| General information | | DisProt: | DP00704 | | Name: | Growth arrest and DNA damage-inducible protein GADD45 alpha | | Synonym(s): | GA45A_HUMAN
DNA damage-inducible transcript 1 protein
DDIT-1
Gadd45-alpha
| | First appeared in release: | Release 5.6 (01/10/2011) | | UniProt: | P24522 | | UniGene: | | | SwissProt: | GA45A_HUMAN | | TrEMBL: | | | NCBI (GI): | | | Source organism: | Homo sapiens (Human) | | Sequence length: | 165 | | Percent disordered: | 25% | | Homologues: | |
| Native sequence |
10 20 30 40 50 60
| | | | | |
MTLEEFSAGE QKTERMDKVG DALEEVLSKA LSQRTITVGV YEAAKLLNVD PDNVVLCLLA - 60
ADEDDDRDVA LQIHFTLIQA FCCENDINIL RVSNPGRLAE LLLLETDAGP AASEGAEQPP - 120
DLHCVLVTNP HSSQWKDPAL SQLICFCRES RYMDQWVPVI NLPER
|
| Functional narrative |
Function: Binds to proliferating cell nuclear antigen. Might affect PCNA interaction with some CDK (cell division protein kinase) complexes; stimulates DNA excision repair in vitro and inhibits entry of cells into S phase.
SUBUNIT: Interacts with GADD45GIP1. Q8SPE7:GADD45GIP1 (xeno); NbExp=1; IntAct=EBI-448167, EBI-2267139; Q8TAE8:GADD45GIP1; NbExp=2; IntAct=EBI-448167, EBI-372506;
INDUCTION: By UV irradiation, X-rays, growth arrest and alkylating agents. The induction is mediate by some kinase(s) other than PKC.
SIMILARITY: Belongs to the GADD45 family.
WEB RESOURCE: Name=NIEHS-SNPs; URL=\'http://egp.gs.washington.edu/data/gadd45a/\';
WEB RESOURCE: Name=Wikipedia; Note=GADD45A entry; URL=\'http://en.wikipedia.org/wiki/GADD45A\';
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|
| Map of ordered and disordered regions |
Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.
|
| Region 1 | | Type: | Disordered - Extended | | Name: | N-terminal region | | Location: | 1 - 16 | | Length: | 16 | | Region sequence: |
MTLEEFSAGEQKTERM | | Modification type: | Monomeric
Native
| | PDB: | 2KG4:A | | Structural/functional type: | Relationship to function unknown
| | Functional classes: | Unknown
| | Functional subclasses: | Unknown
| Detection methods:
- Nuclear magnetic resonance (NMR) (298 K; pH: 7.4; gadd45a (concentration range: 0.16-0.45 mM); sodium phosphate 20 mM; potassium chloride 100 mM; DTT 2 mM; EDTA 2 mM; sodium azide 0.03 %; H2O/D2O (93%/7%))
- Analytical ultracentrifugation (298 K; pH: 7.4; DTT 2 mM; EDTA 2 mM; KCl 100 mM; sodium phosphate 20 mM)
| References:
- Sanchez R, Pantoja-Uceda D, Prieto J, Diercks T, Marcaida MJ, Montoya G, Campos-Olivas R, Blanco FJ. "Solution structure of human growth arrest and DNA damage 45alpha (Gadd45alpha) and its interactions with proliferating cell nuclear antigen (PCNA) and Aurora A kinase." J. Biol. Chem.. 2010; 285(29): 22196-201. PubMed: 20460379
| Comments:Sanchez et al (2010) report that the disordered N-terminal (aa 1-16) and the disordered loop between alpha-helix 4 and beta-sheet 4 (aa 105-118) both "show a propensity to form local helical structures in fast exchange with other conformations."
|
| Region 2 | | Type: | Disordered - Extended | | Name: | flexible loop | | Location: | 105 - 118 | | Length: | 14 | | Region sequence: |
ETDAGPAASEGAEQ | | Modification type: | Monomeric
Native
| | PDB: | 2KG4:A | | Structural/functional type: | Relationship to function unknown
| | Functional classes: | Molecular recognition effectors
| | Functional subclasses: | Apoptosis Regulation
Protein-protein binding
| Detection methods:
- Nuclear magnetic resonance (NMR) (298 K; pH: 7.4; DTT 2 mM; EDTA 2 mM; gadd45a (concentration range: 0.16-0.45 mM); H2O/D2O (93%/7%); potassium chloride 100 mM; sodium azide 0.03 %; sodium phosphate 20 mM)
- Analytical ultracentrifugation (298 K; pH: 7.4; DTT 2 mM; EDTA 2 mM; KCl 100 mM; sodium phosphate 20 mM)
| References:
- Sanchez R, Pantoja-Uceda D, Prieto J, Diercks T, Marcaida MJ, Montoya G, Campos-Olivas R, Blanco FJ. "Solution structure of human growth arrest and DNA damage 45alpha (Gadd45alpha) and its interactions with proliferating cell nuclear antigen (PCNA) and Aurora A kinase." J. Biol. Chem.. 2010; 285(29): 22196-201. PubMed: 20460379
| Comments:Sanchez et al (2010) report that the disordered N-terminal (aa 1-16) and the disordered loop between alpha-helix 4 and beta-sheet 4 (aa 105-118) both "show a propensity to form local helical structures in fast exchange with other conformations."
|
| Region 3 | | Type: | Disordered | | Name: | C-terminal region | | Location: | 154 - 165 | | Length: | 12 | | Region sequence: |
DQWVPVINLPER | | Modification type: | Monomeric
Native
| | PDB: | 2KG4:A | | Structural/functional type: | Relationship to function unknown
| | Functional classes: | Molecular recognition effectors
| | Functional subclasses: | Apoptosis Regulation
Protein-protein binding
| Detection methods:
- Nuclear magnetic resonance (NMR) (298 K; pH: 7.4; DTT 2 mM; EDTA 2 mM; gadd45a (concentration range: 0.16-0.45 mM); H2O/D2O (93%/7%); potassium chloride 100 mM; sodium azide 0.03 %; sodium phosphate 20 mM)
- Analytical ultracentrifugation (298 K; pH: 7.4; DTT 2 mM; EDTA 2 mM; KCl 100 mM; sodium phosphate 20 mM)
| References:
- Sanchez R, Pantoja-Uceda D, Prieto J, Diercks T, Marcaida MJ, Montoya G, Campos-Olivas R, Blanco FJ. "Solution structure of human growth arrest and DNA damage 45alpha (Gadd45alpha) and its interactions with proliferating cell nuclear antigen (PCNA) and Aurora A kinase." J. Biol. Chem.. 2010; 285(29): 22196-201. PubMed: 20460379
| Comments:
|
| References |
- Sanchez R, Pantoja-Uceda D, Prieto J, Diercks T, Marcaida MJ, Montoya G, Campos-Olivas R, Blanco FJ. "Solution structure of human growth arrest and DNA damage 45alpha (Gadd45alpha) and its interactions with proliferating cell nuclear antigen (PCNA) and Aurora A kinase." J. Biol. Chem.. 2010; 285(29): 22196-201. PubMed: 20460379
|
| Comments |
Sent for AV 1-27-2011 (PMID: 20460379)
|
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