELRSELM DYLNRAETDE EVQEVANTVN DIIDGNI



Functional narrative    

Function: Essential component of the RNA polymerase transcription and replication complex. Binds the viral ribonucleocapsid and positions the L polymerase on the template (By similarity).
RNA EDITING: Modified_positions=407; Note=Partially edited. RNA editing at this position consists of an insertion of one or two guanine nucleotides. The sequence displayed here is the P protein, derived from the unedited RNA. The edited RNA gives rise to the V protein (+1G) (AC O55777), and the W protein (+2G) (AC P0C1C6).
MISCELLANEOUS: The P/V/C gene has two overlapping open reading frames. One encodes the P/V/W proteins and the other the C protein.
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Region 1: 1-404

Map of ordered and disordered regions







Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.


Region 1
Type:Disordered - Pre-Molten Globule
Name:PNT
Location:1 - 404
Length:404
Region sequence:

MDKLDLVNDGLDIIDFIQKNQKEIQKTYGRSSIQQPSTKDRTRAWEDFLQSTSGEHEQAE
GGMPKNDGGTEGRNVEDLSSVTSSDGTIGQRVSNTRAWAEDPDDIQLDPMVTDVVYHDHG
GECTGHGPSSSPERGWSYHMSGTHDGNVRAVPDTKVLPNAPKTTVPEEVREIDLIGLEDK
FASAGLNPAAVPFVPKNQSTPTEEPPVIPEYYYGSGRRGDLSKSPPRGNVNLDSIKIYTS
DDEDENQLEYEDEFAKSSSEVVIDTTPEDNDSINQEEVVGDPSDQGLEHPFPLGKFPEKE
ETPDVRRKDSLMQDSCKRGGVPKRLPMLSEEFECSGSDDPIIQELEREGSHPGGSLRLRE
PPQSSGNSRNQPDRQLKTGDAASPGGVQRPGTPMPKSRIMPIKK

Modification type: Fragment
Monomeric
Native
PDB:  
Structural/functional type: Function arises via a pre-molten globule to order transition
Functional classes: Molecular recognition effectors
Functional subclasses: Protein-protein binding
Detection methods:
  1. Sensitivity to proteolysis (299 K; pH: 8; thermolysin)

  2. Nuclear magnetic resonance (NMR) (300 K; pH: 7; D2O 10 %; NaCl 150 mM; PNT 0.1 mM; sodium phosphate 10 mM)

  3. Circular dichroism (CD) spectroscopy, far-UV (293 K; pH: 7; )

  4. Dynamic light scattering (298 K; pH: 8; )

  5. SDS-PAGE gel, Aberrant mobility on

References:
  1. Habchi J, Mamelli L, Darbon H, Longhi S. "Structural disorder within Henipavirus nucleoprotein and phosphoprotein: from predictions to experimental assessment." PLoS ONE. 2010; 5(7): e11684. PubMed: 20657787

Comments:
The PNT region is shared by Proteins P, V and W. According to Habchi et al. (2010), the disorder found in the PNT region of Protein P is "a way of alleviating evolutionary constraints within overlapping reading frames."




References

  1. Habchi J, Mamelli L, Darbon H, Longhi S. "Structural disorder within Henipavirus nucleoprotein and phosphoprotein: from predictions to experimental assessment." PLoS ONE. 2010; 5(7): e11684. PubMed: 20657787



Comments


[AV 08/06/2010 PMID 20657787]


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