| General information | | DisProt: | DP00723 | | Name: | Gibberellin receptor GID1A | | Synonym(s): | GID1A_ARATH
AtCXE10
Carboxylesterase 10
GID1-like protein 1
Protein GA INSENSITIVE DWARF 1A
AtGID1A
| | First appeared in release: | Release 6.00 (07/01/2012) | | UniProt: | Q9MAA7 | | UniGene: | | | SwissProt: | GID1A_ARATH | | TrEMBL: | | | NCBI (GI): | | | Source organism: | Arabidopsis thaliana (Mouse-ear cress) | | Sequence length: | 345 | | Percent disordered: | 100% | | Homologues: | |
| Native sequence |
10 20 30 40 50 60
| | | | | |
MAASDEVNLI ESRTVVPLNT WVLISNFKVA YNILRRPDGT FNRHLAEYLD RKVTANANPV - 60
DGVFSFDVLI DRRINLLSRV YRPAYADQEQ PPSILDLEKP VDGDIVPVIL FFHGGSFAHS - 120
SANSAIYDTL CRRLVGLCKC VVVSVNYRRA PENPYPCAYD DGWIALNWVN SRSWLKSKKD - 180
SKVHIFLAGD SSGGNIAHNV ALRAGESGID VLGNILLNPM FGGNERTESE KSLDGKYFVT - 240
VRDRDWYWKA FLPEGEDREH PACNPFSPRG KSLEGVSFPK SLVVVAGLDL IRDWQLAYAE - 300
GLKKAGQEVK LMHLEKATVG FYLLPNNNHF HNVMDEISAF VNAEC
|
| Functional narrative |
Function: Functions as soluble gibberellin (GA) receptor. GA is an essential hormone that regulates growth and development in plants. Binds with high affinity the biologically active gibberellin GA4, but has no affinity for the biologically inactive GAs. In response to GA, interacts with specific DELLA proteins, known as repressors of GA-induced growth, and targets them for degradation via proteasome. Seems to be required for GA signaling that controls root growth, seed germination, stem elongation and flower development. Partially redundant with GID1B and GID1C.
SUBUNIT: Interacts (via N-terminus) with the DELLA proteins GAI, RGA, RGL1, RGL2 and RGL3 (via N-terminus) in a GA-dependent manner. Q9LQT8:GAI; NbExp=6; IntAct=EBI-963597, EBI-963606; Q9STX3:GID2; NbExp=3; IntAct=EBI-963597, EBI-619033; Q9SLH3:RGA; NbExp=7; IntAct=EBI-963597, EBI-963624; Q8GXW1:RGL2; NbExp=3; IntAct=EBI-963597, EBI-963665;
Subcellular Location: Nucleus (By similarity).
TISSUE SPECIFICITY: Widely expressed.
DISRUPTION PHENOTYPE: No visible phenotype under normal growth condition.
SIMILARITY: Belongs to the 'GDXG' lipolytic enzyme family.
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|
| Map of ordered and disordered regions |
Note: 'Mouse' over a region to see the start and stop residues. Click on a region to see detailed information.
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| Region 1 | | Type: | Disordered | | Name: | | | Location: | 1 - 345 | | Length: | 345 | | Region sequence: |
MAASDEVNLIESRTVVPLNTWVLISNFKVAYNILRRPDGTFNRHLAEYLDRKVTANANPV
DGVFSFDVLIDRRINLLSRVYRPAYADQEQPPSILDLEKPVDGDIVPVILFFHGGSFAHS
SANSAIYDTLCRRLVGLCKCVVVSVNYRRAPENPYPCAYDDGWIALNWVNSRSWLKSKKD
SKVHIFLAGDSSGGNIAHNVALRAGESGIDVLGNILLNPMFGGNERTESEKSLDGKYFVT
VRDRDWYWKAFLPEGEDREHPACNPFSPRGKSLEGVSFPKSLVVVAGLDLIRDWQLAYAE
GLKKAGQEVKLMHLEKATVGFYLLPNNNHFHNVMDEISAFVNAEC | | Modification type: | Complex
| | PDB: | 2ZHI:A, 2ZSH:A | | Structural/functional type: | Function arises via a disorder to order transition
| | Functional classes: | Molecular recognition effectors
| | Functional subclasses: | Transactivation (transcriptional activation)
Substrate/ligand binding
Protein-protein binding
| Detection methods:
- X-ray crystallography (293 K; pH: 8; DTT 1 mM; GA3 or GA4 0.1 mM; GAI fragment (DELLA domain); GID1A; LiCl2 0.8 M; PEG 4000 26 %; Tris-HCl 0.1 M)
- Sensitivity to proteolysis (298 K; GA3 and GAI DELLA domain (in presence or absence) 100 uM; GST–GID1A 80 ug/mL; trypsin 2.5 ug/mL)
| References:
- Murase K, Hirano Y, Sun TP, Hakoshima T. "Gibberellin-induced DELLA recognition by the gibberellin receptor GID1." Nature. 2008; 456(7221): 459-63. PubMed: 19037309
| Comments:According to Murase et al (2008), "GID1A is highly sensitive to proteases in the unbound state but resistant in the GA-bound state and even more resistant in the DELLA-bound state."
PDB structure 2ZSH is a complex of Gibberellin receptor GID1A (DP00723), GA3 (Gibberellic Acid) and the DELLA domain of DELLA protein GAI (DP00724). PDB structure 2ZSI is a complex of Gibberellin receptor GID1A (DP00723), GA4 and the DELLA domain of DELLA protein GAI (DP00724).
Murase et al (2008) state the following, "Application of GA3 to Arabidopsis showed that it is an active GA, although it does not seem to be an endogenous hormone in this plant. GA4 is the major bioactive GA in Arabidopsis." However, "the bound GA3 superimposes onto GA4 with a small root mean squared deviation."
|
| References |
- Murase K, Hirano Y, Sun TP, Hakoshima T. "Gibberellin-induced DELLA recognition by the gibberellin receptor GID1." Nature. 2008; 456(7221): 459-63. PubMed: 19037309
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| Comments |
Av sent 6-21-2012 (PMID:19037309)
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